IKZF1 Antibody - #DF6659

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产品: IKZF1 抗体
货号: DF6659
描述: Rabbit polyclonal antibody to IKZF1
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Horse, Rabbit, Dog
蛋白号: Q13422
RRID: AB_2838621

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
IKZF1 Antibody detects endogenous levels of total IKZF1.
RRID:
AB_2838621
引用格式: Affinity Biosciences Cat# DF6659, RRID:AB_2838621.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CLL associated antigen KW 6; DNA-binding protein Ikaros; hIk 1; Hs.54452; IK1; Ikaros (zinc finger protein); IKAROS; IKAROS family zinc finger 1 (Ikaros); Ikaros family zinc finger protein 1; Ikzf1; IKZF1_HUMAN; LYF1; Lymphoid transcription factor LyF-1; PRO0758; Zinc finger protein subfamily 1A 1 (Ikaros); Zinc finger protein subfamily 1A 1; Zinc finger protein, subfamily 1A, member 1; ZNFN1A1;

抗原和靶标

免疫原:

A synthesized peptide derived from human IKZF1, corresponding to a region within the internal amino acids.

基因/基因ID:
IKZF1(10320)
描述:
Ikaros family proteins, characterized by the presence of an N-terminal zinc finger DNA-binding domain and a C-terminal dimerization domain, belong to the Kruppel transcription factor superfamily. The Ikaros family includes Ikaros, Aiolos, Helios, and possibly EOS and Pegasus (1). They can form homodimers and heterodimers with other members of the Ikaros family. Due to differential splicing, multiple isoforms can be generated and some behave in a dominant negative manner upon dimerization (2).Ikaros, also known as Ikaros family zinc finger protein 1 (IKZF1) and Lymphoid transcription factor 1 (LYF-1), is expressed abundantly in blood cells. Genetic studies in mice demonstrate that Ikaros is important for the normal development of B, T, natural killer, and dendritic cells, and that it functions as a tumor suppressor (3,4). Research studies have shown that imbalanced expression of different Ikaros isoforms, as well as deletions of Ikaros are associated with hematologic malignancies in humans (5-7).

文献引用

1). Chidamide-Induced Accumulation of Reactive Oxygen Species Increases Lenalidomide Sensitivity Against Multiple Myeloma Cells. OncoTargets and Therapy, 2021 (PubMed: 34262292) [IF=2.7]

Application: WB    Species: Human    Sample: ARP-1 and RPMI-8226 cells

Figure 5 Chidamide (CHI)-induced ROS accumulation enhances anti-myeloma effect of lenalidomide (Len) through elevating H2O2. (A and B) ARP-1 and RPMI-8226 cells were treated with different doses of lenalidomide (0, 5 and 20 μM) for indicated time (0, 3, 6 and 12 hours), pretreated with or without 15 mmol/L NAC. Then ROS levels were measured using Reactive Oxygen Species Assay Kit. NS, p > 0.05; *, p < 0.05; **, p < 0.01. (C and D) ARP-1 and RPMI-8226 cells were treated with different doses of lenalidomide (0, 5 and 20 μM) for 12 hours, or 1 μM chidamide and 20 μM lenalidomide for 6 hours. Then H2O2 levels were tested using hydrogen peroxide assay kit. (E and F) after treated with 1 μM chidamide, 20 μM lenalidomide or H2O2 (100 and 500 μM) for 6 hours, expression of IKZF1 and IKZF3 were determined using Western blotting in ARP-1 and RPMI-8226 cells. (G and H) pretreated with or without 15 mmol/L NAC, ARP-1 and RPMI-8226 cells were then treated with 1 μM chidamide, 20 μM lenalidomide or 20 μM H2O2 for 24 hours, and CCK-8 assays were used to evaluate the cell viability. *, p < 0.05; **, p < 0.01; ***, p < 0.001.

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