| 产品: | ITGB2/CD18 抗体 |
| 货号: | DF6896 |
| 描述: | Rabbit polyclonal antibody to ITGB2/CD18 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig |
| 分子量: | 85kDa; 85kD(Calculated). |
| 蛋白号: | P05107 |
| RRID: | AB_2838855 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6896, RRID:AB_2838855.
展开/折叠
95 subunit beta; Beta 2 (antigen CD18 (p95); CD 18; CD18; Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor); Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta; Cell surface adhesion glycoproteins LFA-1/CR3/p150; Complement component 3 receptor 3 and 4 subunit; Complement receptor C3 beta subunit; Complement receptor C3 subunit beta; Integrin beta 2; Integrin beta chain beta 2; Integrin beta-2 (ITGB2); Integrin beta-2; Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); ITB2_HUMAN; ITGB2; LAD; LCAMB; Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C; Leukocyte cell adhesion molecule CD18; LFA 1; LFA1; Lymphocyte function associated antigen 1 (LFA1); Lymphocyte function associated antigen 1; MAC 1; MAC1; Macrophage antigen 1 (mac1) beta; MF17; MFI7; OTTHUMP00000115278; OTTHUMP00000115279; OTTHUMP00000115280; OTTHUMP00000115281; OTTHUMP00000115282;
抗原和靶标
Leukocytes (PubMed:23775590). Expressed in neutrophils (at protein level) (PubMed:21193407, PubMed:28807980).
- P05107 ITB2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLGLRPPLLALVGLLSLGCVLSQECTKFKVSSCRECIESGPGCTWCQKLNFTGPGDPDSIRCDTRPQLLMRGCAADDIMDPTSLAETQEDHNGGQKQLSPQKVTLYLRPGQAAAFNVTFRRAKGYPIDLYYLMDLSYSMLDDLRNVKKLGGDLLRALNEITESGRIGFGSFVDKTVLPFVNTHPDKLRNPCPNKEKECQPPFAFRHVLKLTNNSNQFQTEVGKQLISGNLDAPEGGLDAMMQVAACPEEIGWRNVTRLLVFATDDGFHFAGDGKLGAILTPNDGRCHLEDNLYKRSNEFDYPSVGQLAHKLAENNIQPIFAVTSRMVKTYEKLTEIIPKSAVGELSEDSSNVVQLIKNAYNKLSSRVFLDHNALPDTLKVTYDSFCSNGVTHRNQPRGDCDGVQINVPITFQVKVTATECIQEQSFVIRALGFTDIVTVQVLPQCECRCRDQSRDRSLCHGKGFLECGICRCDTGYIGKNCECQTQGRSSQELEGSCRKDNNSIICSGLGDCVCGQCLCHTSDVPGKLIYGQYCECDTINCERYNGQVCGGPGRGLCFCGKCRCHPGFEGSACQCERTTEGCLNPRRVECSGRGRCRCNVCECHSGYQLPLCQECPGCPSPCGKYISCAECLKFEKGPFGKNCSAACPGLQLSNNPVKGRTCKERDSEGCWVAYTLEQQDGMDRYLIYVDESRECVAGPNIAAIVGGTVAGIVLIGILLLVIWKALIHLSDLREYRRFEKEKLKSQWNNDNPLFKSATTTVMNPKFAES
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P05107 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K29 | Ubiquitination | Uniprot | |
| N50 | N-Glycosylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| N116 | N-Glycosylation | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K186 | Ubiquitination | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| N212 | N-Glycosylation | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| K332 | Ubiquitination | Uniprot | |
| K362 | Ubiquitination | Uniprot | |
| K379 | Ubiquitination | Uniprot | |
| K462 | Ubiquitination | Uniprot | |
| K479 | Ubiquitination | Uniprot | |
| K561 | Ubiquitination | Uniprot | |
| S591 | Phosphorylation | Uniprot | |
| K633 | Ubiquitination | Uniprot | |
| K636 | Ubiquitination | Uniprot | |
| K641 | Ubiquitination | Uniprot | |
| K658 | Ubiquitination | Uniprot | |
| Y735 | Phosphorylation | P06239 (LCK) | Uniprot |
| K740 | Acetylation | Uniprot | |
| K744 | Ubiquitination | Uniprot | |
| S745 | Phosphorylation | P05771-2 (PRKCB) , P24723 (PRKCH) , Q05655 (PRKCD) , P17252 (PRKCA) | Uniprot |
| K755 | Ubiquitination | Uniprot | |
| S756 | Phosphorylation | Uniprot | |
| T758 | Phosphorylation | P24723 (PRKCH) , P17252 (PRKCA) , Q05655 (PRKCD) , P05771 (PRKCB) | Uniprot |
| T759 | Phosphorylation | Uniprot | |
| T760 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| K765 | Ubiquitination | Uniprot |
研究背景
Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL. Integrins ITGAM/ITGB2 and ITGAX/ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX/ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM/ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM/ITGB2 is also a receptor for factor X. Integrin ITGAD/ITGB2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages. In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils.
Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
Cell membrane>Single-pass type I membrane protein. Membrane raft>Single-pass type I membrane protein.
Leukocytes. Expressed in neutrophils (at protein level).
Heterodimer of an alpha and a beta subunit. The ITGB2 beta subunit associates with the ITGAL, ITGAM, ITGAX or ITGAD alpha subunits. Found in a complex with CD177 and ITGAM/CD11b. Interacts with FGR (By similarity). Interacts with COPS5 and RANBP9. Interacts with FLNA (via filamin repeats 4, 9, 12, 17, 19, 21, and 23). Interacts with THBD.
Belongs to the integrin beta chain family.
研究领域
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Human Diseases > Infectious diseases: Bacterial > Legionellosis.
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Human Diseases > Infectious diseases: Parasitic > Malaria.
· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.
· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Immune diseases > Rheumatoid arthritis.
· Human Diseases > Cardiovascular diseases > Viral myocarditis.
· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.