Tryptophan Hydroxylase Antibody - #DF6465

Figure 1.5-HT biosynthesis and secretion are upregulated in colorectal cancer cells. A, Kaplan–Meier overall survival curve of patients with colorectal cancer from TCGA based on expression of TPH1 in tumor tissues. Patients were divided into a TPH1-high expression group (n = 76) and a TPH1-low expression group (n = 304). B, mRNA expression of TPH1 in normal and tumor tissues of patients with colorectal cancer was detected by real-time PCR. C and D, Quantitative analysis of TPH1 protein expression and western blots in normal (N) and tumor (T) tissues of patients with colorectal cancer from B. B and C, Paired Student t test, n = 21. E, Representative immunofluorescence photos for TPH1 in normal and tumor tissues of colorectal cancer patients P7#, P9#, and P14#. F, mRNA expression of TPH1 in normal colorectal epithelial cell lines (FHS and FHC) and 153 colorectal cancer (CRC) cell lines from GEO database (GSE59857); unpaired t test. G, mRNA expression of TPH1 in the human normal colorectal epithelial cell line NCM460 and four human colorectal cancer cell lines (SW480, SW620, HCT116, and HT29), as well as THP-1. H, mRNA expression of Tph1 in mouse primary CECs and mouse colorectal cancer cell line CT26, as well as iBMDMs. I, Protein expression of TPH1 in NCM460, SW480, SW620, HCT116, HT29, and THP-1 cells, as well as CECs, CT26 cells, and iBMDMs. J and K, 5-HT concentrations in supernatants of NCM460, SW480, SW620, HCT116, HT29, and THP-1 cells, as well as CECs, CT26 cells, and iBMDMs detected by ELISA. G–K, Data represent mean ± SD for at least three independent experiments; one-way ANOVA; N.D., not detected. **, P < 0.01.

Figure 1.5-HT biosynthesis and secretion are upregulated in colorectal cancer cells. A, Kaplan–Meier overall survival curve of patients with colorectal cancer from TCGA based on expression of TPH1 in tumor tissues. Patients were divided into a TPH1-high expression group (n = 76) and a TPH1-low expression group (n = 304). B, mRNA expression of TPH1 in normal and tumor tissues of patients with colorectal cancer was detected by real-time PCR. C and D, Quantitative analysis of TPH1 protein expression and western blots in normal (N) and tumor (T) tissues of patients with colorectal cancer from B. B and C, Paired Student t test, n = 21. E, Representative immunofluorescence photos for TPH1 in normal and tumor tissues of colorectal cancer patients P7#, P9#, and P14#. F, mRNA expression of TPH1 in normal colorectal epithelial cell lines (FHS and FHC) and 153 colorectal cancer (CRC) cell lines from GEO database (GSE59857); unpaired t test. G, mRNA expression of TPH1 in the human normal colorectal epithelial cell line NCM460 and four human colorectal cancer cell lines (SW480, SW620, HCT116, and HT29), as well as THP-1. H, mRNA expression of Tph1 in mouse primary CECs and mouse colorectal cancer cell line CT26, as well as iBMDMs. I, Protein expression of TPH1 in NCM460, SW480, SW620, HCT116, HT29, and THP-1 cells, as well as CECs, CT26 cells, and iBMDMs. J and K, 5-HT concentrations in supernatants of NCM460, SW480, SW620, HCT116, HT29, and THP-1 cells, as well as CECs, CT26 cells, and iBMDMs detected by ELISA. G–K, Data represent mean ± SD for at least three independent experiments; one-way ANOVA; N.D., not detected. **, P < 0.01.

Fig. 5 Feces from patients with functional constipation and comorbid psychiatric symptoms increased 5-HT production. (A) QGP-1 cells treated with fecal supernatant (fecal supernatant: medium = 1:5) for various times, and cell viability was assessed by CCK-8. (B, C) Expression of TPH1 gene and protein as detected using qRT-PCR and WB assay. (D) The 5-HT of cell culture medium supernatant was detected by ELISA