BTK Antibody - #DF6472

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产品: BTK 抗体
货号: DF6472
描述: Rabbit polyclonal antibody to BTK
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: Q06187
RRID: AB_2838434

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
BTK Antibody detects endogenous levels of total BTK.
RRID:
AB_2838434
引用格式: Affinity Biosciences Cat# DF6472, RRID:AB_2838434.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Agammaglobulinaemia tyrosine kinase; AGMX 1; AGMX1; AT; ATK; B cell progenitor kinase; B-cell progenitor kinase; BPK; Bruton agammaglobulinemia tyrosine kinase; Bruton tyrosine kinase; Bruton’s Tyrosine Kinase; Btk; BTK_HUMAN; dominant-negative kinase-deficient Brutons tyrosine kinase; IMD 1; IMD1; MGC126261; MGC126262; OTTHUMP00000063593; PSCTK 1; PSCTK1; truncated Bruton agammaglobulinemia tyrosine kinase; Tyrosine protein kinase BTK; Tyrosine-protein kinase BTK; tyrosine-protein kinase BTK isoform (lacking exon 14; XLA;

抗原和靶标

免疫原:

A synthesized peptide derived from human BTK, corresponding to a region within the internal amino acids.

基因/基因ID:
BTK(695)
描述:
Bruton's tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases. Like other Btk family members, it contains a pleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. Btk plays an important role in B cell development (1,2). Activation of B cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate (3-5). The membrane-localized Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinases, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation (6,7). The activation of Btk is negatively regulated by PKCβ through phosphorylation of Btk at Ser180, which results in reduced membrane recruitment, transphosphorylation, and subsequent activation (8). The PKC inhibitory signal is likely to be a key determinant of the B cell receptor signaling threshold to maintain optimal Btk activity (8).

研究领域

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Human Diseases > Immune diseases > Primary immunodeficiency.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

文献引用

1). Zanubrutinib Ameliorates Cardiac Fibrosis and Inflammation Induced by Chronic Sympathetic Activation. Molecules (Basel, Switzerland), 2023 (PubMed: 37630287) [IF=4.6]
2). REC8 enhances stemness and promotes metastasis of colorectal cancer through BTK/Akt/β-catenin signaling pathway. Translational Oncology, 2022 (PubMed: 34890967) [IF=4.5]

Application: IHC    Species: Mice    Sample: CRC cells

Fig. 6 Overexpression of REC8 promotes CRC liver metastasis and elevates BTK/β-catenin signaling in vivo. A total of 2 ×   106 DLD-1 LV-Ctrl and DLD-1 LV-REC8 cells were injected into the spleens of ten male nude mice respectively. Twelve weeks later, the mice were sacrificed. (A) Representative images of mice livers and number of metastatic nodules after intrasplenic injection of CRC cells (N = 5 each group). Red narrow: metastatic nodules. (B) Representative images of HE staining of metastatic nodules in the livers. Scale bars in white, 200 μm. Scale bars in black, 50 μm. (C) Representative images of IHC staining for REC8, BTK, and β-catenin of metastatic nodules in the livers. Scale bars, 50 μm. *P < 0.05.

Application: WB    Species: Mice    Sample: CRC cells

Fig. 5 Inhibition of BTK suppresses migration, invasion, and stemness of CRC cells by BTK/Akt/β-catenin pathway. (A) Gene set enrichment analysis of up-regulated and down-regulated mRNAs upon REC8 overexpression. (B) Wound-healing assay of influences of BTK inhibitor ibrutinib (1 μM/L) with REC8 overexpression on cell migration at 48 h. Scale bars, 500 μm. (C) Transwell assay of effects of BTK inhibitor ibrutinib (1 μM/L) with REC8 overexpression on cell invasion at 24 h. Scale bars, 200 μm. (D) Western blot analysis of effects of BTK inhibitor ibrutinib with REC8 overexpression on stem cell characters. (E) Western blot analysis of effects of BTK inhibitor ibrutinib with REC8 overexpression on p-BTK, BTK, p-Akt, Akt, and β-catenin. Data represented the mean ± SEM from three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001, IBRU is short for ibrutinib.
3). Inhibition of Bruton's Tyrosine Kinase Protects Against Burn Sepsis-Induced Intestinal Injury. Frontiers in Medicine, 2022 (PubMed: 35280898) [IF=3.9]

Application: WB    Species: Mice    Sample: intestinal tissue

Figure 1 Expression levels of total BTK and p-BTK in intestinal tissue. (A) A representative western blot image of total BTK protein and p-BTK in each group. (B) The expression level of p-BTK (p-BTK /BTK) in each group at different time points of postburn. *P < 0.05, vs. the sham group and burn group; in the burn + sepsis group, #P < 0.05, vs. 0h; &P < 0.05, vs. 8h; ΔP < 0.05, vs. 12h; ▴P < 0.05, ▴▴P < 0.01, vs. the burn + sepsis group at corresponding time points except 0 h of postburn.
4). Zanubrutinib ameliorates experimental idiopathic inflammatory myopathy-associated interstitial lung disease by BTK/NF-κB signaling pathway. Research Square, 2024

Application: WB    Species: Mouse    Sample:

Figure 5. Zanubrutinib inhibits phosphorylation of BTK and NF-κB in the IIM-ILD model. (a) Expression of changes in fibrosis-related indexes, including α-SMA, FN, COL1, and BTK, as well as NF-κB and their phosphorylation-related proteins, were analyzed by western blotting; (b、c、d、e、f) Statistical values are expressed as mean ±SD. *:p＜0.05; **:p＜0.01.

Application: WB    Species: Mouse    Sample:

Figure 5. Zanubrutinib inhibits phosphorylation of BTK and NF-κB in the IIM-ILD model. (a) Expression of changes in fibrosis-related indexes, including α-SMA, FN, COL1, and BTK, as well as NF-κB and their phosphorylation-related proteins, were analyzed by western blotting; (b、c、d、e、f) Statistical values are expressed as mean ±SD. *:p＜0.05; **:p＜0.01.

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