GRK2 Antibody - #DF6538

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产品: GRK2 抗体
货号: DF6538
描述: Rabbit polyclonal antibody to GRK2
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog, Chicken
蛋白号: P25098
RRID: AB_2838500

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
GRK2 Antibody detects endogenous levels of total GRK2.
RRID:
AB_2838500
引用格式: Affinity Biosciences Cat# DF6538, RRID:AB_2838500.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ADRBK1; Adrenergic beta receptor kinase 1; ARBK1_HUMAN; BARK; BARK1; Beta adrenergic receptor kinase 1; Beta ARK 1; Beta ARK1; Beta-adrenergic receptor kinase 1; Beta-ARK-1; FLJ16718; G protein coupled receptor kinase 2; G-protein coupled receptor kinase 2; GRK2;

抗原和靶标

免疫原:

A synthesized peptide derived from human GRK2, corresponding to a region within the internal amino acids.

基因/基因ID:
GRK2(156)
描述:
G-protein-coupled receptor kinase 2 (GRK2), also known as beta-adrenergic receptor kinase 1 (beta-ARK1), is a member of the GRK family, which phosphorylates the activated form of G-protein-coupled receptors (GPCRs) and initiates the desensitization process of GPCR (1). GRK2 kinase activity and cellular localization are tightly regulated by interactions with activated receptors, G-beta and G-gamma subunits, adaptor proteins, phospholipids, caveolin and calmodulin, as well as by phosphorylation (1). PKC phosphorylation enhances GRK2 activity by promoting its membrane localization and by abolishing the inhibitory association of calmodulin (2,3). PKA phosphorylates GRK2 at Ser685, which facilitates the association of GRK2 with a beta-adrenergic receptor (4). Erk inhibits GRK2 activity via phosphorylation at Ser670 (5). Src phosphorylates GRK2 at multiple tyrosine residues (Tyr13, 86 and 92), which activates GRK2 activity and promotes GRK2 degradation (6,7).

研究领域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Morphine addiction.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Sensory system > Olfactory transduction.

文献引用

1). Angiotensin II Type 2 Receptor Modulates Synovial Macrophage Polarization by Inhibiting GRK2 Membrane Translocation in a Rat Model of Collagen-Induced Arthritis. JOURNAL OF IMMUNOLOGY, 2020 (PubMed: 33148713) [IF=3.6]

Application: WB    Species: rat    Sample: Bone marrow–derived macrophages

FIGURE 9. AT2R activation inhibits GRK2 membrane translocation and promotes the coexpression with p-ERK1/2. BMDMs were pretreated with CGP42112 or PD123319 before the addition of LPS (1 mg/ml, 4 h). (A) The membrane protein levels of GRK2 were determined by flow cytometry (n = 5). (B) The total, membrane, and cytoplasm protein levels of GRK2 were determined by Western blot analyses (n = 4). (C) Confocal imaging of GRK2 translocation in BMDMs, Original magnification 3400. (D) LPS-stimulated BMDMs with CGP42112 or PD123319 were immunoprecipitated using an anti-GRK2 Ab and subjected to Western blotting to visualize ERK1/2 to evaluate its association with the GRK2 in cytoplasm. Coimmunoprecipitation (IP): anti-GRK2; immunoblot (IB): p-ERK1/2 and GRK2. (n = 3). **p , 0.01 versus control group; #p , 0.05, ##p , 0.01 versus LPS group; $p , 0.05, $$p , 0.01 versus LPS plus CGP group.
2). The ROS/GRK2/HIF-1α/NLRP3 Pathway Mediates Pyroptosis of Fibroblast-Like Synoviocytes and the Regulation of Monomer Derivatives of Paeoniflorin. Oxidative Medicine and Cellular Longevity, 2022 (PubMed: 35132350)

Application: WB    Species: Human    Sample:

Figure 5 G protein-coupled receptor kinase 2 (GRK2) promotes the expression of hypoxia-inducible factor (HIF)-1α. (a, b) shGRK2 was transfected into fibroblast-like synoviocytes (FLS) and exposed to hypoxia for 2 h. Immunoblot analysis and quantification of GRK2 and HIF-1α. (c, d) GRK2 overexpression plasmid was transfected into FLS. Immunoblot analysis and quantification of GRK2, HIF-1α, nucleotide-binding oligomerization segment-like receptor family 3 (NLRP3), and N-terminal domain of human gasdermin D (GSDMD-N). The values are shown as the mean ± standard deviation (n = 3). ∗p < 0.05, ∗∗p < 0.01 compared with the control group and #p < 0.05 compared with the hypoxia group or the NC group.

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