ABCG8 Antibody - #DF6673

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产品: ABCG8 抗体
货号: DF6673
描述: Rabbit polyclonal antibody to ABCG8
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
蛋白号: Q9H221
RRID: AB_2838635

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
ABCG8 Antibody detects endogenous levels of total ABCG8.
RRID:
AB_2838635
引用格式: Affinity Biosciences Cat# DF6673, RRID:AB_2838635.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ABCG8; ABCG8_HUMAN; ATP binding cassette sub family G (WHITE) member 8 (sterolin 2); ATP binding cassette sub family G member 8; ATP binding cassette, sub family G (WHITE), member 8; ATP-binding cassette sub-family G member 8; GBD4; MGC142217; Sterolin 2; Sterolin-2; STSL;

抗原和靶标

免疫原:

A synthesized peptide derived from human ABCG8, corresponding to a region within the internal amino acids.

基因/基因ID:
ABCG8(64241)
描述:
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions to exclude non-cholesterol sterol entry at the intestinal level, promote excretion of cholesterol and sterols into bile, and to facilitate transport of sterols back into the intestinal lumen. It is expressed in a tissue-specific manner in the liver, intestine, and gallbladder. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG5. Mutations in this gene may contribute to sterol accumulation and atherosclerosis, and have been observed in patients with sitosterolemia. [provided by RefSeq, Jul 2008]

研究领域

· Environmental Information Processing > Membrane transport > ABC transporters.

· Organismal Systems > Digestive system > Fat digestion and absorption.

· Organismal Systems > Digestive system > Cholesterol metabolism.

文献引用

1). Caveolin-1 mitigates the advancement of metabolic dysfunction-associated steatotic liver disease by reducing endoplasmic reticulum stress and pyroptosis through the restoration of cholesterol homeostasis. International journal of biological sciences, 2025 (PubMed: 39781461) [IF=8.2]
2). Ginsenosides From Panax ginseng Improves Hepatic Lipid Metabolism Disorders in HFD-Fed Rats by Regulating Gut Microbiota and Cholesterol Metabolism Signaling Pathways. Phytotherapy research : PTR, 2024 (PubMed: 39660634) [IF=6.1]
3). Astragalus polysaccharides ameliorate diet-induced gallstone formation by modulating synthesis of bile acids and the gut microbiota. Frontiers in Pharmacology, 2021 (PubMed: 34276384) [IF=5.6]

Application: WB    Species: Mice    Sample: liver

FIGURE 5 Expression of the genes involved in cholesterol transport and bile-acid synthesis in the liver. (A) mRNA expression of Cyp7a1, Cyp8b1, Cyp7b1, and Cyp27a1 (n = 8). (B) Protein expression (left panel) and quantification (middle and right panel) of Cyp7a1 and Cyp7b1 (n = 6). (C) mRNA expression of Fgfr4 and β-klotho (n = 8). (D) mRNA expression of Abcg5 and Abcg8 (n = 8). (E) Protein expression (left panel) and quantification (middle and right panel) of Abcg5 and Abcg8 (n = 6). Data are the mean ± SD. *p < 0.05, **p < 0.01. Chow, chow diet; LD, lithogenic diet; AH, high-dose APS.
4). Ganoderma lucidum polysaccharide ameliorates cholesterol gallstone formation by modulating cholesterol and bile acid metabolism in an FXR-dependent manner. Chinese medicine, 2024 (PubMed: 38268006) [IF=5.3]
5). Hif-1α expression targets the TMA/Fmo3/TMAO axis to participate in gallbladder cholesterol stone formation in individuals living in plateau regions. Biochimica et biophysica acta. Molecular basis of disease, 2024 (PubMed: 38657913) [IF=4.2]
6). Gypenosides Prevent and Dissolve Cholesterol Gallstones by Modulating the Homeostasis of Cholesterol and Bile Acids. Frontiers in Medicine, 2022 (PubMed: 35445045) [IF=3.9]

Application: WB    Species: Mice    Sample:

Figure 7 Hepatic expression of genes involved in cholesterol transport and synthesis. (A) Levels of expression of Abcg5 and Abcg8 mRNAs measured by qRT-PCR (left panel, n = 8) and RNA sequencing (RNA-seq) analyses (right panel, data shown as means). (B) Protein expression and quantification of Abcg5 and Abcg8 (n = 6). (C) Levels of expression of Lxrα, Hmgcr and Srebp2 mRNAs measured by qRT-PCR (left panel, n = 8) and RNA-seq analyses (right panel, data shown as means). Data are reported as means ± SEM. **P < 0.01.
7). Molecular mechanism study and potential active ingredients prediction of Danhe granules in the treatment of hypercholesterolemia via TICE. Peptide Science, 2024 [IF=1.5]
8). Identification and Analysis of Three Hub Prognostic Genes Related to Osteosarcoma Metastasis. Journal of Oncology, 2021 (PubMed: 33564309)

Application: IHC    Species: Rat    Sample: tumor tissues

Figure 14 Immunohistochemical staining results.

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