PSMB5 Antibody - #DF6728

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产品: PSMB5 抗体
货号: DF6728
描述: Rabbit polyclonal antibody to PSMB5
应用: WB IHC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 28kDa, 20 kDa; 28kD(Calculated).
蛋白号: P28074
RRID: AB_2838690

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(92%)
克隆:
Polyclonal
特异性:
PSMB5 Antibody detects endogenous levels of total PSMB5.
RRID:
AB_2838690
引用格式: Affinity Biosciences Cat# DF6728, RRID:AB_2838690.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DKFZp459C139; EC 3.4.25.1; LMPX; Macropain epsilon chain; MB1; MGC104214; MGC118075; MGC134464; Multicatalytic endopeptidase complex epsilon chain; Proteasome (prosome, macropain) subunit, beta type, 5; Proteasome beta 5 subunit; Proteasome catalytic subunit 3; Proteasome chain 6; Proteasome epsilon chain; Proteasome subunit beta type-5; Proteasome subunit MB1; Proteasome subunit X; Proteasome subunit, beta type, 5; Proteasome subunit, beta-5; PSB5_HUMAN; PSMB5; PSX large multifunctional protease X; X;

抗原和靶标

免疫原:
Uniprot:

P28074(PSB5_HUMAN) >>Visit HPA database.

基因/基因ID:
PSMB5(5693)
描述:
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene.
序列:
MALASVLERPLPVNQRGFFGLGGRADLLDLGPGSLSDGLSLAAPGWGVPEEPGIEMLHGTTTLAFKFRHGVIVAADSRATAGAYIASQTVKKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYKGMGLSMGTMICGWDKRGPGLYYVDSEGNRISGATFSVGSGSVYAYGVMDRGYSYDLEVEQAYDLARRAIYQATYRDAYSGGAVNLYHVREDGWIRVSSDNVADLHEKYSGSTP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Chicken
67
Zebrafish
58
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P28074 作为底物

Site PTM Type Enzyme
S87 Phosphorylation
K91 Ubiquitination
K92 Ubiquitination
Y99 Phosphorylation
S139 Phosphorylation
K140 Ubiquitination
Y147 Phosphorylation
Y149 Phosphorylation
K165 Ubiquitination
Y171 Phosphorylation
S175 Phosphorylation
Y212 Phosphorylation
Y220 Phosphorylation
Y228 Phosphorylation
Y236 Phosphorylation
S247 Phosphorylation
S248 Phosphorylation
K257 Acetylation
K257 Ubiquitination
S261 Phosphorylation
T262 Phosphorylation

研究背景

功能:

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB5 displays a chymotrypsin-like activity.

细胞定位:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. Directly interacts with POMP. Interacts with ABCB1 and TAP1.

(Microbial infection) Interacts with HIV-1 TAT protein.

蛋白家族:

Belongs to the peptidase T1B family.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > Proteasome.

文献引用

1). TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress. Cell Death & Disease (PubMed: 33414446) [IF=9.0]

Application: WB    Species: human    Sample: Bel-7402 and SMMC7721 cells

Fig. 2| PSMB5 is a target of TRIB2 in the proteasome.e PSMB5 was critical for TRIB2. Ub was measured in the Bel-7402 and SMMC7721 cells with or without PSMB5 knocked out or compensatory PSMB5 expression, in the presence or absence of TRIB2 knockdown.

Application: IF/ICC    Species: human    Sample: Bel-7402 and SMMC7721 cells

Fig. s3|IntheabsenceofTRIB2,thelevelofUbwasincreasedbecausePSMB5waslessactive(Figs.1–3).

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