产品: TFPI 抗体
货号: DF6511
描述: Rabbit polyclonal antibody to TFPI
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Chicken
分子量: 35kDa; 35kD(Calculated).
蛋白号: P10646
RRID: AB_2838473

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(86%), Bovine(86%), Horse(86%), Rabbit(100%), Chicken(83%)
克隆:
Polyclonal
特异性:
TFPI Antibody detects endogenous levels of total TFPI.
RRID:
AB_2838473
引用格式: Affinity Biosciences Cat# DF6511, RRID:AB_2838473.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Anti convertin; EPI; Extrinsic pathway inhibitor; LACI; Lipoprotein associated coagulation inhibitor; Lipoprotein-associated coagulation inhibitor; TFI; TFPI 1; TFPI; TFPI1; TFPI1_HUMAN; Tissue factor pathway inhibitor (lipoprotein associated coagulation inhibitor); Tissue factor pathway inhibitor;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P10646 TFPI1_HUMAN:

Mostly in endothelial cells.

描述:
This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. [provided by RefSeq, Jul 2008]
序列:
MIYTMKKVHALWASVCLLLNLAPAPLNADSEEDEEHTIITDTELPPLKLMHSFCAFKADDGPCKAIMKRFFFNIFTRQCEEFIYGGCEGNQNRFESLEECKKMCTRDNANRIIKTTLQQEKPDFCFLEEDPGICRGYITRYFYNNQTKQCERFKYGGCLGNMNNFETLEECKNICEDGPNGFQVDNYGTQLNAVNNSLTPQSTKVPSLFEFHGPSWCLTPADRGLCRANENRFYYNSVIGKCRPFKYSGCGGNENNFTSKQECLRACKKGFIQRISKGGLIKTKRKRKKQRVKIAYEEIFVKNM

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Rabbit
100
Pig
86
Horse
86
Bovine
86
Chicken
83
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P10646 作为底物

Site PTM Type Enzyme
S30 Phosphorylation P68400 (CSNK2A1)
T42 O-Glycosylation
T115 O-Glycosylation
T116 O-Glycosylation
K121 Ubiquitination
N145 N-Glycosylation
N195 N-Glycosylation
S202 O-Glycosylation
S202 Phosphorylation P28482 (MAPK1)
T203 O-Glycosylation
T219 Phosphorylation
K260 Ubiquitination
K268 Acetylation
K269 Acetylation
K269 Sumoylation
K293 Sumoylation

研究背景

功能:

Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.

翻译修饰:

O-glycosylated.

细胞定位:

Secreted.

Microsome membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Mostly in endothelial cells.

蛋白家族:

This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa.

研究领域

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

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