PSMC4 Antibody - #DF6973

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产品: PSMC4 抗体
货号: DF6973
描述: Rabbit polyclonal antibody to PSMC4
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 47kDa; 47kD(Calculated).
蛋白号: P43686
RRID: AB_2838929

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
PSMC4 Antibody detects endogenous levels of total PSMC4.
RRID:
AB_2838929
引用格式: Affinity Biosciences Cat# DF6973, RRID:AB_2838929.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

26S protease regulatory subunit 6B; 26S proteasome AAA ATPase subunit RPT3; 26S proteasome AAA-ATPase subunit RPT3; MB67 interacting protein; MB67-interacting protein; MIP224; Protease 26S subunit 6; Proteasome (prosome macropain) 26S subunit ATPase 4; Proteasome 19S S6; Proteasome 26S subunit ATPase 4; Proteasome 26S subunit, ATPase, 4; PRS6B_HUMAN; PSMC4; RPT3; S6; Tat binding protein 7; TAT-binding protein 7; TBP 7; TBP-7;

抗原和靶标

免疫原:
Uniprot:

P43686(PRS6B_HUMAN) >>Visit HPA database.

基因/基因ID:
PSMC4(5704)
描述:
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the triple-A family of ATPases that is a component of the 19S regulatory subunit and plays a role in 26S proteasome assembly. The encoded protein interacts with gankyrin, a liver oncoprotein, and may also play a role in Parkinson's disease through interactions with synphilin-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
序列:
MEEIGILVEKAQDEIPALSVSRPQTGLSFLGPEPEDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRIQSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDVMYADIGGMDIQKQEVREAVELPLTHFELYKQIGIDPPRGVLMYGPPGCGKTMLAKAVAHHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKENAPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQNVNVKVIMATNRADTLDPALLRPGRLDRKIEFPLPDRRQKRLIFSTITSKMNLSEEVDLEDYVARPDKISGADINSICQESGMLAVRENRYIVLAKDFEKAYKTVIKKDEQEHEFYK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P43686 作为底物

Site PTM Type Enzyme
M1 Acetylation
S21 Phosphorylation
T25 Phosphorylation Q92630 (DYRK2)
S28 Phosphorylation
Y41 Phosphorylation
K45 Ubiquitination
K46 Ubiquitination
K62 Ubiquitination
K66 Ubiquitination
K70 Ubiquitination
K80 Ubiquitination
S84 Phosphorylation
Y111 Phosphorylation
Y112 Phosphorylation
S117 Phosphorylation
T118 Phosphorylation
K125 Ubiquitination
K134 Methylation
Y164 Phosphorylation
K174 Ubiquitination
K192 Ubiquitination
Y205 Phosphorylation
K212 Ubiquitination
K217 Ubiquitination
K238 Acetylation
K238 Ubiquitination
K255 Ubiquitination
K273 Ubiquitination
R283 Methylation
T316 Phosphorylation
K330 Ubiquitination
R338 Methylation
S346 Phosphorylation
T347 Phosphorylation
S350 Phosphorylation
K397 Acetylation
K397 Ubiquitination
K401 Acetylation
K401 Ubiquitination
K404 Ubiquitination
K408 Ubiquitination
K409 Ubiquitination
Y417 Phosphorylation
K418 Acetylation
K418 Ubiquitination

研究背景

功能:

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC4 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.

细胞定位:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases including PSMC4 and few additional components. Interacts with NR1I3. Interacts with PAAF1. Interacts with TRIM5. Interacts with ZFAND1.

蛋白家族:

Belongs to the AAA ATPase family.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > Proteasome.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

文献引用

1). L-leucine dietary supplementation modulates muscle protein degradation and increases pro-inflammatory cytokines in tumour-bearing rats. CYTOKINE (PubMed: 28494385) [IF=3.8]

Application: WB    Species: rat    Sample: gastrocnemius muscle tissue

Fig. 3.| Muscle mass-to-body weight ratio (%; A), total muscle protein concentrations (µg/µL; B) and proteasome subunits: 11S (% of arbitrary unity; C), 19S (% of arbitrary unity; D), and 20Sα (subunits 29 kDa and 32 kDa; (% of arbitrary unity; E)) in the gastrocnemius muscle tissue of Wistar rats with or without tumour and leucine nutritional supplementation. Data are plotted as the mean ± SD. Legend: C: Rats fed a normal protein diet; L: Rats fed a leucine-rich diet; W:Rats fed a normal protein diet and inoculated with Walker 256 tumour cells; WL:Rats fed a leucine-rich diet and inoculated with Walker 256 tumour cells. Samples were collected on the 7th, 14th and 21st days after the beginning of the experiment. **Significant differences as a result of tumour effects, two-way ANOVA with Bonferroni testing, n = 6 per group; *Significant difference as a result of leucine modulation of tumour effects, two-way ANOVA with Bonferroni testing, n = 6 per group. P ≤ 0.05.

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