AXIN2 Antibody - #DF6978

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产品: AXIN2 抗体
货号: DF6978
描述: Rabbit polyclonal antibody to AXIN2
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken
分子量: 93kDa; 94kD(Calculated).
蛋白号: Q9Y2T1
RRID: AB_2838934

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Zebrafish(%), Bovine(%), Horse(%), Rabbit(%), Dog(%), Chicken(%)
克隆:
Polyclonal
特异性:
AXIN2 Antibody detects endogenous levels of total AXIN2.
RRID:
AB_2838934
引用格式: Affinity Biosciences Cat# DF6978, RRID:AB_2838934.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Axil; Axin like protein; Axin-2; Axin-like protein; Axin2; AXIN2_HUMAN; Axis inhibition protein 2; Conductin; DKFZp781B0869; MGC10366; MGC126582;

抗原和靶标

免疫原:
Uniprot:

Q9Y2T1(AXIN2_HUMAN) >>Visit HPA database.

基因/基因ID:
AXIN2(8313)
表达:
Q9Y2T1 AXIN2_HUMAN:

Expressed in brain and lymphoblast.

描述:
The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.
序列:
MSSAMLVTCLPDPSSSFREDAPRPPVPGEEGETPPCQPGVGKGQVTKPMSVSSNTRRNEDGLGEPEGRASPDSPLTRWTKSLHSLLGDQDGAYLFRTFLEREKCVDTLDFWFACNGFRQMNLKDTKTLRVAKAIYKRYIENNSIVSKQLKPATKTYIRDGIKKQQIDSIMFDQAQTEIQSVMEENAYQMFLTSDIYLEYVRSGGENTAYMSNGGLGSLKVVCGYLPTLNEEEEWTCADFKCKLSPTVVGLSSKTLRATASVRSTETVDSGYRSFKRSDPVNPYHIGSGYVFAPATSANDSEISSDALTDDSMSMTDSSVDGIPPYRVGSKKQLQREMHRSVKANGQVSLPHFPRTHRLPKEMTPVEPATFAAELISRLEKLKLELESRHSLEERLQQIREDEEREGSELTLNSREGAPTQHPLSLLPSGSYEEDPQTILDDHLSRVLKTPGCQSPGVGRYSPRSRSPDHHHHHHSQYHSLLPPGGKLPPAAASPGACPLLGGKGFVTKQTTKHVHHHYIHHHAVPKTKEEIEAEATQRVHCFCPGGSEYYCYSKCKSHSKAPETMPSEQFGGSRGSTLPKRNGKGTEPGLALPAREGGAPGGAGALQLPREEGDRSQDVWQWMLESERQSKPKPHSAQSTKKAYPLESARSSPGERASRHHLWGGNSGHPRTTPRAHLFTQDPAMPPLTPPNTLAQLEEACRRLAEVSKPPKQRCCVASQQRDRNHSATVQTGATPFSNPSLAPEDHKEPKKLAGVHALQASELVVTYFFCGEEIPYRRMLKAQSLTLGHFKEQLSKKGNYRYYFKKASDEFACGAVFEEIWEDETVLPMYEGRILGKVERID

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Zebrafish
92
Chicken
92
Sheep
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B.

翻译修饰:

Probably phosphorylated by GSK3B and dephosphorylated by PP2A.

ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway.

Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in brain and lymphoblast.

亚基结构:

Interacts with glycogen synthase kinase-3 beta (GSK3B) and beta-catenin. The interaction between axin and beta-catenin occurs via the armadillo repeats contained in beta-catenin (By similarity). Interacts with SMAD7 and RNF111. Interacts with ANKRD6.

蛋白家族:

The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

文献引用

1). Target deubiquitinase OTUB1 as a therapeatic strategy for BLCA via β-catenin/necroptosis signal pathway. International journal of biological sciences, 2024 (PubMed: 39113709) [IF=8.2]
2). Hederagenin ameliorates cisplatin-induced acute kidney injury via inhibiting long non-coding RNA A330074k22Rik/Axin2/β-catenin signalling pathway. International Immunopharmacology, 2022 (PubMed: 36155281) [IF=4.8]

Application: WB    Species: Mice    Sample: pTEC cells

Fig. 5. Transcriptome sequencing analysis of differential mRNAs after downregulation of A33 in LPS-stimulated pTEC cells (A) Violin plot of gene expression; (B) Based on the results of differential analysis, we screened genes with FDR < 0.05 and |log2FC|>1 as significantly differential genes, and the statistical histogram of differential genes. (C) The different genes volcano plot of LPS + siR-A33 vs LPS; (D) KEGG enrichment: the top 20 pathways with the smallest Q value. Inflammation-related pathways are in the red box, and pathways in which Wnt signaling first appears are in the blue box; (E) Heatmap of gene expression associated with wnt/β-catenin signaling; (F) Western blot analysis of Axin2 and β-catenin protein levels after down-regulation of A33. ***P < 0.001 vs LPS group.

Application: IHC    Species: Mice    Sample:

Fig. 6. HDG strongly inhibited the expression of Axin2 and β-catenin in vivo and in vitro, and A33 was the upstream regulatory LncRNA of Axin2 and β-catenin. (A) Immunohistochemistry results for Axin2 and β-catenin in each group; (B,C) Real-time PCR and WB results confirmed that mRNA and protein levels of Axin and β-catenin decreased significantly in HDG treatment group; (D) Immunohistochemistry results of Axin2 and β-catenin after knockdown of A33 in vivo; (E,F) Real-time PCR and WB results confirmed that HDG had a significantly inhibitory effect on Axin2 and β-catenin in LPS-induced pTEC; (G,H) Real-time PCR and WB confirmed that the expression of Axin2/β-catenin was synergistic with the expression of A33 after knockdown and over-expression in vitro. *P < 0.05, **P < 0.01, ***P < 0.001 vs Cis/LPS group; #P < 0.05, ###P < 0.001 vs Ctrl group.
3). Ubiquitin-associated protein 2 like (UBAP2L) enhances growth and metastasis of gastric cancer cells. Bioengineered, 2021 (PubMed: 34823423) [IF=4.2]
4). miR-140-y targets TCF4 to regulate the Wnt signaling pathway and promote embryonic feather follicle development in Hungarian white goose. Poultry science, 2024 (PubMed: 38350393) [IF=3.8]
5). The role and mechanism of β-catenin-mediated skeletal muscle satellite cells in osteoporotic fractures by Jian-Pi-Bu-Shen formula. Journal of molecular histology, 2024 (PubMed: 39105942) [IF=2.9]
6). HDAC1 regulates inflammation and osteogenic differentiation of ankylosing spondylitis fibroblasts through the Wnt-Smad signaling pathway. Journal of Orthopaedic Surgery and Research, 2022 (PubMed: 35794630) [IF=2.8]

Application: WB    Species: Human    Sample: AS fibroblasts

Fig. 3 Proteins expression of Wnt and Smad signaling pathways in AS fibroblasts after different treatment. A Protein expressions of Wnt, β-catenin, GSK-3β, Axin, Smad3 and p-Smad3 in AS fibroblasts were detected by western blot analysis. B Densitometry analysis of Wnt protein expression. C Densitometry analysis of β-catenin protein expression. D Densitometry analysis of GSK-3β protein expression. E Densitometry analysis of Axin protein expression. F Densitometry analysis of p-Smad/Smad protein expression. Data were shown as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001, compared with the AAV-NC group. #P < 0.05, ##P < 0.01 and ###P < 0.001, compared with the AAV-HDAC1 group. &P < 0.05, &&P < 0.01 and &&&P < 0.001, compared with the AAV-HDAC1 + DKK-1 group
7). Gigantol Attenuates the Metastasis of Human Bladder Cancer Cells, Possibly Through Wnt/EMT Signaling. OncoTargets and Therapy, 2020 (PubMed: 33177841) [IF=2.7]

Application: WB    Species: Human    Sample: Bladder cancer cell

Figure 3 Cell invasion assays and expression analysis of Wnt/EMT related genes in cells treated with gigantol. (A) Cell invasion was measured by transwell assay in bladder cancer cells treated with increasing concentrations of gigantol (magnification ×100). (B) qRT-PCR analysis of the Wnt target genes and EMT markers in bladder cancer cells treated with gigantol. (C) Western blot analysis of Wnt/EMT markers in bladder cancer cells treated with indicated concentrations of gigantol. Data in (B) were shown as means ± SD (n = 3), the statistically significant differences were considered at *p<0.05, **p<0.01, ***p<0.001.
8). Wnt and Smad signaling pathways synergistically regulated the osteogenic differentiation of fibroblasts in ankylosing spondylitis. TISSUE & CELL, 2022 (PubMed: 35753224) [IF=2.7]
9). Golden pompano genome resource enables discovery of valuable gene determining growth traits. bioRxiv, 2021

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