TRAP220/MED1 Antibody - #DF6578

>>买2送1 已结束>>
>>赠JD卡 已结束>>
(2)   (4)  
产品: TRAP220/MED1 抗体
货号: DF6578
描述: Rabbit polyclonal antibody to TRAP220/MED1
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: Q15648
RRID: AB_2838540

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
TRAP220/MED1 Antibody detects endogenous levels of total TRAP220/MED1.
RRID:
AB_2838540
引用格式: Affinity Biosciences Cat# DF6578, RRID:AB_2838540.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Activator-recruited cofactor 205 kDa component; ARC205; CRSP1; CRSP200; DRIP205; DRIP230; MED1; MED1_HUMAN; Mediator complex subunit 1; Mediator of RNA polymerase II transcription subunit 1; p53 regulatory protein RB18A; PBP; Peroxisome proliferator-activated receptor-binding protein; PPAR binding protein; PPAR-binding protein; PPARBP; PPARGBP; RB18A; Thyroid hormone receptor-associated protein complex 220 kDa component; Thyroid receptor-interacting protein 2; TR-interacting protein 2; Trap220; TRIP-2; TRIP2; Vitamin D receptor-interacting protein complex component DRIP205;

抗原和靶标

免疫原:

A synthesized peptide derived from human TRAP220/MED1, corresponding to a region within the internal amino acids.

基因/基因ID:
MED1(5469)
描述:
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq, Jul 2008]

研究领域

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

文献引用

1). Chromosome instability region analysis and identification of the driver genes of the epithelial ovarian cancer cell lines A2780 and SKOV3. Journal of cellular and molecular medicine, 2023 (PubMed: 37525498) [IF=5.3]

Application: WB    Species: human    Sample:

FIGURE 7 Gene expression of 3 driver genes (MIEN1, GRB7 and MED1) in 3 cell lines (IOSE80, A2780 and SKOV3). (A) Western blot results. (B) Statistical diagram of western blot results. (C) Cell immunohistochemistry (IHC) results. (D) Statistical diagram of cell IHC results. All experiments were conducted in triplicate. Data are expressed as mean ± standard deviation. *p 

Application: IHC    Species: human    Sample:

FIGURE 7 Gene expression of 3 driver genes (MIEN1, GRB7 and MED1) in 3 cell lines (IOSE80, A2780 and SKOV3). (A) Western blot results. (B) Statistical diagram of western blot results. (C) Cell immunohistochemistry (IHC) results. (D) Statistical diagram of cell IHC results. All experiments were conducted in triplicate. Data are expressed as mean ± standard deviation. *p 
2). HOXA5 induces M2 macrophage polarization to attenuate carotid atherosclerosis by activating MED1. IUBMB LIFE, 2021 (PubMed: 34117711) [IF=3.7]

Application: WB    Species: Mouse    Sample: RAW264.7 cells

FIGURE 6 HOXA5 promoted M2 macrophage polarization by the co-regulation of MED1 in carotid arteries. (a) Immunofluorescent images for the colocalization of HOXA5 and CD163. (b, d) Relative mRNA levels of M1 (MARCO and MHCII) and M2 (PPARγ, Arg1, and MRC1) markers were detected by QPCR. (c, e) The levels of IL-6 and IL-10 were determined by ELISA. (f, g) Relative mRNA and protein levels of MED1 were examined using QPCR and Western blot. (h) Immunofluorescent images for the co-localization of MED1 and MAC- 3. & p < .05. n = 6. ELISA, enzyme-linked immunosorbent assay; HOXA5, homeobox A5; MED1, mediator subunit 1; QPCR, quantitative real-time reverse transcription PCR

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.