(1)
(4)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6562, RRID:AB_2838524.
展开/折叠
HsMAD2; MAD 2; MAD2 like 1; MAD2 mitotic arrest deficient like 1; MAD2-like protein 1; Mad2l1; MD2L1_HUMAN; Mitotic arrest deficient 2-like protein 1; Mitotic spindle assembly checkpoint protein MAD2A; REV7;
抗原和靶标
- Q13257 MD2L1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALQLSREQGITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLVTTDLELIKYLNNVVEQLKDWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKDDSAPREKSQKAIQDEIRSVIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNSEEVRLRSFTTTIHKVNSMVAYKIPVND
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13257 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| Y77 | Phosphorylation | Uniprot | |
| C106 | S-Nitrosylation | Uniprot | |
| K108 | Acetylation | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| K111 | Ubiquitination | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| R129 | Methylation | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| S185 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| T187 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| K192 | Ubiquitination | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| Y199 | Phosphorylation | Uniprot | |
| K200 | Ubiquitination | Uniprot |
研究背景
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20. Phosphorylated by NEK2.
Nucleus. Chromosome>Centromere>Kinetochore. Cytoplasm. Cytoplasm>Cytoskeleton>Spindle pole.
Note: Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD.
Monomer and homodimer. Heterotetramer with MAD1L1. Formation of a heterotetrameric core complex containing two molecules each of MAD1L1 and of MAD2L1 promotes binding of another molecule of MAD2L1 to each MAD2L1, resulting in a heterohexamer. Interacts with CDC20, MAD2L1BP and with ADAM17/TACE. Dimeric MAD2L1 in the closed conformation interacts with CDC20. Monomeric MAD2L1 in the open conformation does not interact with CDC20. CDC20 competes with MAD1L1 for MAD2L1 binding. Interacts with TPR. Binds to UBD (via ubiquitin-like 1 domain) during mitosis. Interacts with isoform 1 and isoform 2 of NEK2. Interacts with HSF1; this interaction occurs in mitosis.
The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. The protein in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and of MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed form, and thereby promotes interaction with CDC20.
Belongs to the MAD2 family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
文献引用
Application: WB Species: Human Sample: breast cancer tissue
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