产品: Claudin 2 抗体
货号: AF0128
描述: Rabbit polyclonal antibody to Claudin 2
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 25kDa; 25kD(Calculated).
蛋白号: P57739
RRID: AB_2833312

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
Claudin 2 Antibody detects endogenous levels of total Claudin 2.
RRID:
AB_2833312
引用格式: Affinity Biosciences Cat# AF0128, RRID:AB_2833312.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, sodium azide and glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Claudin-2; Claudin2; CLD2_HUMAN; CLDN 2; CLDN2; OTTHUMP00000023793; SP 82; SP82;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
claudin 2 Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Belongs to the claudin family. Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN3, but not CLDN1, homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3
序列:
MASLGLQLVGYILGLLGLLGTLVAMLLPSWKTSSYVGASIVTAVGFSKGLWMECATHSTGITQCDIYSTLLGLPADIQAAQAMMVTSSAISSLACIISVVGMRCTVFCQESRAKDRVAVAGGVFFILGGLLGFIPVAWNLHGILRDFYSPLVPDSMKFEIGEALYLGIISSLFSLIAGIILCFSCSSQRNRSNYYDAYQAQPLATRSSPRPGQPPKVKSEFNSYSLTGYV

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
78
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P57739 作为底物

Site PTM Type Enzyme
S192 Phosphorylation
Y194 Phosphorylation
Y195 Phosphorylation
Y198 Phosphorylation
S208 Phosphorylation
S219 Phosphorylation
S223 Phosphorylation
Y224 Phosphorylation
S225 Phosphorylation

研究背景

功能:

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

翻译修饰:

The disulfide bond is necessary for pore formation, but is not required for correct protein trafficking.

细胞定位:

Cell junction>Tight junction. Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN3, but not CLDN1, homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 (By similarity).

蛋白家族:

Belongs to the claudin family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

文献引用

1). Commensal cow Roseburia reduces gut-dysbiosis-induced mastitis through inhibiting bacterial translocation by producing butyrate in mice. Cell Reports, 2022 (PubMed: 36417859) [IF=8.8]

Application: WB    Species: Mouse    Sample:

Figure 5Commensal Roseburia ameliorates gut-dysbiosis-induced mastitis in mice

2). Mesenchymal Stem Cells Overexpressing ACE2 Favorably Ameliorate LPS-Induced Inflammatory Injury in Mammary Epithelial Cells. Frontiers in Immunology, 2022 (PubMed: 35095873) [IF=7.3]

Application: WB    Species: Mice    Sample: EpH4-Ev cells

Figure 8 MSC-ACE2 upregulates blood-milk barrier-related protein expression to suppress LPS-induced inflammation in Eph4-EV cells. (A) Detection of relative protein expression levels of ZO-1, Claudin-1, Claudin-2 by Western blot; (B) Statistics of the ZO-1, Claudin-1, Claudin-2 Western blot results. Experiments were repeated three times and data were presented as the mean ± SEM (n = 4). * P < 0.05 vs. EpH4-Ev; # P < 0.05 vs. LPS; $ P < 0.05 vs. MSC; & P < 0.05 vs. MSC-GFP.

3). Effects of Dietary Colostrum Basic Protein on Bone Growth and Calcium Absorption in Mice. Nutrients, 2024 (PubMed: 38474792) [IF=5.9]

Application: WB    Species: Mouse    Sample:

Figure 5 Effect of CBP on calcium absorption and related proteins in mice. (a) Apparent absorption rate of calcium. (b–f) TRPV6, PMCA1, CaBP-9k, CLD2, and CLD12 in jejunum, (g,h) CLD2 and CLD12 in ileum levels in the five groups. Data are presented as mean ± SEM (n = 3). Statistical significance between groups is represented by different lowercase letters (p < 0.05).

4). Matrine protects against DSS-induced murine colitis by improving gut barrier integrity, inhibiting the PPAR-α signaling pathway, and modulating gut microbiota. International Immunopharmacology, 2021 (PubMed: 34474274) [IF=5.6]

Application: WB    Species: Mice    Sample: colon tissues

Fig. 4. Matrine improved gut barrier integrity in DSS-induced UC mice. (A) AB/ PAS and immunofluorescence imaging detected the mucin-producing cells and the expression of mucin-2, respectively. (B) Analysis of the expression levels of the mucin-producing cells and mucin-2. (C) The expressions of occludin, claudin-1,2, and ZO- 1 protein were verified by western blot. (D) Analysis of the expression levels of occludin, claudin-1,2, and ZO-1. Data are expressed as means ± SEM; n = 5 for each group. * P < 0.05, ** P < 0.01, *** P < 0.001, and ****P < 0.0001.

Application: WB    Species: mouse    Sample: colon

Fig. 4. |Matrine improved gut barrier integrity in DSS-induced UC mice.(C) The expressions of occludin, claudin-1,2, and ZO1 protein were verified by western blot.

5). The Combined Effect of Vitamin C and Vitamin D3 on the Intestinal Epithelial Barrier by Regulating Notch Signaling Pathway. Nutrition & Metabolism, 2021 (PubMed: 33964955) [IF=4.5]

Application: WB    Species: Human    Sample: SW480 cells

Fig. 4 Expression of tight junction-associated proteins and genes and Notch signaling pathway-related proteins and genes in SW480 cells. 1: control group; 2: LPS group; 3: VD3 group; 4: 0.1VC+ VD3 group; 5: 1VC+ VD3 group; 6: 5VC+ VD3 group; 7: 10VC+ VD3 group. a Western blotting results of claudin-2 in SW480 cells, b western blotting results of ZO-1 in SW480 cells, c western blotting results of Notch intracellular domain (NICD) in SW480 cells, d western blotting results of Hes-1 in SW480 cells, e RT-PCR of tight junction protein-related genes and Notch signaling pathway-related genes. In the fgure, a–f indicate signifcant diferences compared with the control group, LPS group, VD3 group, 0.1VC+ VD3 group, 1VC+ VD3 group, and 5VC+ VD3 group, respectively

Application: WB    Species: Human    Sample: SW480 cells

Fig. 4 Expression of tight junction-associated proteins and genes and Notch signaling pathway-related proteins and genes in SW480 cells. 1: control group; 2: LPS group; 3: VD3 group; 4: 0.1VC+ VD3 group; 5: 1VC+ VD3 group; 6: 5VC+ VD3 group; 7: 10VC+ VD3 group. a Western blotting results of claudin-2 in SW480 cells, b western blotting results of ZO-1 in SW480 cells, c western blotting results of Notch intracellular domain (NICD) in SW480 cells, d western blotting results of Hes-1 in SW480 cells, e RT-PCR of tight junction protein-related genes and Notch signaling pathway-related genes. In the fgure, a–f indicate signifcant diferences compared with the control group, LPS group, VD3 group, 0.1VC+ VD3 group, 1VC+ VD3 group, and 5VC+ VD3 group, respectively

6). Lacticaseibacillus casei IB1 Alleviates DSS-Induced Inflammatory Bowel Disease by Regulating the Microbiota and Restoring the Intestinal Epithelial Barrier. Microorganisms, 2024 (PubMed: 39065147) [IF=4.5]

7). Cr (VI) promotes tight joint and oxidative damage by activating the Nrf2/ROS/Notch1 axis. Environmental Toxicology and Pharmacology, 2021 (PubMed: 33757840) [IF=4.3]

Application: IHC    Species: mice    Sample: intestine

Fig. 1. Cr(VI)-induced tight joint and oxidative damage in the small intestine of mice. Cr(VI)- induced tight joint damage in the small intestine of mice (A–F). After Cr(VI) treatment, Zo- 2 and occludin protein levels were examined via Western blot analysis (A), and the relative ratios of Zo-2 and occludin were quantified in a histogram (B and C). Histopathological changes in the small intestine (D). IHC staining of Zo-1 and claudin-2 (E and F). The first lines of D, E, and F were the respective control groups (I and II), and the second line corresponded to 40 mg/kg Cr groups (III and IV). Higher magnification images of the outlined area are shown on the right (II and IV). Cr(VI)-induced oxidative damage in the small intestine of mice (G–I). Small intestinal homogenate samples were collected to examine the levels of SOD (G), MDA (H), and GSH-Px (I). Values in the same column with different superscripts (a, b, c, d) significantly differed and represented as mean ± SD. Differences with p < 0.05 were presented. All experimental data were obtained from three independent experiments.

8). Protective mechanisms of Agrimonia pilosa Ledeb in dextran sodium sulfate-induced colitis as determined by a network pharmacology approach. ACTA BIOCHIMICA ET BIOPHYSICA SINICA, 2021 (PubMed: 34523667) [IF=3.7]

9). Changes in intestinal barrier protein expression and intestinal flora in a rat model of visceral hypersensitivity. NEUROGASTROENTEROLOGY AND MOTILITY, 2022 (PubMed: 34821442) [IF=3.5]

10). P38α deficiency in macrophages ameliorates murine experimental colitis by regulating inflammation and immune process. PATHOLOGY RESEARCH AND PRACTICE, 2022 (PubMed: 35430508) [IF=2.8]

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