Syndecan 4 Antibody - #AF0831

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产品: Syndecan 4 抗体
货号: AF0831
描述: Rabbit polyclonal antibody to Syndecan 4
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
蛋白号: P31431
RRID: AB_2833791

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Syndecan 4 Antibody detects endogenous levels of total Syndecan 4.
RRID:
AB_2833791
引用格式: Affinity Biosciences Cat# AF0831, RRID:AB_2833791.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Amphiglycan; MGC22217; OTTHUMP00000031788; ryudocan amphiglycan; Ryudocan; Ryudocan core protein; SDC 4; Sdc4; SDC4_HUMAN; SYND 4; SYND4; syndecan 4; syndecan 4 (amphiglycan, ryudocan); syndecan proteoglycan 4; Syndecan-4; Syndecan4;

抗原和靶标

免疫原:

A synthesized peptide derived from human Syndecan 4, corresponding to a region within C-terminal amino acids.

基因/基因ID:
SDC4(6385)
描述:
The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. The encoded protein is found as a homodimer and is a member of the syndecan proteoglycan family. This gene is found on chromosome 20, while a pseudogene has been found on chromosome 22. SwissProt Information

研究领域

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

文献引用

1). Necroptotic TNFα‐syndecan 4‐TNFα vicious cycle as a therapeutic target for preventing temporomandibular joint osteoarthritis. Journal of Bone and Mineral Research, 2022 (PubMed: 35278225) [IF=5.1]

Application: IHC    Species: Rat    Sample:

Fig. 5 SDC4 as specific necroptotic DAMPs contributing to cartilage degeneration and synovial inflammation. (A) Cell morphology was explored by interference light microscopy after treatment with different agents. (B) TNFα, ADAMTS5, and MMP13 in the culture supernatants in different groups were measured by ELISA (n = 4). (C) Western blot analysis of TNFα, ADAMTS5, MMP13, ACAN, RIPK3, and pMLKL in the different groups. (D) Quantitative data of C (n = 3). (E) IHC staining of SDC4, RIPK3, and pMLKL in different groups. (F) Quantitative data of E (n = 6). (G) H&E staining and TNFα-IHC staining of cartilage and synovium in different groups. (H) Quantitative data of G. (n = 6). Scale bars = 100 μm. Unpaired t test was used to compare the data between two groups.

Application: WB    Species: Rat    Sample:

Fig. 5 SDC4 as specific necroptotic DAMPs contributing to cartilage degeneration and synovial inflammation. (A) Cell morphology was explored by interference light microscopy after treatment with different agents. (B) TNFα, ADAMTS5, and MMP13 in the culture supernatants in different groups were measured by ELISA (n = 4). (C) Western blot analysis of TNFα, ADAMTS5, MMP13, ACAN, RIPK3, and pMLKL in the different groups. (D) Quantitative data of C (n = 3). (E) IHC staining of SDC4, RIPK3, and pMLKL in different groups. (F) Quantitative data of E (n = 6). (G) H&E staining and TNFα-IHC staining of cartilage and synovium in different groups. (H) Quantitative data of G. (n = 6). Scale bars = 100 μm. Unpaired t test was used to compare the data between two groups.
2). Necroptotic TNFα-Syndecan 4-TNFα Vicious Cycle as a Therapeutic Target for Preventing Temporomandibular Joint Osteoarthritis. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 2022 (PubMed: 35278225) [IF=5.1]

Application: IHC    Species: Rat    Sample:

Fig. 5. SDC4 as specific necroptotic DAMPs contributing to cartilage degeneration and synovial inflammation. (A) Cell morphology was explored by inter-ference light microscopy after treatment with different agents. (B) TNFα, ADAMTS5, and MMP13 in the culture supernatants in different groups were mea-sured by ELISA (n = 4). (C) Western blot analysis of TNFα, ADAMTS5, MMP13, ACAN, RIPK3, and pMLKL in the different groups. (D) Quantitative data ofC (n = 3). (E) IHC staining of SDC4, RIPK3, and pMLKL in different groups. (F) Quantitative data of E (n = 6). (G) H&E staining and TNFα-IHC staining of car-tilage and synovium in different groups. (H) Quantitative data of G. (n = 6). Scale bars = 100 μm. Unpaired t test was used to compare the data betweentwo groups.

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