S100A8 Antibody - #DF6556

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产品: S100A8 抗体
货号: DF6556
描述: Rabbit polyclonal antibody to S100A8
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat
蛋白号: P05109
RRID: AB_2838518

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
S100A8 Antibody detects endogenous levels of total S100A8.
RRID:
AB_2838518
引用格式: Affinity Biosciences Cat# DF6556, RRID:AB_2838518.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

60B8Ag; AI323541; B8Ag; BEE11; CAGA; Calgranulin-A; Calprotectin L1L subunit; Calprotectin, included; CFAG; CGLA; Chemotactic cytokine CP-10; CP-10; Cystic fibrosis antigen; L1Ag; Leukocyte L1 complex light chain; MA387; MIF; Migration inhibitory factor-related protein 8; MRP-8; Myeloid-related protein 8; Neutrophil cytosolic 7 kDa protein; NIF; p8; Pro-inflammatory S100 cytokine; Protein S100-A8; S100 calcium binding protein A8 (calgranulin A); S100 calcium binding protein A8; S100 calcium-binding protein A8; S100A8; S100A8/S100A9 complex, included; S10A8_HUMAN; Urinary stone protein band A;

抗原和靶标

免疫原:

A synthesized peptide derived from human S100A8, corresponding to a region within C-terminal amino acids.

基因/基因ID:
S100A8(6279)
描述:
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq, Jul 2008]

研究领域

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

文献引用

1). S100A8 promotes epithelial‐mesenchymal transition and metastasis under TGF‐β/USF2 axis in colorectal cancer. Cancer Communications, 2021 (PubMed: 33389821) [IF=16.2]

Application: IHC    Species: human    Sample: CRC tissues

FIGURE 4| USF2 promotes migration and invasion in CRC cells and is associated with cancer progression and poor survival in CRC patients. A.USF2 overexpression enhanced cell migration and invasion in SW480 and HCT8 cells. B. USF2 knockdown inhibited cell migration and invasion in DLD1 and HT29 cells. C. Representative IHC images showed the coexpression of USF2 cytoplasmic staining (right panel) and S100A8 (left panel) in CRC tissues.

Application: WB    Species: human    Sample: HCT8 cells

FIGURE 2| S100A8 promoted EMT. A-B. S100A8 overexpression decreased E-cadherin, increased Vimentin and nuclear Snail, and changed the cells to a spindle-like morphology in SW480 (A) and HCT8 (B) cells.
2). Exosome-derived long non-coding RNA AC010789.1 modified by FTO and hnRNPA2B1 accelerates growth of hair follicle stem cells against androgen alopecia by activating S100A8/Wnt/β-catenin signalling. Clinical and translational medicine, 2025 (PubMed: 39748192) [IF=7.9]

Application: WB    Species: Mouse    Sample: HFSCs

FIGURE 5 S100A8 was identified as a downstream regulator of AC010789.1 to promote hair follicle stem cells (HFSCs) growth by activating Wnt/β-catenin signalling (A) RNA pull-down analysis of the interaction of S100A8 protein with the AC010789.1 mRNAs in HFSCs. (B) RIP analysis of the endogenous enrichment of AC010789.1 mRNAs in S100A8 protein in HFSCs. (C, D) Real-time quantitative polymerase chain reaction (qRT-PCR) and Western blot analysis of the expression levels of S100A8 after the transfection with AC010789.1 OE lentiviruses or si-AC010789.1 into HFSCs. (E, F) qRT-PCR and Western blot analysis of the expression levels of S100A8, K6HF and Lgr5 after the transfection of si-AC010789.1 into HFSCs. (G-I) CCK8, 5-ethynyl-2'-deoxyuridine assay (EdU) and Transwell analysis of the cell proliferation and migration viability after the co-transfection with AC010789.1 OE lentiviruses and si-S100A8 into HFSCs. (J) Western blot analysis of the expression levels of S100A8, Wnt10b, β-catenin and c-myc after the co-transfection with AC010789.1 OE lentiviruses and si-S100A8 into HFSCs. Data shown are the mean ± SEM of three experiments. **p < .01, ***p < .001 and ****p < .0001.
3). Transcriptomics Reveals Effect of Pulsatilla Decoction Butanol Extract in Alleviating Vulvovaginal Candidiasis by Inhibiting Neutrophil Chemotaxis and Activation via TLR4 Signaling. Pharmaceuticals (Basel, Switzerland), 2024 (PubMed: 38794163) [IF=4.6]

Application: WB    Species: Mouse    Sample:

Figure 13. BEPD downregulated the expression of chemokine-associated proteins (Western blots). Western blots were utilized to detect the expression of CXCL1, CXCL3, S100A8, and S100A9 proteins. Values are presented as mean ± SD. n = 3. ** p < 0.01, vs. control group; *** p < 0.01, vs. control group; # p < 0.05, vs. model group; ## p < 0.01, vs. model group; ### p < 0.001, vs. model group. BEPD, n-butanol extract of Pulsatilla decoction; BEPD-L, low-dose BEPD group (20 mL/kg); BEPD-M, medium-dose BEPD group (40 mL/kg); BEPD-H, high-dose BEPD group (80 mL/kg); Flu, fluconazole group.
4). Identification of S100A8/A9 involved in thromboangiitis obliterans development using tandem mass tags-labeled quantitative proteomics analysis. Cellular signalling, 2024 (PubMed: 38697446) [IF=4.4]
5). Proteomic and network pharmacology analyses reveal S100A8 as the anti-inflammatory target of Yunpi Jiedu Tongluo Qushi Granule in the treatment of rheumatoid arthritis. Journal of pharmaceutical and biomedical analysis, 2024 (PubMed: 39442465) [IF=3.1]
6). Unraveling the superiority of (−)-gallocatechin gallate to (−)-epigallocatechin-3-gallate in protection of diabetic nephropathy of db/db mice. Food Frontiers, 2024

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