EEF1D Antibody - #DF6974

(1)  
产品: EEF1D 抗体
货号: DF6974
描述: Rabbit polyclonal antibody to EEF1D
应用: WB IHC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 31kDa,37kDa; 31kD(Calculated).
蛋白号: P29692
RRID: AB_2838930

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
EEF1D Antibody detects endogenous levels of total EEF1D.
RRID:
AB_2838930
引用格式: Affinity Biosciences Cat# DF6974, RRID:AB_2838930.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Antigen NY CO 4; Antigen NY-CO-4; EEF 1D; EEF1D; EF 1 delta; EF 1D; EF-1-delta; EF1D; EF1D_HUMAN; Elongation factor 1 delta; Elongation factor 1-delta; eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein); Eukaryotic translation elongation factor 1 delta; FP1047; Guanine nucleotide exchange protein;

抗原和靶标

免疫原:
Uniprot:

P29692(EF1D_HUMAN) >>Visit HPA database.

基因/基因ID:
EEF1D(1936)
表达:
P29692 EF1D_HUMAN:

Isoform 2 is specifically expressed in brain, cerebellum and testis.

描述:
This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.
序列:
MATNFLAHEKIWFDKFKYDDAERRFYEQMNGPVAGASRQENGASVILRDIARARENIQKSLAGSSGPGASSGTSGDHGELVVRIASLEVENQSLRGVVQELQQAISKLEARLNVLEKSSPGHRATAPQTQHVSPMRQVEPPAKKPATPAEDDEDDDIDLFGSDNEEEDKEAAQLREERLRQYAEKKAKKPALVAKSSILLDVKPWDDETDMAQLEACVRSIQLDGLVWGASKLVPVGYGIRKLQIQCVVEDDKVGTDLLEEEITKFEEHVQSVDIAAFNKI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P29692 作为底物

Site PTM Type Enzyme
A2 Acetylation
K10 Acetylation
K10 Ubiquitination
K15 Acetylation
K15 Ubiquitination
K17 Acetylation
K17 Ubiquitination
Y18 Phosphorylation
R24 Methylation
Y26 Phosphorylation
S37 Phosphorylation
S44 Phosphorylation
K59 Ubiquitination
S60 Phosphorylation
S64 Phosphorylation
S65 Phosphorylation
S70 Phosphorylation
T73 Phosphorylation
S74 Phosphorylation
S86 Phosphorylation
S93 Phosphorylation
R95 Methylation
S106 Phosphorylation
K107 Acetylation
K107 Ubiquitination
K117 Acetylation
K117 Ubiquitination
S118 Phosphorylation
S119 Phosphorylation
T125 Phosphorylation
T129 Phosphorylation
S133 Phosphorylation P06493 (CDK1)
T147 Phosphorylation
S162 Phosphorylation P68400 (CSNK2A1)
K169 Ubiquitination
R178 Methylation
Y182 Phosphorylation
K185 Ubiquitination
K186 Ubiquitination
K188 Ubiquitination
K189 Ubiquitination
S197 Phosphorylation
K203 Acetylation
K203 Sumoylation
S220 Phosphorylation
S231 Phosphorylation
K232 Ubiquitination
Y238 Phosphorylation
K242 Ubiquitination
K265 Ubiquitination
K280 Ubiquitination

研究背景

功能:

EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.

Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE).

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Isoform 2 is specifically expressed in brain, cerebellum and testis.

亚基结构:

EF-1 is composed of 4 subunits: alpha, beta, delta isoform 1, and gamma. Isoform 2 interacts with HSF1 and NFE2L2.

蛋白家族:

Belongs to the EF-1-beta/EF-1-delta family.

研究领域

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.