HMGCR Antibody - #DF6518

Fig. 4. β-PAE inhibits the expression of hepatic lipid synthesis-related proteins and genes in HFD-fed rats. (A–F) Western blot analysis on the expression of proteins referred to hepatic lipid synthesis including SREBP-1c, ACC1, p-ACC1, FASN, SCD1 and HMG-CR; (G–H) The mRNA expression of SREBP-1c and HMG-CR. Data are presented as the mean ± SD (n = 6~8). ##p < 0.01 vs. NC group; *p < 0.05, **p < 0.01 vs. Model group.

Fig. 1 The significant decrease of hippocampal cholesterol level in 5xFAD mice. A Quantification of cholesterol level in hippocampus of WT and 5xFAD mice (3 month) by LC–MS/MS analysis. n = 3–4 sample/group, every sample was included 2–3 mice randomly. B–D The fold change expression of mRNA of DHCR24, HMGCR and SREBP2 in hippocampus of WT and 5xFAD mice (3 month) by RT-PCR. E The immunoblotting bands of DHCR24, HMGCR, SREBP2 in hippocampus of 3-month-old WT mice and 5xFAD mice. F Analysis of western blot with mean gray value which all were quantification on the ratio of target proteins against GAPDH. G The fluorescence images of DHCR24 (red) in hippocampus of 3-month-old WT and 5xFAD mice (scale bar, 20 μm). H Mean fluorescence intensity of DHCR24. n = 3 mice per group in [B-H]. Data expressed as mean ± SEM, statistical analysis was performed using unpaired two-tailed Student’s t-test. *P 

Fig. 2. |NPY promotes HMGCR protein expression both in vivo and in vitro.(A) Hepatic portal vein injection of NPY (100 μg/kg) significantly increased HMGCR expression after 1 h in rats.

FIG 1 (A) Cholesterol synthesis pathway. (B) Heat map of the key genes (transcriptomic data) and corresponding proteins (proteomic data) involved in lipid metabolism in bovine viral diarrhea virus (BVDV)-infected bovine cells. (C, D) Identification of differentially expressed genes by quantitative reverse transcription-PCR (qRT-PCR) (C) and corresponding proteins by Western blotting (D). HMGCS1, 3- hydroxy-3-methylglutaryl-coenzyme A synthase 1; HMGCR, 3-hydroxy-3-methylglutaryl-coenzyme A reductase; MVK, mevalonate kinase; PMVK, phosphomevalonate kinase; MVD, diphosphomevalonate decarboxylase; FDPS, farnesyl diphosphate synthase; FDFT1, farnesyl diphosphate farnesyltransferase 1; SQLE, squalene epoxidase; GGPS, geranylgeranyl pyrophosphate synthase; LSS, lanosterol synthase; D8D7I, 3β-hydroxysterol-Δ8,7-isomerase; C5SD, 3β-hydroxysterol-C5 desaturase; DHCR7, 7-dehydrocholesterol reductase; DHCR24, 3β-hydroxysteroid-Δ24 reductase; SCD1, stearoyl-CoA desaturase; FASN, fatty acid synthase; ACACA, acetyl coenzyme A carboxylase alpha.