产品: Claudin 10 抗体
货号: AF0133
描述: Rabbit polyclonal antibody to Claudin 10
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 25kDa; 24kD(Calculated).
蛋白号: P78369
RRID: AB_2833317

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(86%)
克隆:
Polyclonal
特异性:
Claudin 10 Antibody detects endogenous levels of total Claudin 10.
RRID:
AB_2833317
引用格式: Affinity Biosciences Cat# AF0133, RRID:AB_2833317.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Claudin-10; Claudin10; CLD10_HUMAN; CLDN 10; CLDN10; CLDN10 protein; CPETRL 3; CPETRL3; HGNC:2033; OSP L; OSP like protein; OSP-like protein; OSPL;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P78369 CLD10_HUMAN:

Expressed in the kidney, eccrine sweat glands and in all layers of the epidermis. In the kidney, it is detected in the thick ascending limb of Henle's loop (TAL) (PubMed:28771254, PubMed:28686597). In the sweat glands, it is expressed in cells from secretory portions, corresponding to the clear cells (PubMed:28686597).

描述:
claudin 10 Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Belongs to the claudin family.
序列:
MASTASEIIAFMVSISGWVLVSSTLPTDYWKVSTIDGTVITTATYWANLWKACVTDSTGVSNCKDFPSMLALDGYIQACRGLMIAAVSLGFFGSIFALFGMKCTKVGGSDKAKAKIACLAGIVFILSGLCSMTGCSLYANKITTEFFDPLFVEQKYELGAALFIGWAGASLCIIGGVIFCFSISDNNKTPRYTYNGATSVMSSRTKYHGGEDFKTTNPSKQFDKNAYV

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
86
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P78369 作为底物

Site PTM Type Enzyme
S94 Phosphorylation
Y192 Phosphorylation
T193 Phosphorylation
Y194 Phosphorylation
S199 Phosphorylation
S202 Phosphorylation
S203 Phosphorylation
T205 Phosphorylation
Y207 Phosphorylation
Y227 Phosphorylation

研究背景

功能:

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. Involved in the regulation of paracellular epithelia permeability to ions in multiple organs. It acts as a paracellular ion channel probably forming permselective pores; isoform 1 appears to create pores preferentially permeable to cations and isoform 2 for anions. In sweat glands and in the thick ascending limb (TAL) of Henle's loop in kidney, it controls paracellular sodium permeability which is essential for proper sweat production and renal function.

细胞定位:

Cell junction>Tight junction. Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in the kidney, eccrine sweat glands and in all layers of the epidermis. In the kidney, it is detected in the thick ascending limb of Henle's loop (TAL). In the sweat glands, it is expressed in cells from secretory portions, corresponding to the clear cells.

亚基结构:

Can form homodimers both in trans (interaction between CLDN10 molecules in opposing membranes) and in cis (interaction between CLDN10 molecules within one membrane).

蛋白家族:

The fourth transmembrane region (161-181) is necessary for integration into tight junctions.

Belongs to the claudin family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

文献引用

1). Claudin-10 overexpression suppresses human clear cell renal cell carcinoma growth and metastasis by regulating ATP5O and causing mitochondrial dysfunction. International Journal of Biological Sciences, 2022 (PubMed: 35414767) [IF=9.2]

Application: IHC    Species: mouse    Sample: renal

Figure 1. |CLDN10 is low expressed in ccRCC and decreased CLDN10 expression is associated with ccRCC metastasis and poor prognosis. A, CLDN10 expression was analyzed by immunohistochemistry in 54 pairs of paraffin sections of ccRCC and adjacent normal renal tissues (AN).

2). CLDN10 associated with immune infiltration is a novel prognostic biomarker for clear cell renal cell carcinoma. Epigenomics, 2021 (PubMed: 33203244) [IF=3.8]

Application: WB    Species: Human    Sample: renal tubular epithelial cell

Figure 2. Expression profile of CLDN10 in mccRCC tissues and cell line, and its relationship with overall survival (OS) and disease-free survival (DFS). (A–C) Plots showing the expression profile of CLDN10 in mccRCC tissues and AN based on the data from TCGA-KIRC, GSE66271 and GSE105288, respectively. (D) Plot showing the expression profile of CLDN10 in mccRCC cell line Caki-1 and healthy kidney epithelial cell line (ASE-5063) based on the data from GSE78179. (E) Kaplan-Meier curves of OS and DFS in 80 and 53 mccRCC patients, respectively. Patients were separated into two groups according to the median cutoff of CLDN10 expression. (F) Heatmap of CLDN10 mRNA expression in 54 paired ccRCC tissues. (G) Statistical comparison of CLDN10 mRNA expression between ccRCC tissues and AN. (H) Statistical comparison of CLDN10 mRNA expression between mccRCC tissues (M1) and ccRCC tissues (M0). (I) The expression of CLDN10 protein in HK2, 786-O, A498 and Caki-1 cell lines. AN: Adjacent normal tissues; DFS: Disease-free survival; mccRCC: Metastatic clear cell renal cell carcinoma; OS: Overall survival.

限制条款

产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)

产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.