ACK1 Antibody - #DF6987

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产品: ACK1 抗体
货号: DF6987
描述: Rabbit polyclonal antibody to ACK1
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Rabbit, Dog, Chicken, Xenopus
蛋白号: Q07912
RRID: AB_2838943

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
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说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
ACK1 Antibody detects endogenous levels of total ACK1.
RRID:
AB_2838943
引用格式: Affinity Biosciences Cat# DF6987, RRID:AB_2838943.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Acetate kinase 1; Acetokinase 1; ACK 1; ACK; ACK-1; ACK1; ACK1_HUMAN; Activated Cdc42 associated kinase 1; Activated CDC42 kinase 1; Activated p21cdc42Hs kinase; FLJ44758; FLJ45547; p21cdc42Hs; TNK 2; TNK2; Tyrosine kinase non receptor 2; Tyrosine kinase non receptor protein 2; Tyrosine kinase non-receptor protein 2;

抗原和靶标

免疫原:

A synthesized peptide derived from human ACK1, corresponding to a region within C-terminal amino acids.

基因/基因ID:
TNK2(10188)
描述:
This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.

文献引用

1). Significant gene biomarker Tyrosine Kinase Non-receptor 2 mediated cell proliferation and invasion in Colon Cancer. Frontiers in Genetics, 2021 (PubMed: 34421982) [IF=3.7]

Application: WB    Species: Human    Sample: colon cancer cells

Figure 2 TNK2 was overexpressed and directly binds to miR-125a-3p in colon cancer cells. (A,B) Relative expression of TNK2 in COAD and normal colon cells, measured by RT-qPCR and western blot. (C,D) Relative expression of TNK2 after transfection of SW620 cells was measured by RT-qPCR. (E) Putative binding sites involving the TNK2 3′UTR and miR-125a-3p were predicted by miRBase. (F) Dual-luciferase reporter assays were performed to test putative binding sites in TNK2 3′UTR and miR-125a-3p. (G) Expression levels of miR-125a-3p in COAD and normal colon tissue, analyzed using UALCAN. (H) Relative expression of miR-125a-3p in COAD and normal colon cells, as measured by RT-qPCR. (I,J) Relative miR-125a-3p expression after transfection of SW620 cells was measured by RT-qPCR. (K,L) Relative TNK2 mRNA and protein expression were detected by RT-qPCR and western blotting after transfection. *p < 0.05, **p < 0.01.

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