Claudin 5 Antibody - #AF0130

Figure 3|Role of ATG5 in LPS-induced EndoMT. Scale bar: 5 μm. Mice were administered LPS (0.83 mg/kg) once per day for 7 d. n=6 animals/group. *P<0.05,**P<0.01, ***P<0.001 versus saline group using the Mann-Whitney test. Transfection of HBMECs with siRNA ATG5 significantly inhibited the decreases in the TJP expression levels and the increases in the mesenchymal cell marker expression .

Fig. 1. Myocardial dilation, myocyte atrophy and Cldn5 reduction in the left atrium of AF patients (A) Exemplar images of electrocardiogram (ECG) and apical four-chamber view of the heart in AF patient and non-AF control. Red box indicates left atrium (LA). (B) Representative western blot of Cldn5 in left appendages of AF patients compared to those of donors. Human lung tissue excised during lung cancer resection is used as positive control. (C) Quantitative analysis of the protein level of Cldn5 in AF and non-AF groups. * P < 0.05 vs. Non-AF; n = 4. (D)Exemplar left atrial appendage section stained with anti-Cldn5, CD31 and TOMM20 antibodies. Anti-Cldn5 primary antibody is Alexa Fluor 488 conjugated (Green) while CD31 and TOMM20 antibodies is visualized by Fluorescein donkey anti-rabbit IgG Alexa Fluor 594 (Red). DAPI is used to stain nucleus (Blue). Scale bar, 20 μm. (E) Quantification of immunofluorescent staining of Cldn5 in cardiomyocytes. * P < 0.05 vs. Non-AF; n = 4. (F) Exemplar left atrial appendage section stained with wheat germ agglutinin (WGA) for assessing cardiomyocyte's cross section. Scale bar, 20 μm. (G) Quantification of cross-section of cardiomyocytes. * P < 0.05 vs. Non-AF; n = 4. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Fig.9. |Effects of IL-1α alone on BBB permeability and tight junction integrity.(D) IF staining of claudin-5, occludin, and ZO-1 (red), CD31 (microvascular endothelial cell marker, green), and DAPI (nucleus, blue), scale bar=20 μm.

Fig. 1| Knockdown of σ-1R aggravated cerebral ischemia-induced injury in pMCAO mice.h, i The decrease in TJP expression in the ipsilateral side of the brain observed at 24 h after pMCAO surgery was aggravated in σ-1R KO mice. TJP expression levels were determined via western blot analysis (h) and quantified through densitometric analysis.