USP7 Antibody - #DF6931

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产品: USP7 抗体
货号: DF6931
描述: Rabbit polyclonal antibody to USP7
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: Q93009
RRID: AB_2838890

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:20-1:50
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
USP7 Antibody detects endogenous levels of total USP7.
RRID:
AB_2838890
引用格式: Affinity Biosciences Cat# DF6931, RRID:AB_2838890.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Deubiquitinating enzyme 7; HAUSP; Herpes virus associated ubiquitin specific protease; Herpesvirus-associated ubiquitin-specific protease; TEF 1; tef-1; TEF1; Ubiquitin carboxyl terminal hydrolase 7; Ubiquitin carboxyl-terminal hydrolase 7; Ubiquitin specific peptidase 7 (herpes virus associated); Ubiquitin specific peptidase 7; Ubiquitin specific peptidase 7 herpes virus associated; Ubiquitin specific processing protease 7; Ubiquitin specific protease 7 (herpes virus associated); Ubiquitin specific protease 7; Ubiquitin specific protease 7 herpes virus associated; Ubiquitin thioesterase 7; Ubiquitin thiolesterase 7; Ubiquitin-specific-processing protease 7; UBP 7; UBP-7; UBP7; UBP7_HUMAN; USP 7; usp-7; Usp7; VMW110-ASSOCIATED PROTEIN, 135-KD;

抗原和靶标

免疫原:

A synthesized peptide derived from human USP7, corresponding to a region within the internal amino acids.

基因/基因ID:
USP7(7874)
描述:
Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD and JAMM enzymes. Herpesvirus-associated ubiquitin-specific protease (HAUSP, USP7) is an important deubiquitinase belonging to USP subfamily. A key HAUSP function is to bind and deubiquitinate the p53 transcription factor and an associated regulator protein Mdm2, thereby stabilizing both proteins (3,4). In addition to regulating essential components of the p53 pathway, HAUSP also modifies other ubiquitinylated proteins such as members of the FoxO family of forkhead transcription factors and the mitotic stress checkpoint protein CHFR (5,6).

研究领域

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

文献引用

1). Ubiquitin-specific protease 7 regulates macrophage polarization via pyruvate kinase M2-mediated metabolic reprogramming in severe acute pancreatitis. Cell death & disease, 2025 (PubMed: 41145464) [IF=8.1]

Application: WB    Species: Mouse    Sample:

Fig. 1: USP7 was highly expressed in the pancreatic macrophages of SAP mice. A Serum amylase and lipase activities from the ELISA. B Pancreatic tissue damage according to the HE staining. C USP7 expression in pancreatic tissues based on the IHC staining. D Representation of USP7 protein expression from the results of Western blot from three independent experiments. E CD68 and USP7 were expressed in similar positions in pancreatic tissues according to immunofluorescence. n = 3 for (D), n = 6 for (A–C, E).
2). USP7-mediated stabilization of CXCL3 aggravates inflammation in models of acute lung injury. Journal of thoracic disease, 2025 (PubMed: 41158338) [IF=2.1]

Application: WB    Species: human    Sample: CXCL3 and USP7

Figure 4. USP7 regulated CXCL3 via deubiquitination. (A) qRT-PCR analyzed LPS-induced changes in USP7 and USP14 mRNA levels. (B) Western blots showed how USP7 and USP14 knockdown altered CXCL3 protein expression. (C) qRT-PCR probed USP7 depletion’s impact on CXCL3 mRNA. (D) Co-IP assays illuminated interactions between CXCL3 and USP7. (E) Western blots validated USP7 knockdown and overexpression in HLMVECs. (F,G) Actinomycin D tests assessed USP7’s role in CXCL3 mRNA stability. (H) After CHX exposure, USP7 overexpression’s effect on CXCL3 protein was measured by Western blot. (I) Western blots, post-treatment with si-NC, si-USP7, or si-USP7 + MG132, evaluated CXCL3 protein levels. ns, no statistically significant difference; **, P

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