NR1H3 Antibody - #DF6864

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产品: NR1H3 抗体
货号: DF6864
描述: Rabbit polyclonal antibody to NR1H3
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 50kDa; 50kD(Calculated).
蛋白号: Q13133
RRID: AB_2838823

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(0%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
NR1H3 Antibody detects endogenous levels of total NR1H3.
RRID:
AB_2838823
引用格式: Affinity Biosciences Cat# DF6864, RRID:AB_2838823.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Liver X receptor alpha; LXR a; LXRA; NR1H3; NR1H3_HUMAN; Nuclear receptor subfamily 1 group H member 3; Oxysterols receptor LXR alpha; Oxysterols receptor LXR-alpha; RLD 1; RLD1;

抗原和靶标

免疫原:
Uniprot:

Q13133(NR1H3_HUMAN) >>Visit HPA database.

基因/基因ID:
NR1H3(10062)
表达:
Q13133 NR1H3_HUMAN:

Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.

描述:
The protein encoded by this gene belongs to the NR1 subfamily of the nuclear receptor superfamily. The NR1 family members are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. This protein is highly expressed in visceral organs, including liver, kidney and intestine. It forms a heterodimer with retinoid X receptor (RXR), and regulates expression of target genes containing retinoid response elements. Studies in mice lacking this gene suggest that it may play an important role in the regulation of cholesterol homeostasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
序列:
MSLWLGAPVPDIPPDSAVELWKPGAQDASSQAQGGSSCILREEARMPHSAGGTAGVGLEAAEPTALLTRAEPPSEPTEIRPQKRKKGPAPKMLGNELCSVCGDKASGFHYNVLSCEGCKGFFRRSVIKGAHYICHSGGHCPMDTYMRRKCQECRLRKCRQAGMREECVLSEEQIRLKKLKRQEEEQAHATSLPPRASSPPQILPQLSPEQLGMIEKLVAAQQQCNRRSFSDRLRVTPWPMAPDPHSREARQQRFAHFTELAIVSVQEIVDFAKQLPGFLQLSREDQIALLKTSAIEVMLLETSRRYNPGSESITFLKDFSYNREDFAKAGLQVEFINPIFEFSRAMNELQLNDAEFALLIAISIFSADRPNVQDQLQVERLQHTYVEALHAYVSIHHPHDRLMFPRMLMKLVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWDVHE

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q13133 作为底物

Site PTM Type Enzyme
S198 Phosphorylation P68400 (CSNK2A1)
S246 Phosphorylation
Y306 Phosphorylation
S310 Phosphorylation
S312 Phosphorylation
T314 Phosphorylation
K328 Sumoylation
S413 Phosphorylation
K434 Sumoylation
K434 Ubiquitination

研究背景

功能:

Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES (By similarity). LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides (By similarity). Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism (By similarity).

翻译修饰:

Ubiquitinated leading to its degradation by the proteasome.

细胞定位:

Nucleus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.

亚基结构:

Heterodimer of NR1H3 and RXR (retinoic acid receptor). Interacts with CCAR2 (via N-terminus) in a ligand-independent manner. Interacts with SIRT1 and this interaction is inhibited by CCAR2. Interacts with GPS2.

蛋白家族:

Belongs to the nuclear hormone receptor family. NR1 subfamily.

研究领域

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

文献引用

1). Nobiletin Ameliorates Hepatic Lipid Deposition, Oxidative Stress, and Inflammation by Mechanisms That Involve the Nrf2/NF-κB Axis in Nonalcoholic Fatty Liver Disease. Journal of agricultural and food chemistry (PubMed: 38073108) [IF=6.1]
2). Berberine Promotes OATP1B1 Expression and Rosuvastatin Uptake by Inducing Nuclear Translocation of FXR and LXRα. Frontiers in Pharmacology (PubMed: 32292349) [IF=5.6]

Application: WB    Species: human    Sample: HepG2 cells

FIGURE 5 | Silencing hFXR and hLXRa expression abolished berberine-induced OATP1B1 expression in HepG2 cells. HepG2 cells were transiently transfected with small interfering RNA (siRNA)-hFXR or siRNA-hLXRa, and 48 h later protein levels of FXR (A) and LXRa (C) were examined by Western blot. HepG2 cells were then treated with berberine (25 mM), GW4064, or GW3965 (10 mM) for 24 h, OATP1B1 protein levels were analyzed by Western blot (B, D).
3). Guang Chen Pi (the pericarp of Citrus reticulata Blanco's cultivars 'Chachi') inhibits macrophage-derived foam cell formation. JOURNAL OF ETHNOPHARMACOLOGY (PubMed: 35489660) [IF=5.4]
4). PPARα Agonist Exerts Protective Effects in Podocyte Injury via Inhibition of the ANGPTL3 Pathway. EXPERIMENTAL CELL RESEARCH (PubMed: 34499887) [IF=3.7]

Application: WB    Species: Mouse    Sample: podocytes

Fig. 1. Expression of ANGPTL3, LXR α , RXR α , and PPAR α in normal and PAN-treated mouse podocytes. (A) Representative gel image for Lxr, Rxr, Ppar α , Hnf, Pparβ, and Pparγ mRNA expression in unstimulated podocytes (MPC5). A 100 ng sample of RNA from MPC5 was reverse transcribed into single-stranded cDNA. The cDNA was used as a template for PCR. PCR products were resolved in a 1.2% agarose ethidium bromide gel and visualized with ultraviolet light. Real-time RT-PCR analysis (for mRNA expression) (B), western blotting (C), and quantification (D) of ANGPTL3, LXR α , RXR α , and PPAR α in the podocytes after treatment with PAN for 0, 6, 12, 18, and 24 h *P < 0.05, **P < 0.01 vs. 0 h in the same groups.
5). Three polymethoxyflavones from the peel of Citrus reticulata “Chachi” inhibits oxidized low-density lipoprotein-induced macrophage-derived foam cell formation. Frontiers in Cardiovascular Medicine (PubMed: 35966555) [IF=3.6]
6). Calcyclin-binding protein contributes to cholangiocarcinoma progression by inhibiting ubiquitination of MCM2. Oncology Research (PubMed: 37305391) [IF=3.1]

Application: WB    Species: Mouse    Sample:

Figure 4 Calcyclin-binding protein (CACYBP) knockdown facilitated degradation of minichromosome maintenance complex component 2 (MCM2) by inducing ubiquitination. (A) Relative mRNA levels of the top eleven differentially expressed genes (DEGs) in HUCCT1 cells after CACYBP silencing as determined by qPCR analysis. (B) Relative protein levels of MCM2, NR1H3, PRIM1, CCND1 and CCNG1 in HUCCT1 cells after CACYBP silencing as determined by western blot assays. (C) Viability of HUCCT1 cells after knockdown of NR1H3, PRIM1, CCND1, CCNG1 and MCM2 as assessed by Celigo cell count assays at indicated times. (D) Relative protein level of MCM2 in CACYBP-knockdown HUCCT1 cells treated with cycloheximide (50 mg/mL). (E) Relative protein level of MCM2 in CACYBP-knockdown HUCCT1 cells exposed to 10 μmol/L proteasome inhibitor MG-132 treatment. (F) Ubiquitination of MCM2 immunoprecipitated by anti-MCM2 antibody. Data are represented by mean ± standard deviation. (G) Detection of K48 and K63 mediated ubiquitination of MCM2 in HUCCT1 cells. *p < 0.05, **p < 0.01, ***p < 0.001.

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