PRKG1 Antibody - #DF7018

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产品: PRKG1 抗体
货号: DF7018
描述: Rabbit polyclonal antibody to PRKG1
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: Q13976
RRID: AB_2838974

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
PRKG1 Antibody detects endogenous levels of total PRKG1.
RRID:
AB_2838974
引用格式: Affinity Biosciences Cat# DF7018, RRID:AB_2838974.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

cGK 1; cGK1; cGKI alpha; cGKI; cGKI beta; cGMP-dependent protein kinase 1; cGMP-dependent protein kinase I; DKFZp686K042; FLJ36117; KGP1_HUMAN; KGPB; MGC71944; pkg; PRKG1; PRKG1B; PRKGR1A; PRKGR1B;

抗原和靶标

免疫原:

A synthesized peptide derived from human PRKG1, corresponding to a region within C-terminal amino acids.

基因/基因ID:
PRKG1(5592)
描述:
Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Sensory system > Olfactory transduction.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Digestive system > Salivary secretion.

文献引用

1). PRICKLE1, a Wnt/PCP signaling component, is overexpressed and associated with inferior prognosis in acute myeloid leukemia. Journal of Translational Medicine, 2021 (PubMed: 34001134) [IF=7.4]

Application: WB    Species: human    Sample:

Fig. 7| High expression of PRICKEL1 accompanied with core PCP pathway components and the migration and invasion components up-regulation in AML patients. a The amounts of the indicated PCP proteins were analyzed by Western blotting in AML patients (n=5) and normal controls(n=3).
2). New sesquiterpenes and viridin derivatives from Penicillium sp. Ameliorates NAFLD by regulating the PINK1/Parkin mitophagy pathway. Bioorganic chemistry, 2024 (PubMed: 39047333) [IF=4.5]
3). Curcumin suppresses copper accumulation in non-small cell lung cancer by binding ATOX1. BMC pharmacology & toxicology, 2024 (PubMed: 39169392) [IF=2.9]

Application: WB    Species: human    Sample: A549 and H1299 cells

Fig. 1 Effects of curcumin on ATOX1-associated copper chaperones in A549 and H1299 cells. (A) The binding activity between curcumin and ATOX1 protein was analyzed using molecular docking. (B) Cell viability was measured by CCK-8. (C) Levels of ATOX1, ATP7A, and COX17 proteins were measured by western blotting. The levels of proteins were normalized by β-actin. The full-length blots were shown in Supplementary Fig. 1 and Fig. 2. (D) The semi-quantitative analysis of the blots was analyzed by ImageJ software. (E) Effects of DC-AC50 treatment on cell viability. Three parallel experiments were performed in cells. NS no significance. *p 
4). Network Pharmacology Prediction and Pharmacological Verification Mechanism of Yeju Jiangya Decoction on Hypertension. Evidence-based complementary and alternative medicine : eCAM, 2021 (PubMed: 34055010)

Application: WB    Species: Rat    Sample:

Figure 12. Effect of CIF on NOS3/GCα1/cGMP/PRKG1. (a) Representative results of NOS3/sGC/PRKG1 in Western blot; (b) cGMP in the aortic homogenate for ELISA; (c–f) protein level of NOS3, GC-α1, NOS3, GC-α2, and PRKG1. The data were expressed as mean ± SD. #P 

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