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| 产品: | ADAMTS4 抗体 |
| 货号: | DF6986 |
| 描述: | Rabbit polyclonal antibody to ADAMTS4 |
| 应用: | WB IHC |
| 文献验证: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 蛋白号: | O75173 |
| RRID: | AB_2838942 |
产品描述
来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
ADAMTS4 Antibody detects endogenous levels of total ADAMTS4.
RRID:
AB_2838942
引用格式: Affinity Biosciences Cat# DF6986, RRID:AB_2838942.
引用格式: Affinity Biosciences Cat# DF6986, RRID:AB_2838942.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
A disintegrin and metalloproteinase with thrombospondin motifs 4; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4; A disintegrin like and metalloprotease with thrombospondin type 1 motif 4; ADAM metallopeptidase with thrombospondin type 1 motif 4; ADAM TS 4; ADAM TS4; ADAM-TS 4; ADAM-TS4; ADAMTS 2; ADAMTS 4; ADAMTS-4; ADAMTS2; ADAMTS4; ADAMTS4 protein; ADMP 1; ADMP-1; ADMP1; Aggrecanase 1; Aggrecanase-1; Aggrecanase1; ATS4_HUMAN; KIAA0688;
抗原和靶标
免疫原:
A synthesized peptide derived from human ADAMTS4, corresponding to a region within the internal amino acids.
基因/基因ID:
描述:
This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.
文献引用
1). Pharmacological network analysis of the functions and mechanism of kaempferol from Du Zhong in intervertebral disc degeneration (IDD). Journal of orthopaedic translation, 2023
(PubMed: 36909862)
[IF=5.9]
2). PD184352 exerts anti-inflammatory and antioxidant effects by promoting activation of the Nrf2/HO-1 axis. Biochemical Pharmacology, 2023
(PubMed: 37028460)
[IF=5.3]
3). Casticin ameliorates osteoarthritic cartilage damage in rats through PI3K/AKT/HIF-1α signaling. Chemico-biological interactions, 2024
(PubMed: 38309612)
[IF=4.7]
4). Medical Prospect of Melatonin in the Intervertebral Disc Degeneration through Inhibiting M1-Type Macrophage Polarization via SIRT1/Notch Signaling Pathway. Biomedicines, 2023
(PubMed: 37371708)
[IF=4.7]
Application: WB Species: Human Sample: NP cells
Figure 4 Protection on NP cells against the CM-induced inflammation and imbalanced ECM homeostasis by the treatment of MLT. NP cells were treated with different CMs collected from the LPS-stimulated RAW 264.7 Mφs in the presence or absence of MLT for 24 h, then followed by different analysis. (A) The relative mRNA levels of pro-inflammation cytokines in NP cells were measured by RT-qPCR analyses (n = 3). The levels of IL-6, TNF-α, iNOS and COX-2 mRNA declined in the presence of MLT treatment to Mφs at different dosages. (B) The relative mRNA levels of matrix anabolic and degrading enzymes in NP cells were measured by RT-qPCR analyses (n = 3). The levels of COL2A1 and TIMP-1 mRNA were upregulated, while the levels of MMP-13, ADAMTS4 and ADAMTS5 mRNA were downregulated in the presence of MLT treatment to Mφs at different dosages. (C,D) The relative protein levels of pro-inflammation cytokines in NP cells were measured by Western blot analyses (n = 3). (C) Western blotting showed the decline of inflammation-associated proteins. (D) Quantitative analysis showed the reduced levels of IL-6, TNF-α, iNOS and COX-2 protein after treatment with MLT to Mφs at different dosages. (E,F) The relative protein levels of matrix anabolic and degrading enzymes in NP cells were measured by Western blot analyses (n = 3). (E) Western blotting showed the different changes of ECM protein productions in NP cells. (F) Quantitative analysis showed the increased levels of COL2A1 and TIMP-1 protein, and the reduced levels of MMP-13, ADAMTS4 and ADAMTS5 protein after the treatment with MLT to Mφs at different dosages. Data are expressed as mean ± standard deviation. The two groups among the five groups are compared by using an independent t-test.
5). AMPK deficiency in chondrocytes accelerated the progression of instability-induced and ageing-associated osteoarthritis in adult mice. Scientific Reports, 2017
(PubMed: 28225087)
[IF=3.8]
6). Omentin-1 promoted proliferation and ameliorated inflammation, apoptosis, and degeneration in human nucleus pulposus cells. ARCHIVES OF GERONTOLOGY AND GERIATRICS, 2022
(PubMed: 35704952)
[IF=3.5]
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