产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0831, RRID:AB_2833791.
展开/折叠
Amphiglycan; MGC22217; OTTHUMP00000031788; ryudocan amphiglycan; Ryudocan; Ryudocan core protein; SDC 4; Sdc4; SDC4_HUMAN; SYND 4; SYND4; syndecan 4; syndecan 4 (amphiglycan, ryudocan); syndecan proteoglycan 4; Syndecan-4; Syndecan4;
抗原和靶标
- P31431 SDC4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAPARLFALLLFFVGGVAESIRETEVIDPQDLLEGRYFSGALPDDEDVVGPGQESDDFELSGSGDLDDLEDSMIGPEVVHPLVPLDNHIPERAGSGSQVPTEPKKLEENEVIPKRISPVEESEDVSNKVSMSSTVQGSNIFERTEVLAALIVGGIVGILFAVFLILLLMYRMKKKDEGSYDLGKKPIYKKAPTNEFYA
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P31431 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S97 | O-Glycosylation | Uniprot | |
T101 | O-Glycosylation | Uniprot | |
K104 | Ubiquitination | Uniprot | |
S117 | O-Glycosylation | Uniprot | |
S126 | O-Glycosylation | Uniprot | |
T134 | Phosphorylation | Uniprot | |
K174 | Ubiquitination | Uniprot | |
S179 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
Y180 | Phosphorylation | P12931 (SRC) | Uniprot |
K190 | Ubiquitination | Uniprot | |
T193 | Phosphorylation | Uniprot | |
Y197 | Phosphorylation | Uniprot |
研究背景
Cell surface proteoglycan that bears heparan sulfate. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.
Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.
Membrane>Single-pass type I membrane protein. Secreted.
Note: Shedding of the ectodomain produces a soluble form.
Secreted.
Expressed in epithelial and fibroblastic cells.
Homodimer. Interacts (via its cytoplasmic domain) with GIPC (via its PDZ domain). Interacts (via its cytoplasmic domain) with NUDT16L1 (By similarity). Interacts with CDCP1 and SDCBP.
Belongs to the syndecan proteoglycan family.
研究领域
· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
文献引用
Application: IHC Species: Rat Sample:
Application: WB Species: Rat Sample:
Application: IHC Species: Rat Sample:
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