BMPR1B Antibody | Affinity Biosciences LTD|亲科生物官网

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$Figure 3 Effects of H10 on 17β-HSD3 in LC540 (17β-HSD3) cells. Effects of H10 on the production of (A) T and (B) P. (C) The expression of 17β-HSD3 mRNA. (D) The expression levels of the proteins in the T synthesis pathway were detected by western blotting following treatment with H10. The concentrations of H10 were 0.25, 0.5, and 1 μM, while the concentration of 3858858 (positive control) was 0.25 μM. (E) T produced from the microsomal fraction of rat testes containing 17β-HSD3. The data are reported as the mean ± sd (n = 3) of results obtained from individual experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 are considered to be significant, vs. the control.$

BMPR1B Antibody - Figure 3 Effects of H10 on 17β-HSD3 in LC540 (17β-HSD3) cells.

BMPR1B Antibody - FIGURE 4 | Downregulation of lncRNA SNHG6 regulates miR-125b-5p/BMPR1B signaling pathway in TNBC cells.

产品:	BMPR1B 抗体
货号:	DF6748
描述:	Rabbit polyclonal antibody to BMPR1B
应用:	WB IHC
文献验证:	WB
反应:	Human, Mouse
预测:	Pig, Bovine, Horse, Rabbit, Dog, Chicken
分子量:	57kDa; 57kD(Calculated).
蛋白号:	O00238
RRID:	AB_2838710

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1250	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

联系销售

MS Report

HPLC Report

MSDS

说明书

COA

实验方案

WB Handbook

IHC Protocol

IF/ICC Protocol

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse

预测:

Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%)

克隆:

Polyclonal

特异性:

BMPR1B Antibody detects endogenous levels of total BMPR1B.

RRID:

AB_2838710
引用格式: Affinity Biosciences Cat# DF6748, RRID:AB_2838710.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

Activin receptor like kinase 6; Acvrlk6; ALK 6; ALK6; alk6tr; BMP type-1B receptor; BMPR IB; BMPR-1B; Bmpr1b; BMPRIB; BMR1B_HUMAN; Bone morphogenetic protein receptor type 1B; Bone morphogenetic protein receptor type IB; Bone morphogenetic protein receptor type-1B; BR 1b; BR1b; CDw 293; CDw293; CDw293 antigen; CFK 43a; CFK43a; Serine/threonine receptor kinase; zALK 6; zALK6;

抗原和靶标

免疫原:

Uniprot:

O00238(BMR1B_HUMAN) >>Visit HPA database.

基因/基因ID:

BMPR1B(658)

描述:

This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

序列:

O00238 BMR1B_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLLRSAGKLNVGTKKEDGESTAPTPRPKVLRCKCHHHCPEDSVNNICSTDGYCFTMIEEDDSGLPVVTSGCLGLEGSDFQCRDTPIPHQRRSIECCTERNECNKDLHPTLPPLKNRDFVDGPIHHRALLISVTVCSLLLVLIILFCYFRYKRQETRPRYSIGLEQDETYIPPGESLRDLIEQSQSSGSGSGLPLLVQRTIAKQIQMVKQIGKGRYGEVWMGKWRGEKVAVKVFFTTEEASWFRETEIYQTVLMRHENILGFIAADIKGTGSWTQLYLITDYHENGSLYDYLKSTTLDAKSMLKLAYSSVSGLCHLHTEIFSTQGKPAIAHRDLKSKNILVKKNGTCCIADLGLAVKFISDTNEVDIPPNTRVGTKRYMPPEVLDESLNRNHFQSYIMADMYSFGLILWEVARRCVSGGIVEEYQLPYHDLVPSDPSYEDMREIVCIKKLRPSFPNRWSSDECLRQMGKLMTECWAHNPASRLTALRVKKTLAKMSESQDIKL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Dog

100

Chicken

100

Rabbit

100

Sheep

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O00238 作为底物

O00238

Site	PTM Type	Source
	Phosphorylation	Uniprot
S92	Phosphorylation	Uniprot
T97	Phosphorylation	Uniprot
T109	O-Glycosylation	Uniprot
S175	Phosphorylation	Uniprot
Y290	Phosphorylation	Uniprot
S300	Phosphorylation	Uniprot
K336	Ubiquitination	Uniprot
S402	Phosphorylation	Uniprot
K493	Ubiquitination	Uniprot

翻译修饰 - O00238 作为激酶

O00238

Substrate	Site	Source
O95630-1 (STAMBP)	S2	Uniprot
O95630-1 (STAMBP)	S48	Uniprot
O95630-1 (STAMBP)	S243	Uniprot
O95630-1 (STAMBP)	S245	Uniprot
O95630-1 (STAMBP)	S247	Uniprot
Q15796 (SMAD2)	S464	Uniprot
Q15796 (SMAD2)	S465	Uniprot
Q15796 (SMAD2)	S467	Uniprot
Q15797 (SMAD1)	S462	Uniprot
Q15797 (SMAD1)	S463	Uniprot
Q15797 (SMAD1)	S465	Uniprot

研究背景

O00238_BMR1B_HUMAN

功能:

On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP7/OP-1 and GDF5. Positively regulates chondrocyte differentiation through GDF5 interaction.

细胞定位:

Cell membrane. Membrane>Single-pass type I membrane protein.

亚基结构:

Interacts with high affinity with GDF5; positively regulates chondrocyte differentiation.

蛋白家族:

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)

· Organismal Systems > Development > Axon guidance. (View pathway)

文献引用

1). LncRNA SNHG6/miR-125b-5p/BMPR1B Axis: A New Therapeutic Target for Triple-Negative Breast Cancer. Frontiers in Oncology, 2021 (PubMed: 34026654) [IF=3.5]

Application: WB Species: Human Sample: LC540 cells

Figure 3 Effects of H10 on 17β-HSD3 in LC540 (17β-HSD3) cells. Effects of H10 on the production of (A) T and (B) P. (C) The expression of 17β-HSD3 mRNA. (D) The expression levels of the proteins in the T synthesis pathway were detected by western blotting following treatment with H10. The concentrations of H10 were 0.25, 0.5, and 1 μM, while the concentration of 3858858 (positive control) was 0.25 μM. (E) T produced from the microsomal fraction of rat testes containing 17β-HSD3. The data are reported as the mean ± sd (n = 3) of results obtained from individual experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 are considered to be significant, vs. the control.

Application: WB Species: Sample: TNBC cells

FIGURE 4 | Downregulation of lncRNA SNHG6 regulates miR-125b-5p/BMPR1B signaling pathway in TNBC cells.(G) miR-125b-5p inhibition upregulated BMPR1B protein expression.

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BMPR1B Antibody - #DF6748