Claudin 5 Antibody - #AF0130

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产品: Claudin 5 抗体
货号: AF0130
描述: Rabbit polyclonal antibody to Claudin 5
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Rabbit
分子量: 23kDa; 23kD(Calculated).
蛋白号: O00501
RRID: AB_2833314

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Bovine(%), Rabbit(%)
克隆:
Polyclonal
特异性:
Claudin 5 Antibody detects endogenous levels of total Claudin 5.
RRID:
AB_2833314
引用格式: Affinity Biosciences Cat# AF0130, RRID:AB_2833314.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Androgen withdrawal and apoptosis induced protein RVP1 like; AWAL; BEC 1; BEC1; Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome); Claudin-5; Claudin5; CLD5_HUMAN; CLDN 5; Cldn5; CPETR L1; CPETRL 1; CPETRL1; TMDVCF; TMVCF; Transmembrane protein deleted in VCFS; Transmembrane protein deleted in velocardiofacial syndrome;

抗原和靶标

免疫原:
Uniprot:

O00501(CLD5_HUMAN) >>Visit HPA database.

基因/基因ID:
CLDN5(7122)
描述:
claudin 5 Plays a major role in tight junction-specific obliteration of the intercellular space. Belongs to the claudin family. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO- 3. Interacts with MPDZ.
序列:
MGSAALEILGLVLCLVGWGGLILACGLPMWQVTAFLDHNIVTAQTTWKGLWMSCVVQSTGHMQCKVYDSVLALSTEVQAARALTVSAVLLAFVALFVTLAGAQCTTCVAPGPAKARVALTGGVLYLFCGLLALVPLCWFANIVVREFYDPSVPVSQKYELGAALYIGWAATALLMVGGCLLCCGAWVCTGRPDLSFPVKYSAPRRPTATGDYDKKNYV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
89
Rabbit
89
Chicken
78
Xenopus
75
Horse
0
Sheep
0
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Plays a major role in tight junction-specific obliteration of the intercellular space.

细胞定位:

Cell junction>Tight junction. Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ (By similarity).

蛋白家族:

Belongs to the claudin family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

文献引用

1). Engagement of circular RNA HECW2 in the nonautophagic role of ATG5 implicated in the endothelial-mesenchymal transition. Autophagy, 2018 (PubMed: 29260931) [IF=14.6]

Application: WB    Species: human    Sample: HBMECs

Figure 3|Role of ATG5 in LPS-induced EndoMT. Scale bar: 5 μm. Mice were administered LPS (0.83 mg/kg) once per day for 7 d. n=6 animals/group. *P<0.05,**P<0.01, ***P<0.001 versus saline group using the Mann-Whitney test. Transfection of HBMECs with siRNA ATG5 significantly inhibited the decreases in the TJP expression levels and the increases in the mesenchymal cell marker expression .
2). Axl deficiency promotes the neuroinvasion of Japanese encephalitis virus by enhancing IL-1α production from pyroptotic macrophages. Journal of Virology, 2020 (PubMed: 32611752) [IF=4.0]

Application: IF/ICC    Species: mouse    Sample: brains

Fig.9. |Effects of IL-1α alone on BBB permeability and tight junction integrity.(D) IF staining of claudin-5, occludin, and ZO-1 (red), CD31 (microvascular endothelial cell marker, green), and DAPI (nucleus, blue), scale bar=20 μm.
3). Activation of Sigma-1 Receptor Enhanced Pericyte Survival via the Interplay Between Apoptosis and Autophagy: Implications for Blood-Brain Barrier Integrity in Stroke. Translational Stroke Research, 2020 (PubMed: 31290080) [IF=3.8]

Application: WB    Species: mouse    Sample: brain

Fig. 1| Knockdown of σ-1R aggravated cerebral ischemia-induced injury in pMCAO mice.h, i The decrease in TJP expression in the ipsilateral side of the brain observed at 24 h after pMCAO surgery was aggravated in σ-1R KO mice. TJP expression levels were determined via western blot analysis (h) and quantified through densitometric analysis.
4). The truncated AXIN1 isoform promotes hepatocellular carcinoma metastasis through SRSF9-mediated exon 9 skipping. Molecular and cellular biochemistry, 2025 (PubMed: 38748384) [IF=3.5]

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