ADIPOQ Antibody - #DF7000

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产品: ADIPOQ 抗体
货号: DF7000
描述: Rabbit polyclonal antibody to ADIPOQ
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
蛋白号: Q15848
RRID: AB_2838956

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
ADIPOQ Antibody detects endogenous levels of total ADIPOQ.
RRID:
AB_2838956
引用格式: Affinity Biosciences Cat# DF7000, RRID:AB_2838956.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

30 kDa adipocyte complement related protein; 30 kDa adipocyte complement-related protein; ACDC; ACRP30; ADIPO_HUMAN; Adipocyte; Adipocyte C1q and collagen domain containing protein; Adipocyte complement related 30 kDa protein; Adipocyte complement related protein of 30 kDa; Adipocyte complement-related 30 kDa protein; adipocyte-specific secretory protein; Adiponectin; Adiponectin precursor; adiponectin, C1Q and collagen domain containing; Adipoq; Adipose most abundant gene transcript 1; Adipose most abundant gene transcript 1 protein; Adipose specific collagen like factor; ADIPQTL1; ADPN; APM 1; apM-1; APM1; C1q and collagen domain-containing protein; GBP28; Gelatin binding protein; Gelatin binding protein 28; Gelatin-binding protein;

抗原和靶标

免疫原:

A synthesized peptide derived from human ADIPOQ, corresponding to a region within C-terminal amino acids.

基因/基因ID:
ADIPOQ(9370)
描述:
Adiponectin, also termed AdipoQ, Acrp30, apM1 and GBP28, is an adipokine expressed exclusively in brown and white adipocytes (1). It is secreted into the blood and exists in three major forms: a low molecular weight trimer, a medium molecular weight hexamer and a high molecular weight multimer (1). Adiponectin levels are decreased in obese and insulin-resistant mice and humans (2), suggesting that this adipokine is critical to maintain insulin sensitivity. Adiponectin stimulates the phosphorylation of AMPKα at Thr172 and activates AMPK in skeletal muscle (3). It also stimulates glucose uptake in myocytes (3). The block of AMPK activation by a dominant-negative AMPKα2 isoform inhibits the effect of adiponectin on glucose uptake, indicating that adiponectin stimulates glucose uptake and increases insulin sensitivity through its action on AMPK (3). Adiponectin mutants that are not able to form oligomers larger than trimers have no effect on the AMPK pathway (4). Mutations that render adiponectin unable to form high molecular weight multimers are associated with human diabetes (4), indicating the importance of multimerization for adiponectin activity.

研究领域

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

文献引用

1). Single xenotransplant of rat brown adipose tissue prolonged the ovarian lifespan of aging mice by improving follicle survival. Aging Cell, 2019 (PubMed: 31389140) [IF=8.0]

Application: WB    Species: mouse    Sample: lung

FIGURE 1|Rat‐to‐mouse (RTM) xenotransplanted brown adipose tissue (BAT) was functional well and did not cause injurious histocompatibility in aging mice.(h) HLA‐A blot showed that HLA‐A level in BAT of RTM and MTM groups was significantly higher than aging or young group; HLA‐A level in the lung of young group is significantly low; HLA‐A levels in other main tissues (liver,spleen, brain, kidney, heart) are similar among all four groups.
2). Chondrocyte Adipogenic Differentiation in Softening Osteoarthritic Cartilage. Journal of dental research, 2022 (PubMed: 34903082) [IF=5.7]
3). Aerobic Exercise Prevents Arterial Stiffness and Attenuates Hyperexcitation of Sympathetic Nerves in Perivascular Adipose Tissue of Mice after Transverse Aortic Constriction. International Journal of Molecular Sciences, 2022 (PubMed: 36232489) [IF=5.6]

Application: WB    Species: Mouse    Sample:

Figure 5 Effects of exercise on the expressions of β3-AR and adiponectin in PVAT of mice after TAC. Representative Western blot assessment of β3-AR (A) and adiponectin (B) expressions in PVAT normalized to the expressions of GAPDH. Data were analyzed using one-way ANOVA; values are mean ± SD. * indicates p < 0.05. Abbreviations: β3-AR, β3-adrenergic receptor; SHAM, sham surgery; TAC-SE, transverse aortic constriction-sedentary; and TAC-EX, transverse aortic constriction-exercise.
4). Zonal interdependence in the temporomandibular joint cartilage. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2023 (PubMed: 36961420) [IF=4.4]
5). Autologous decellularized extracellular matrix promotes adipogenic differentiation of adipose derived stem cells in low serum culture system by regulating the ERK1/2-PPARγ pathway. Adipocyte, 2021 (PubMed: 33825675) [IF=3.5]

Application: WB    Species: Human    Sample: ADSCs

Figure 7. Effects of d-ECM on the ERK1/2-PPARγ pathway and the expression of adipocyte secreting factors ADIPOQ and aP2 in the fully differentiated ADSCs. After 3-days treatments and 14-days adipogenic induction, ADSCs at the 5th passage were collected and used for Western blotting analysis (a). Protein levels of ERK1/2 (b), p-ERK1/2 (c), PPARγ (d), p-PPARγ (e), ADIPOQ (f) and aP2 (g) were examined. GAPDH demonstrated the equal loading of protein samples. N = 3. *, p < 0.05, vs. 10% FBS group; **, p < 0.01, vs. 10% FBS group; #, p < 0.05, vs. 2% FBS group; ##, p < 0.01, vs. 2% FBS group; $, p < 0.05, vs. 2% FBS + d-ECM group; $$, p < 0.01, vs. 2% FBS + d-ECM group. ADIPOQ, adiponectin; aP2, adipocyte fatty-acid binding protein

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