产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6730, RRID:AB_2838692.
展开/折叠
60S ribosomal protein L5; DBA6; L5; MSTP030; PPP1R135; protein phosphatase 1, regulatory subunit 135; Ribosomal protein L5; RL5; RL5_HUMAN; RP L5; RPL 5; RPL5; U21 RNA; U21RNA;
抗原和靶标
- P46777 RL5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGFVKVVKNKAYFKRYQVKFRRRREGKTDYYARKRLVIQDKNKYNTPKYRMIVRVTNRDIICQIAYARIEGDMIVCAAYAHELPKYGVKVGLTNYAAAYCTGLLLARRLLNRFGMDKIYEGQVEVTGDEYNVESIDGQPGAFTCYLDAGLARTTTGNKVFGALKGAVDGGLSIPHSTKRFPGYDSESKEFNAEVHRKHIMGQNVADYMRYLMEEDEDAYKKQFSQYIKNSVTPDMMEEMYKKAHAAIRENPVYEKKPKKEVKKKRWNRPKMSLAQKKDRVAQKKASFLRAQERAAES
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P46777 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| G2 | Acetylation | Uniprot | |
| K5 | Acetylation | Uniprot | |
| K5 | Methylation | Uniprot | |
| K5 | Ubiquitination | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| K27 | Acetylation | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| Y30 | Phosphorylation | Uniprot | |
| Y31 | Phosphorylation | Uniprot | |
| K41 | Acetylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| K43 | Acetylation | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K48 | Acetylation | Uniprot | |
| K48 | Ubiquitination | Uniprot | |
| Y66 | Phosphorylation | Uniprot | |
| C76 | S-Nitrosylation | Uniprot | |
| C100 | S-Nitrosylation | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| K164 | Methylation | Uniprot | |
| K164 | Sumoylation | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| K178 | Acetylation | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| Y183 | Phosphorylation | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| K188 | Sumoylation | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| K197 | Acetylation | Uniprot | |
| K197 | Sumoylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| Y207 | Phosphorylation | Uniprot | |
| R209 | Methylation | Uniprot | |
| Y219 | Phosphorylation | Uniprot | |
| K220 | Acetylation | Uniprot | |
| K220 | Sumoylation | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| K221 | Acetylation | Uniprot | |
| K221 | Sumoylation | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| K228 | Methylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| T232 | Phosphorylation | Uniprot | |
| Y240 | Phosphorylation | Uniprot | |
| K241 | Acetylation | Uniprot | |
| K242 | Acetylation | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| R248 | Methylation | Uniprot | |
| Y253 | Phosphorylation | Uniprot | |
| K270 | Ubiquitination | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| K276 | Acetylation | Uniprot | |
| K283 | Methylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| S286 | Phosphorylation | Uniprot |
研究背景
Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs. It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53.
Cytoplasm. Nucleus>Nucleolus.
Component of the large ribosomal subunit (LSU). Part of a LSU subcomplex, the 5S RNP which is composed of the 5S RNA, RPL5 and RPL11. Interacts with isoform 1 of NVL in an ATP-dependent manner. Interacts with RRP1B.
Belongs to the universal ribosomal protein uL18 family.
研究领域
· Genetic Information Processing > Translation > Ribosome.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.