Mono-Methyl-Histone H3 (Lys4)/H3K4me1 Antibody - #DF6933

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产品: Mono-Methyl-Histone H3 (Lys4)/H3K4me1 抗体
货号: DF6933
描述: Rabbit polyclonal antibody to Mono-Methyl-Histone H3 (Lys4)/H3K4me1
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Horse, Sheep, Rabbit
蛋白号: Q16695
RRID: AB_2838892

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 50ul RMB¥ 1250 现货
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说明书
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Mono-Methyl-Histone H3 (Lys4)/H3K4me1 Antibody detects endogenous levels of Mono-Methyl-Histone H3 only when methylated at Lys4.
RRID:
AB_2838892
引用格式: Affinity Biosciences Cat# DF6933, RRID:AB_2838892.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

;H3.3A; HIST1 cluster, H3E; H3 histone family, member A; H3.1; H3/l; H3F3; H3FF; H3FJ; H3FL; Histone gene cluster 1, H3 histone family, member E; histone H3.1t; Histone H3/o; FLJ92264; H 3; H3; H3 histone family, member B; H3 histone family, member C; H3 histone family, member D; H3 histone family, member F; H3 histone family, member H; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member T; H3 histone, family 3A; H3/A; H3/b; H3/c; H3/d; h3/f; H3/h; H3/i; H3/j; H3/k; H3/t; H31_HUMAN; H3F1K; H3F3A; H3FA; H3FB; H3FC; H3FD; H3FH; H3FI; H3FK; HIST1 cluster, H3A; HIST1 cluster, H3B; HIST1 cluster, H3C; HIST1 cluster, H3D; HIST1 cluster, H3F; HIST1 cluster, H3G; HIST1 cluster, H3H; HIST1 cluster, H3I; HIST1 cluster, H3J; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; Histone 1, H3a; Histone 1, H3b; Histone 1, H3c; Histone 1, H3d; Histone 1, H3e; Histone 1, H3f; Histone 1, H3g; Histone 1, H3h; Histone 1, H3i; Histone 3, H3; histone cluster 1 H3 family member a; histone cluster 1 H3 family member b; histone cluster 1 H3 family member c; histone cluster 1 H3 family member d; histone cluster 1 H3 family member e; histone cluster 1 H3 family member f; histone cluster 1 H3 family member g; histone cluster 1 H3 family member h; histone cluster 1 H3 family member i; histone cluster 1 H3 family member j; Histone cluster 1, H3a; Histone cluster 1, H3b; Histone cluster 1, H3c; Histone cluster 1, H3d; Histone cluster 1, H3e; Histone cluster 1, H3f; Histone cluster 1, H3g; Histone cluster 1, H3i; Histone cluster 1, H3j; Histone gene cluster 1, H3 histone family, member A; Histone gene cluster 1, H3 histone family, member B; Histone gene cluster 1, H3 histone family, member C; Histone gene cluster 1, H3 histone family, member D; Histone gene cluster 1, H3 histone family, member F; Histone gene cluster 1, H3 histone family, member G; Histone gene cluster 1, H3 histone family, member H; Histone gene cluster 1, H3 histone family, member I; Histone gene cluster 1, H3 histone family, member J; Histone gene cluster 1, H3A; Histone gene cluster 1, H3B; Histone gene cluster 1, H3C; Histone gene cluster 1, H3D; Histone gene cluster 1, H3E; Histone gene cluster 1, H3F; Histone gene cluster 1, H3G; Histone gene cluster 1, H3H; Histone gene cluster 1, H3I; Histone gene cluster 1, H3J; Histone H 3; Histone H3.1; Histone H3.2; Histone H3.3; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m;

抗原和靶标

免疫原:

A synthetic methylated peptide derived from human Mono-Methyl-Histone H3 around the methylation site of Lys4.

基因/基因ID:
HIST3H3(8290)
描述:
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

研究领域

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Immune diseases > Systemic lupus erythematosus.

文献引用

1). Tissue-resident trained immunity in hepatocytes protects against septic liver injury in zebrafish. Cell reports, 2024 (PubMed: 38850536) [IF=7.5]
2). EPC1/2 regulate hematopoietic stem and progenitor cell proliferation by modulating H3 acetylation and DLST. iScience, 2024 (PubMed: 38439957) [IF=5.8]

Application: WB    Species: Human    Sample:

Fig S7. Effects of epc1a and epc2 deficiency on H3 or H4 acetylated protein, related to Figures 2 and 3. (A) Enriched GO terms for acetylation-related DEGs in epc1a-/- mutants. (B) The quantification of H3K9Ac (B1), H3K27Ac (B2) and H3K56Ac (B3) proteins in control, epc1a-/- and epc2-/- embryos. (C) H4K5/K8/K12/K16Ac protein level in the control, epc1a-/- and epc2-/- embryos at 33 hpf (C1), and quantification analysis (C2). (D, E) Western blotting analysis of H3K4me1 (D1) and H3K27me3 (E1) in the control, epc1a-/- and epc2-/- embryos, with Actin and PCNA as internal controls, and quantification of H3K4me1 (D2) and H3K27me3 (E2). (F, G) Double staining of runx1-GFP with anti-H3K9Ac (F1-F12) and H3K56Ac (G1-G12) in the CHT in the control, epc1a-/- and epc2-/- embryos at 72 hpf, and quantification of runx1+ cells (F13, G13), runx1+H3K9Ac+ (F14) and runx1+H3K56Ac+ cells (G14), with white arrowheads indicating double-positive cells. F4, F8, F12, G4, G8 and G12 show the magnified images of F3, F7, F11, G3, G7 and G11, respectively. (H) Protein level of H3K27Ac in the control, epc1a-/- and epc2-/- embryos and the corresponding groups treated with VPA (H1), and quantification analysis (H2). Each experiment was repeated three times, and a representative result is shown. All embryos are shown in lateral view, anterior to the left, and dorsal to the up. Scale bars, 100 μm (F1-F12, G1-G12). Data are presented as mean ± SD (n ≥ 3). t-test, *P < 0.05, **P < 0.01, ***P < 0.001, NS, not significant.
3). Poly(I:C) induces anti-inflammatory response against secondary LPS challenge in zebrafish larvae. Fish & shellfish immunology, 2024 (PubMed: 38092095) [IF=4.1]
4). Mannan-oligosaccharide induces trained immunity activation and alleviates pathological liver injury in turbot (Scophthalmus maximus). Aquaculture, 2024 [IF=3.9]

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