Substance P Antibody - #DF6541

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产品: Substance P 抗体
货号: DF6541
描述: Rabbit polyclonal antibody to Substance P
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Sheep, Rabbit, Dog
蛋白号: P20366
RRID: AB_2838503

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
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说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Substance P Antibody detects endogenous levels of total Substance P.
RRID:
AB_2838503
引用格式: Affinity Biosciences Cat# DF6541, RRID:AB_2838503.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C-terminal-flanking peptide; neurokinin A; neurokinin alpha; NKA; NKNA; PPT; preprotachykinin; protachykinin; protachykinin-1; SubstanceP; TAC1; TAC2; Tachykinin 1; Tachykinin precursor 1; Tachykinin1; TKN1_HUMAN; SP;

抗原和靶标

免疫原:

A synthesized peptide derived from human Substance P, corresponding to a region within N-terminal amino acids.

基因/基因ID:
TAC1(6863)
描述:
This gene encodes four products of the tachykinin peptide hormone family, substance P and neurokinin A, as well as the related peptides, neuropeptide K and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

文献引用

1). Ginkgolide B effectively mitigates neuropathic pain by suppressing the activation of the NLRP3 inflammasome through the induction of mitophagy in rats. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38908197) [IF=6.9]
2). Estrogen receptor β/substance P signaling in spinal cord mediates antinociceptive effect in a mouse model of discogenic low back pain. Frontiers in cellular neuroscience, 2023 (PubMed: 36756381) [IF=5.3]

Application: IF/ICC    Species: Mouse    Sample: spinal cord

Figure 3. Spatial colocalization of substance P with ERβ in the spinal cord was investigated using dual-label confocal immunofluorescence examination. The specificities of the immunopositive staining of ERβ (green) and substance P (red) were demonstrated. Substance P colocalized with ERβ in the dorsal horn of the spinal cord, mainly in the laminae I and II, a connection site of pain transmission. (A) Coronal sections through the spinal cord of DLBP mice. Scale bar: 200 μm. (B) The magnified dorsal horn areas of the spinal cord of DLBP mice. Scale bar: 20 μm.
3). Substance P Mediates Estrogen Modulation Proinflammatory Cytokines Release in Intervertebral Disc. INFLAMMATION, 2021 (PubMed: 32965648) [IF=4.5]

Application: IHC    Species: mouse    Sample: NP cells

Fig. 1.| Effects of ERT on SP and its receptor NK1R in NP cells. After OVX, the staining intensity of NK1R (a–c) and SP (d–f) in NP cells significantly increased compared with the sham group. While OVX+E2 could reverse these changes. Bar represents 50 μm.
4). Electroacupuncture Stimulation Alleviates Inflammatory Pain in Male Rats by Suppressing Oxidative Stress. Physiological research, 2023 (PubMed: 38015764) [IF=1.9]

Application: WB    Species: Rat    Sample: C6 glioma cells

Fig. 5 ROS induced proinflammatory cytokine and CIP-related substance production in C6 glioma cells. C6 cells were treated with t-BOOH (50 μM) and PBN (100 μM) for 24 h. (A): ROS were assayed by a DCFHA probe (A1: CON; C2: t-BOOH; C3: PBN); (B–D): cytokines were measured by ELISA kits; (E): expression of p-p38, p-ERK, p-p65, SP, TRPV1 and TRPV4 was determined by western blotting. GAPDH was used as a loading control. t-BOOH: tert-butyl hydroperoxide; PBN: phenyl-N-tert-butylnitrone (ROS scavenger); data are represented as the mean ± standard deviation; n=4 per group; ## P

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