IL16 Antibody - #DF6600

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产品: IL16 抗体
货号: DF6600
描述: Rabbit polyclonal antibody to IL16
应用: WB IHC
文献验证: IHC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep
蛋白号: Q14005
RRID: AB_2838562

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
IL16 Antibody detects endogenous levels of total IL16.
RRID:
AB_2838562
引用格式: Affinity Biosciences Cat# DF6600, RRID:AB_2838562.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

HGNC:5980; HsT19289; IL-16; IL16; IL16_HUMAN; Interleukin 16 precursor; Interleukin-16; LCF; Lymphocyte chemoattractant factor; Neuronal interleukin 16; NIL16; prIL 16; PrIL16; Prointerleukin 16;

抗原和靶标

免疫原:

A synthesized peptide derived from human IL16, corresponding to a region within C-terminal amino acids.

基因/基因ID:
IL16(3603)
描述:
The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants.

文献引用

1). p.P476S mutation of RBPJL inhibits the efficacy of anti-PD-1 therapy in oesophageal squamous cell carcinoma by blunting T-cell responses. Clinical & Translational Immunology, 2020 (PubMed: 32994998) [IF=5.8]

Application: IHC    Species: Human    Sample:

Figure 5 CM of RBPJL knockdown cells inhibited the chemotaxis and proliferation of T cells and Th1/Th2 differentiation induced by CM from RBPJL‐overexpressing cells. (a) Representative images of RBPJL and IL‐16 staining in responsive oesophagus and lung lesions as well as liver lesions. Scale bars represent 200 μm. The number of positive cells from 3 to 5 fields was counted. The average positive ratio from each section was identified. (b) The chemotaxis of CD4+ and CD8+ T cells as analysed by transwell assay. (c) Effects of CMs from KYSE150 cells on the proliferation of T cells were assessed by loss of CFSE fluorescence. (d) Sorted naïve CD4+ T cells were stimulated by anti‐CD3/CD28 and human IL‐2, and subsequently incubated in the presence of CMs for 5 days, followed by staining with CD4, T‐bet and GATA3 antibodies for flow cytometry analysis. Percentages of CD4+ T‐bet+ and CD4+ GATA3+ T cells are shown. Data are presented as the mean ± standard deviation (n = 3), *P < 0.05, **P < 0.01, ***P < 0.001. The data represent three independent technical replicates.

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