Claudin 1 Antibody - #DF6919

>>买3送1 已结束>>
(4)   (4)  
产品: Claudin 1 抗体
货号: DF6919
描述: Rabbit polyclonal antibody to Claudin 1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Chicken
分子量: 23kDa; 23kD(Calculated).
蛋白号: O95832
RRID: AB_2838878

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(83%)
克隆:
Polyclonal
特异性:
Claudin 1 Antibody detects endogenous levels of total Claudin 1.
RRID:
AB_2838878
引用格式: Affinity Biosciences Cat# DF6919, RRID:AB_2838878.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Claudin-1; Claudin1; CLD 1; CLD1; CLD1_HUMAN; CLDN 1; Cldn1; ILVASC; SEMP 1; SEMP1; Senescence associated epithelial membrane protein 1; Senescence associated epithelial membrane protein; Senescence-associated epithelial membrane protein;

抗原和靶标

免疫原:
Uniprot:

O95832(CLD1_HUMAN) >>Visit HPA database.

基因/基因ID:
CLDN1(9076)
表达:
O95832 CLD1_HUMAN:

Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.

描述:
Tight junctions, or zonula occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces. Tight junctions are composed of claudin and occludin proteins, which join the junctions to the cytoskeleton (1,2). The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). Claudin-1 is expressed primarily in keratinocytes (4) and normal mammary epithelial cells, but is absent or reduced in breast carcinomas and breast cancer cell lines (5,6).
序列:
MANAGLQLLGFILAFLGWIGAIVSTALPQWRIYSYAGDNIVTAQAMYEGLWMSCVSQSTGQIQCKVFDSLLNLSSTLQATRALMVVGILLGVIAIFVATVGMKCMKCLEDDEVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Chicken
83
Dog
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O95832 作为底物

Site PTM Type Enzyme
K115 Ubiquitination
T153 Phosphorylation
K189 Ubiquitination
T191 O-Glycosylation
T191 Phosphorylation
S192 O-Glycosylation
S192 Phosphorylation
T195 Phosphorylation
K201 Ubiquitination
S205 O-Glycosylation
S205 Phosphorylation
S206 O-Glycosylation
S206 Phosphorylation
K208 Ubiquitination
Y210 Phosphorylation

研究背景

功能:

Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions.

(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes. Associates with CD81 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell. Acts as a receptor for dengue virus.

细胞定位:

Cell junction>Tight junction. Cell membrane>Multi-pass membrane protein. Basolateral cell membrane.
Note: Associates with CD81 and the CLDN1-CD81 complex localizes to the basolateral cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.

亚基结构:

Homopolymers interact with CLDN3, but not CLDN2, homopolymers. Can form homo- and heteropolymers with other claudin family members. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ and PATJ (By similarity). Interacts with OCLN, CLDN4, CLDN6 and CLDN9. Interacts with CD81.

(Microbial infection) Interacts with hepatitis c virus E1 and E2 proteins.

(Microbial infection) Interacts with dengue virus small envelope protein M.

蛋白家族:

Belongs to the claudin family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

文献引用

1). Capsaicin shapes gut microbiota and pre-metastatic niche to facilitate cancer metastasis to liver. Pharmacological Research (PubMed: 36608780) [IF=9.3]
2). Polysaccharide from Strongylocentrotus nudus eggs regulates intestinal epithelial autophagy through CD36/PI3K-Akt pathway to ameliorate inflammatory bowel disease. International Journal of Biological Macromolecules (PubMed: 37327932) [IF=8.2]
3). Hypoxia activates SUMO-1-HIF-1α signaling pathway to upregulate pro-inflammatory cytokines and permeability in human tonsil epithelial cells. LIFE SCIENCES (PubMed: 33794253) [IF=6.1]

Application: IHC    Species: Human    Sample: HTEC cells

Fig. 3. SUMO1 silencing contributes to reduction in permeability of HTEC cells under hypoxic condition. (A). Knockdown of SUMO1 inhibited the secretion of IL-6, IL-8 and TNF-α in HTEC cells treated under hypoxic condition for 24 h. ***p < 0.001 vs control, !!p < 0.01 vs siNC. (B). SUMO1 silencing promoted the TEER value in HTEC cells treated under hypoxic condition for 24 h. ***p < 0.001 vs control, !!!p < 0.001 vs siNC. (C). SUMO-1 silencing reduced the permeability of HTEC cells treated under hypoxic condition for 24 h. *p < 0.05 vs control, ***p < 0.001 vs siNC; !!!p < 0.001 vs siNC. (D). Western blotting was used to examine the protein levels of SUMO1, HIF-1α, VEGF, Occluding, Claudin-1 and ZO-1 in cells as indicated. (E). Immunohistochemistry staining assay was performed to determine the levels of ZO-1, Occluding and Claudin-1 in cells as indicated above.

Application: WB    Species: Human    Sample: HTEC cells

Fig. 3. SUMO1 silencing contributes to reduction in permeability of HTEC cells under hypoxic condition. (A). Knockdown of SUMO1 inhibited the secretion of IL-6, IL-8 and TNF-α in HTEC cells treated under hypoxic condition for 24 h. ***p < 0.001 vs control, !!p < 0.01 vs siNC. (B). SUMO1 silencing promoted the TEER value in HTEC cells treated under hypoxic condition for 24 h. ***p < 0.001 vs control, !!!p < 0.001 vs siNC. (C). SUMO-1 silencing reduced the permeability of HTEC cells treated under hypoxic condition for 24 h. *p < 0.05 vs control, ***p < 0.001 vs siNC; !!!p < 0.001 vs siNC. (D). Western blotting was used to examine the protein levels of SUMO1, HIF-1α, VEGF, Occluding, Claudin-1 and ZO-1 in cells as indicated. (E). Immunohistochemistry staining assay was performed to determine the levels of ZO-1, Occluding and Claudin-1 in cells as indicated above.
4). Longan Polysaccharides with Covalent Selenylation Combat the Fumonisin B1-Induced Cell Toxicity and Barrier Disruption in Intestinal Epithelial (IEC-6) Cells. Nutrients (PubMed: 37960333) [IF=5.9]

Application: WB    Species: Rat    Sample: IEC-6 cells

Figure 7 Expression of ZO-1, occludin, and claudin-1 in IEC-6 cells treated by the medium or polysaccharide products (LP, SeLP1, and SeLP2, 10 μg/mL) for 24 h, followed by 40 μmol/L FB1 exposure for 48 h.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.