GSTA1 Antibody - #DF6514

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产品: GSTA1 抗体
货号: DF6514
描述: Rabbit polyclonal antibody to GSTA1
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Chicken, Xenopus
分子量: 26kDa; 26kD(Calculated).
蛋白号: P08263
RRID: AB_2838476

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(91%), Bovine(91%), Horse(92%), Sheep(91%), Chicken(82%), Xenopus(91%)
克隆:
Polyclonal
特异性:
GSTA1 Antibody detects endogenous levels of total GSTA1.
RRID:
AB_2838476
引用格式: Affinity Biosciences Cat# DF6514, RRID:AB_2838476.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Glutathione S alkyltransferase A1; Glutathione S aryltransferase A1; Glutathione S transferase 2; Glutathione S transferase A1; Glutathione S transferase alpha 1; Glutathione S transferase Ha subunit 1; Glutathione S-transferase A1; GST class-alpha member 1; GST epsilon; GST HA subunit 1; GST, class alpha, 1; GST-epsilon; GST2; GSTA1 1; GSTA1; GSTA1-1; GSTA1_HUMAN; GTH1; HA subunit 1; MGC131939; OTTHUMP00000016611; S (hydroxyalkyl)glutathione lyase A1;

抗原和靶标

免疫原:
Uniprot:

P08263(GSTA1_HUMAN) >>Visit HPA database.

基因/基因ID:
GSTA1(2938)
表达:
P08263 GSTA1_HUMAN:

Liver.

描述:
Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver. In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity thereby protecting the cells from reactive oxygen species and the products of peroxidation. [provided by RefSeq, Jul 2008]
序列:
MAEKPKLHYFNARGRMESTRWLLAAAGVEFEEKFIKSAEDLDKLRNDGYLMFQQVPMVEIDGMKLVQTRAILNYIASKYNLYGKDIKERALIDMYIEGIADLGEMILLLPVCPPEEKDAKLALIKEKIKNRYFPAFEKVLKSHGQDYLVGNKLSRADIHLVELLYYVEELDSSLISSFPLLKALKTRISNLPTVKKFLQPGSPRKPPMDEKSLEEARKIFRF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
92
Pig
91
Bovine
91
Sheep
91
Xenopus
91
Chicken
82
Dog
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P08263 作为底物

Site PTM Type Enzyme
Y9 Phosphorylation
S37 Phosphorylation
Y49 Phosphorylation
Y74 Phosphorylation
S77 Phosphorylation
Y79 Phosphorylation
Y82 Phosphorylation
K84 Acetylation
K87 Acetylation
K125 Ubiquitination
Y132 Phosphorylation
S142 Phosphorylation
Y147 Phosphorylation
K152 Acetylation
S154 Phosphorylation
S176 Phosphorylation
S177 Phosphorylation
S189 Phosphorylation
T193 Phosphorylation
K195 Acetylation
K196 Acetylation
S202 Phosphorylation
S212 Phosphorylation

研究背景

功能:

Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione and may therefore play an important role in hormone biosynthesis. Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Liver.

亚基结构:

Homodimer or heterodimer of GSTA1 and GSTA2.

蛋白家族:

The C-terminal domain may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity.

Belongs to the GST superfamily. Alpha family.

研究领域

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

文献引用

1). JNK signaling pathway mediates acetaminophen-induced hepatotoxicity accompanied by changes of glutathione S-transferase A1 content and expression. Frontiers in Pharmacology (PubMed: 31620005) [IF=5.6]

Application: WB    Species: mouse    Sample: liver

FIGURE 7 | Changes of GSTA1 caused by different dosages of APAP in mice with or without SP600125. (C) Western blot analyses of total tissue lysate for GSTA1 and β-actin (loading control), n = 3.
2). Acetaminophen-induced hepatocyte injury: C2-ceramide and oltipraz intervention, hepatocyte nuclear factor 1 and glutathione S-transferase A1 changes. JOURNAL OF APPLIED TOXICOLOGY (PubMed: 31385618) [IF=3.3]

Application: WB    Species:    Sample: hepatocytes

FIGURE 6| A, Original blots for HNF‐1, GSTA1 and β‐actin. B, HNF‐1 protein level. C, GSTA1 protein level. Changes in HNF‐1 and GSTA1 protein levels in hepatocytes after 15 mmol/L APAP exposure for 10 h with C2 (6 μmol/L) or OL (8 μmol/L). Values are expressed as the mean ± SD for each group (n = 4). *P < .05, **P < .01 represented all groups compared with the control group; #P < .05, ##P < .01 represented all groups compared with the APAP model group. APAP, acetaminophen; C2, C2‐ceramide; GSTA1, glutathione S‐transferase A1; HNF‐1, hepatocyte nuclear factor 1; OL, oltipraz
3). Acetaminophen-induced reduction in glutathione-S-transferase A1 in hepatocytes: A role for hepatic nuclear factor 1α and its response element. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (PubMed: 31230750) [IF=3.1]

Application: WB    Species: human    Sample: HepG2 cells

Fig. 3. |Effects of C2-ceramide or oltipraz on the nuclear HNF-1a levels and the GSTA1 protein expression in HepG2 cells with injury induced by APAP (n ¼ 4). *P < 0.05, **P < 0.01 compared with control group; #P < 0.05, ##P < 0.01 compared with model group.
4). Prevention of acetaminophen-induced hepatocyte injury: JNK inhibition and GSTA1 involvement. Molecular & Cellular Toxicology [IF=1.7]

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