Tri-Methyl-Histone H3 (Lys9)/H3K9me3 Antibody - #DF6938

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产品: Tri-Methyl-Histone H3 (Lys9)/H3K9me3 抗体
货号: DF6938
描述: Rabbit polyclonal antibody to Tri-Methyl-Histone H3 (Lys9)/H3K9me3
应用: WB IHC IF/ICC IP CHIP
文献验证: IF/ICC
反应: Human, Mouse, Rat, Monkey
预测: Horse, Sheep, Rabbit
蛋白号: Q16695
RRID: AB_2838897

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 50ul RMB¥ 1250 现货
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:50-1:200, IP 1:50-1:200, CHIP 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
克隆:
Polyclonal
特异性:
Tri-Methyl-Histone H3 (Lys9)/H3K9me3 Antibody detects endogenous levels of Tri-Methyl-Histone H3 only when methylated at Lys9.
RRID:
AB_2838897
引用格式: Affinity Biosciences Cat# DF6938, RRID:AB_2838897.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

;H3.3A; HIST1 cluster, H3E; H3 histone family, member A; H3.1; H3/l; H3F3; H3FF; H3FJ; H3FL; Histone gene cluster 1, H3 histone family, member E; histone H3.1t; Histone H3/o; FLJ92264; H 3; H3; H3 histone family, member B; H3 histone family, member C; H3 histone family, member D; H3 histone family, member F; H3 histone family, member H; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member T; H3 histone, family 3A; H3/A; H3/b; H3/c; H3/d; h3/f; H3/h; H3/i; H3/j; H3/k; H3/t; H31_HUMAN; H3F1K; H3F3A; H3FA; H3FB; H3FC; H3FD; H3FH; H3FI; H3FK; HIST1 cluster, H3A; HIST1 cluster, H3B; HIST1 cluster, H3C; HIST1 cluster, H3D; HIST1 cluster, H3F; HIST1 cluster, H3G; HIST1 cluster, H3H; HIST1 cluster, H3I; HIST1 cluster, H3J; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; Histone 1, H3a; Histone 1, H3b; Histone 1, H3c; Histone 1, H3d; Histone 1, H3e; Histone 1, H3f; Histone 1, H3g; Histone 1, H3h; Histone 1, H3i; Histone 3, H3; histone cluster 1 H3 family member a; histone cluster 1 H3 family member b; histone cluster 1 H3 family member c; histone cluster 1 H3 family member d; histone cluster 1 H3 family member e; histone cluster 1 H3 family member f; histone cluster 1 H3 family member g; histone cluster 1 H3 family member h; histone cluster 1 H3 family member i; histone cluster 1 H3 family member j; Histone cluster 1, H3a; Histone cluster 1, H3b; Histone cluster 1, H3c; Histone cluster 1, H3d; Histone cluster 1, H3e; Histone cluster 1, H3f; Histone cluster 1, H3g; Histone cluster 1, H3i; Histone cluster 1, H3j; Histone gene cluster 1, H3 histone family, member A; Histone gene cluster 1, H3 histone family, member B; Histone gene cluster 1, H3 histone family, member C; Histone gene cluster 1, H3 histone family, member D; Histone gene cluster 1, H3 histone family, member F; Histone gene cluster 1, H3 histone family, member G; Histone gene cluster 1, H3 histone family, member H; Histone gene cluster 1, H3 histone family, member I; Histone gene cluster 1, H3 histone family, member J; Histone gene cluster 1, H3A; Histone gene cluster 1, H3B; Histone gene cluster 1, H3C; Histone gene cluster 1, H3D; Histone gene cluster 1, H3E; Histone gene cluster 1, H3F; Histone gene cluster 1, H3G; Histone gene cluster 1, H3H; Histone gene cluster 1, H3I; Histone gene cluster 1, H3J; Histone H 3; Histone H3.1; Histone H3.2; Histone H3.3; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m;

抗原和靶标

免疫原:

A synthetic methylated peptide derived from human Tri-Methyl-Histone H3 around the methylation site of Lys9.

基因/基因ID:
HIST3H3(8290)
描述:
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

研究领域

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

文献引用

1). Tissue-resident trained immunity in hepatocytes protects against septic liver injury in zebrafish. Cell reports, 2024 (PubMed: 38850536) [IF=7.5]
2). Bromoacetic acid impairs mouse oocyte in vitro maturation through affecting cytoskeleton architecture and epigenetic modification. Chemico-Biological Interactions, 2022 (PubMed: 36174739) [IF=4.7]
3). Bisphenol AF negatively affects oocyte maturation of mouse in vitro through increasing oxidative stress and DNA damage. Chemico-Biological Interactions, 2017 (PubMed: 29102535) [IF=4.7]
4). Bisphenol B exposure disrupts mouse oocyte meiotic maturation in vitro through affecting spindle assembly and chromosome alignment. Frontiers in Cell and Developmental Biology, 2020 (PubMed: 33392205) [IF=4.6]

Application: IF/ICC    Species: mouse    Sample: oocytes

FIGURE 7 | BPB exposure altered epigenetic modification in mouse oocytes.(A) Images depicting H3K27me3 in control and BPB-treated oocytes.H3K27me3, red. Bar, 10 µm.(B) H3K27me3 fluorescence intensity in control and BPB-exposed oocytes. Control, n = 29; BPB, n = 31. ∗Significantly different (P < 0.05). (C) Images depicting H3K9me3 in control and BPB-treated oocytes. H3K9me3, red. Bar, 10 µm.
5). Poly(I:C) induces anti-inflammatory response against secondary LPS challenge in zebrafish larvae. Fish & shellfish immunology, 2024 (PubMed: 38092095) [IF=4.1]
6). Mannan-oligosaccharide induces trained immunity activation and alleviates pathological liver injury in turbot (Scophthalmus maximus). Aquaculture, 2024 [IF=3.9]
7). WDR62 regulates mouse oocyte meiotic maturation related to p-JNK and H3K9 trimethylation. The International Journal of Biochemistry & Cell Biology, 2022 (PubMed: 35093571) [IF=3.4]
8). Effects of Acute Fluorene-9-Bisphenol Exposure on Mouse Oocyte in vitro Maturation and Its Possible Mechanisms. ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 2019 (PubMed: 30499614) [IF=2.3]

Application: IF/ICC    Species: mouse    Sample: oocytes

Fig. 6. |BHPF exposure alters H3K9me3 and H3K27me3 levels in mouse oocytes. (A) Immunofluorescent staining for H3K9me3 in control and BHPF-treated oocytes. H3K9me3 level was significantly higher in treated oocytes. Red, H3K9me3; Blue, DAPI. Scale bar, 5 μm.
9). Isobutylparaben negatively affects porcine oocyte maturation through increasing oxidative stress and cytoskeletal abnormalities. ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 2020 (PubMed: 31922297) [IF=2.3]

Application: IF/ICC    Species: Pig    Sample: Cumulus cells

Fig. 6. IBP exposure alters H3K9me3 and H3K27me3 levels in porcine oocytes. (A) Immunofluorescent staining of H3K9me3 and H3K27me3 in control and IBP exposure group. The histone methylation level was significantly higher in treated oocytes. H3K9me3 and H3K27me3; Blue,DAPI. (B) Average H3K9me3 fluorescent intensity of H3K9me3 in control and IBP-treated oocytes. (C) Average fluorescent intensity of H3K27me3 in control and IBP-treated oocytes. **P < 0.01; ***P < 0.001; scale bar = 50 μm. DIC, differential interference contrast.
10). Hyperuricemia Facilitates Uric Acid-Mediated Vascular Endothelial Cell Damage by Inhibiting Mitophagy. Cell biochemistry and biophysics, 2025 (PubMed: 39340591) [IF=1.8]

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