EIF5A1 Antibody - #DF6754

>>买2送1 进行中>>
>>赠JD卡 进行中>>
(1)   (5)  
产品: EIF5A1 抗体
货号: DF6754
描述: Rabbit polyclonal antibody to EIF5A1
应用: WB IHC IF/ICC
文献验证: IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 16kDa; 17kD(Calculated).
蛋白号: P63241
RRID: AB_2838716

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Zebrafish(%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%)
克隆:
Polyclonal
特异性:
EIF5A Antibody detects endogenous levels of total EIF5A1.
RRID:
AB_2838716
引用格式: Affinity Biosciences Cat# DF6754, RRID:AB_2838716.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

eIF 4D; eIF 5A 1; EIF 5A; eIF 5A1; eIF-4D; eIF-5A; eIF-5A-1; eIF-5A1; eIF4D; eif5a; EIF5A1; eIF5AI; Eukaryotic initiation factor 5A; Eukaryotic initiation factor 5A isoform 1; Eukaryotic translation initiation factor 5A 1; Eukaryotic translation initiation factor 5A; Eukaryotic translation initiation factor 5A-1; IF5A1_HUMAN; MGC104255; MGC99547; Rev binding factor; Rev-binding factor; uORF A; uORF;

抗原和靶标

免疫原:
Uniprot:

P63241(IF5A1_HUMAN) >>Visit HPA database.

基因/基因ID:
EIF5A(1984)
表达:
P63241 IF5A1_HUMAN:

Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).

描述:
mRNA-binding protein involved in translation elongation. Has an important function at the level of Mrna turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.
序列:
MADDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIKAMAK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
80
Chicken
80
Xenopus
70
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.

翻译修饰:

Acetylated. Deacetylated by SIRT2.

eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).

细胞定位:

Cytoplasm. Nucleus. Endoplasmic reticulum membrane>Peripheral membrane protein>Cytoplasmic side. Nucleus>Nuclear pore complex.
Note: Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).

亚基结构:

Interacts with DHPS, with SDCBP and DOHH. Interacts with HIV-1 protein Rev. Found in a complex with Ran and XPO4. The hypusine modification increases the interaction with XPO4.

蛋白家族:

Belongs to the eIF-5A family.

文献引用

1). Comparison of the major cell populations among osteoarthritis, Kashin–Beck disease and healthy chondrocytes by single-cell RNA-seq analysis. Cell Death & Disease, 2021 (PubMed: 34045450) [IF=8.1]

Application: IHC    Species: Human    Sample: healthy cartilage tissues

Fig. 1 Single-cell RNA-sequencing analysis of human healthy control chondrocyte tissue samples. A Seven healthy human chondrocyte clusters. Visualisation of clustering by t-SNE plot of healthy control samples, identified by cell type. B Monocle pseudotime trajectory showing the progression of seven clusters of healthy chondrocytes. C Heat map of scaled gene expression data for the top 10 differentially expressed genes identifying each cluster, with selected genes listed. D Gene expression plots demonstrating high expression of SH3BGRL3 in SH3BGRL3hi fibrocartilage chondrocytes-1, IGFBP5 in IGFBP5hi fibrocartilage chondrocytes-2, CDC20 in CDC20hi cartilage progenitor cells-1, KIAA0101 in KIAA0101hi cartilage progenitor cells-2, EIF5A in EIF5Ahi regulatory chondrocytes, SOX9 in SOX9hi prehypertrophic chondrocytes and TXNIP in TXNIPhi homeostatic chondrocytes. Dot colour corresponds to the level of gene expression in each cell. t-SNE, t-distributed stochastic neighbour embedding. E Representative immunohistochemistry staining of SH3BGRL3, IGFBP5, BIRC5, SOX9, EIF5A and CDC20 in healthy cartilage tissues. Scale bar, left, 500 μm; right, 50 μm, and quantification of positive cells of different areas (superficial, middle, deep) in cartilage tissues displayed by box plot (n = 5). *p < 0.05.

Application: IHC    Species: human    Sample: cartilage

Fig. 1 |Single-cell RNA-sequencing analysis of human healthy control chondrocyte tissue samples.E Representative immunohistochemistry staining of SH3BGRL3, IGFBP5, BIRC5, SOX9, EIF5A and CDC20 in healthy cartilage tissues. Scale bar, left, 500 μm; right, 50 μm, and quantification of positive cells of different areas (superficial, middle, deep) in cartilage tissues displayed by box plot (n = 5). *p < 0.05.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.