ANGPTL4 Antibody - #DF6751

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产品: ANGPTL4 抗体
货号: DF6751
描述: Rabbit polyclonal antibody to ANGPTL4
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat
蛋白号: Q9BY76
RRID: AB_2838713

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
ANGPTL4 Antibody detects endogenous levels of total ANGPTL4.
RRID:
AB_2838713
引用格式: Affinity Biosciences Cat# DF6751, RRID:AB_2838713.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Angiopoietin like 4; Angiopoietin related protein 4; Angiopoietin-like protein 4; Angiopoietin-related protein 4; ANGL4_HUMAN; ANGPT L2; ANGPT L4; ANGPTL2; Angptl4; ARP4; Fasting induced adipose factor; FIAF; HARP; Hepatic angiopoietin related protein; Hepatic fibrinogen/angiopoietin related protein; Hepatic fibrinogen/angiopoietin-related protein; HFARP; NL2; Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein; PGAR; pp1158; PPARG angiopoietin related protein; PSEC0166; TGQTL; UNQ171; Weakly similar to angiopoietin 1 [H.sapiens];

抗原和靶标

免疫原:

A synthesized peptide derived from human ANGPTL4, corresponding to a region within N-terminal amino acids.

基因/基因ID:
ANGPTL4(51129)
描述:
This gene encodes a glycosylated, secreted protein containing a C-terminal fibrinogen domain. The encoded protein is induced by peroxisome proliferation activators and functions as a serum hormone that regulates glucose homeostasis, lipid metabolism, and insulin sensitivity. This protein can also act as an apoptosis survival factor for vascular endothelial cells and can prevent metastasis by inhibiting vascular growth and tumor cell invasion. The C-terminal domain may be proteolytically-cleaved from the full-length secreted protein. Decreased expression of this gene has been associated with type 2 diabetes. Alternative splicing results in multiple transcript variants. This gene was previously referred to as ANGPTL2 but has been renamed ANGPTL4.

研究领域

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Digestive system > Cholesterol metabolism.

文献引用

1). The ANGPTL4-HIF-1α loop: a critical regulator of renal interstitial fibrosis. Journal of translational medicine, 2024 (PubMed: 38992710) [IF=7.4]

Application: IHC    Species: Rat    Sample:

Fig. 2 Increased expression of HIF-1α and ANGPTL4 in CKD rats. A Immunohistochemical staining to assess the expression of HIF-1α, ANGPTL4, α-SMA, and Col-I in the renal tissues of the two groups (× 200 magnification, scale bar = 50 µm). B–E Quantification of immunohistochemical staining (n = 5/group). F–I qRT‒PCR analysis of the expression of Hif-1α, Angptl4, α-sma, and Col-I mRNA in the renal tissues of the two groups of rats. All the data are presented as the mean ± standard deviation; *P 

Application: WB    Species: human    Sample: HK2 cells

Fig. 5 The HIF-1α-ANGPTL4 pathway is activated in hypoxia-induced HK2 cell fibrosis. After 24 h of hypoxia induction in HK2 cells, an in vivo model of RIF induction was established. A Western blot analysis was conducted to assess the protein levels of HIF-1α, ANGPTL4, α-SMA, and Col-I, and the protein levels were quantified using ImageJ software (n = 3/group) (B–E). F–I qRT‒PCR was used to measure the mRNA expression of HIF-1α, ANGPTL4, α-SMA, and COL-I in each group. J–L Immunofluorescence staining for HIF-1α and ANGPTL4 (× 200 magnification, scale bar = 100 µm). All the data are presented as the mean ± standard deviation. *P 
2). Identification of Novel Organo-Se BTSA-Based Derivatives as Potent, Reversible, and Selective PPARγ Covalent Modulators for Antidiabetic Drug Discovery. Journal of medicinal chemistry, 2025 (PubMed: 39705161) [IF=6.8]
3). Salidroside inhibits osteoclast differentiation based on osteoblast-osteoclast interaction via HIF-1a pathway. Chinese journal of natural medicines, 2025 (PubMed: 40383613) [IF=4.0]
4). RNA sequencing reveals the potential mechanism of exercise preconditioning for cerebral ischemia reperfusion injury in rats. Brain and behavior, 2024 (PubMed: 38956886) [IF=2.6]

Application: WB    Species: Rat    Sample:

FIGURE 7 Exercise preconditioning (EP) suppressed TIMP1, SOCS3, ANGPTL4, CDO1, and SERPINE1 expressions of the cerebral cortices in middle cerebral artery occlusion (MCAO) rats. At 48 h after cerebral ischemia reperfusion injury (CIRI), TIMP1, SOCS3, ANGPTL4, CDO1, and SERPINE1 expression levels of cerebral cortices in each group were evaluated by qPCR (a) and Western blotting (b). # p < .05, ## p < .01 versus Sham. *p < .05, **p < .01 versus EP. Results were presented as mean ± SD. n = 3.
5). Danlou Tablet May Alleviate Vascular Injury Caused by Chronic Intermittent Hypoxia through Regulating FIH-1, HIF-1, and Angptl4. Evidence-based Complementary and Alternative Medicine, 2022 (PubMed: 36285165)

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