CD9 Antibody - #DF6565

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产品: CD9 抗体
货号: DF6565
描述: Rabbit polyclonal antibody to CD9
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: P21926
RRID: AB_2838527

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
CD9 Antibody detects endogenous levels of total CD9.
RRID:
AB_2838527
引用格式: Affinity Biosciences Cat# DF6565, RRID:AB_2838527.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Tetraspanin 29; 5H9; 5H9 antigen; Antigen defined by monoclonal antibody 602 29; Antigen defined by monoclonal antibody 60229; BA-2/p24 antigen; BA2; BTCC 1; BTCC1; CD9; CD9 antigen; CD9 antigen p24; CD9 molecule; CD9_HUMAN; Cell growth inhibiting gene 2 protein; Cell growth-inhibiting gene 2 protein; DRAP 27; DRAP27; GIG2; Growth inhibiting gene 2 protein; Leukocyte antigen MIC3; MIC3; Motility related protein; Motility-related protein; MRP 1; MRP-1; MRP1; p24; p24 antigen; Tetraspanin-29; Tspan 29; Tspan-29; TSPAN29;

抗原和靶标

免疫原:

A synthesized peptide derived from human CD9, corresponding to a region within the internal amino acids.

基因/基因ID:
CD9(928)
描述:
This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]

研究领域

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

文献引用

1). Intranasal delivery of engineered extracellular vesicles loaded with miR-206-3p antagomir ameliorates Alzheimer's disease phenotypes. Theranostics, 2024 (PubMed: 39659569) [IF=12.4]
2). Exosome from indoleamine 2,3-dioxygenase-overexpressing bone marrow mesenchymal stem cells accelerates repair process of ischemia/reperfusion-induced acute kidney injury by regulating macrophages polarization. Stem cell research & therapy, 2022 (PubMed: 35902956) [IF=7.5]
3). Extracellular vesicles delivering nuclear factor I/C for hard tissue engineering: Treatment of apical periodontitis and dentin regeneration. Journal of Tissue Engineering, 2022 (PubMed: 35321254) [IF=6.7]

Application: WB    Species: Human    Sample: SCAPs

Figure 2. Identification of EVs derived from LPS-stimulated DPCs (LPS-EVs) and establishment of in vitro model. (a) CCK8 assay detected cell viability of DPCs treated LPS (0.1, 1, 10, 100 µg/mL). (b) 1 µg/mL LPS increased EVs secretion of DPCs, compared to the other groups. (c) Schematic diagram shows the four types EV collected from EV-free culture medium of DPCs with or without LPS stimulation (0.1, 1, 10 µg/mL), which were used in subsequent assays. (d) Nanoparticle tracing assay (NTA) revealed the diameter of collected EVs was approximately 100 nm. (e) Scanning electron microscopy (SEM) of cup and saucer-shaped EVs. (f) Immunofluorescence staining confirmed that PKH26-labeled EVs (red) were endocytosed by SCAPs. DAPI (blue), and F-actin (green). (g) Western blot analysis of EVs surface markers (CD9, CD63, CD81 and TSG101). (h) CCK8 assay detected the effect of Nor-EV and LPS-EVs on cell viability of SCAPs. *p < 0.05. **p < 0.01. ***p < 0.001.#p < 0.0001.

Application: WB    Species: rat    Sample:

Figure 2. | Identification of EVs derived from LPS-stimulated DPCs (LPS-EVs) and establishment of in vitro model.(g) Western blot analysis of EVs surface markers (CD9, CD63, CD81 and TSG101).
4). Chemerin-Induced Down-Regulation of Placenta-Derived Exosomal miR-140-3p and miR-574-3p Promotes Umbilical Vein Endothelial Cells Proliferation, Migration, and Tube Formation in Gestational Diabetes Mellitus. Cells, 2022 (PubMed: 36359855) [IF=6.0]
5). Safety and biodistribution of exosomes derived from human induced pluripotent stem cells. Frontiers in bioengineering and biotechnology, 2022 (PubMed: 36091443) [IF=4.3]

Application: WB    Species: Mouse    Sample:

FIGURE 2. Characterization of exosomes derived from hiPSCs. (A) Cup-shaped structure of hiPSC-exosomes under a transmission electron microscope. (B) Particle size distribution map of hiPSC-exosomes generated by nanoparticle tracking analysis. (C) Specific protein markers (CD9 and TSG101) and negative protein marker (Calnexin) in hiPSC-exosomes detected by western blotting. (D) Isotype control IgG and specific markers (CD63, and CD81) in hiPSC-exosomes detected by a Flow NanoAnalyzer.
6). Extracellular vesicles of human glial cells exert neuroprotective effects via brain miRNA modulation in a rat model of traumatic brain injury. Scientific reports, 2023 (PubMed: 37989873) [IF=3.8]

Application: WB    Species: Human    Sample:

Figure 2 Characterization of extracellular vesicles released by human GPCs obtained from three independent donors. (A) Transmission electron microscopy images and size distribution charts for three isolates (co-plotted with the non-conditioned medium control data, blank). (B) Representative immunoblots showing the presence of tetraspanins CD81, CD9, and CD63 in three samples.
7). Bone marrow mesenchymal stem cells-derived exosomes ameliorate LPS-induced acute lung injury by miR-223-regulated alveolar macrophage M2 polarization. Journal of biochemical and molecular toxicology, 2024 (PubMed: 37899695) [IF=3.2]
8). Skin-inspired elastomer-hydrogel Janus fibrous membrane creates a superior pro-regenerative microenvironment toward complete skin regeneration. Biomaterials advances, 2025 (PubMed: 39951953)

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