Angiogenin Antibody - #DF6449

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产品: Angiogenin 抗体
货号: DF6449
描述: Rabbit polyclonal antibody to Angiogenin
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
分子量: 17kDa; 17kD(Calculated).
蛋白号: P03950
RRID: AB_2838412

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Angiogenin Antibody detects endogenous levels of total Angiogenin.
RRID:
AB_2838412
引用格式: Affinity Biosciences Cat# DF6449, RRID:AB_2838412.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ALS9; AMYOTROPHIC LATERAL SCLEROSIS; ANG 1; ANG; ANG I; ANG1; ANGI; ANGI_HUMAN; Angiogenin; Angiogenin ribonuclease RNase A family, 5; Epididymis luminal protein 168; HEL168; MGC22466; MGC71966; Ribonuclease 5; Ribonuclease RNase A Family 5; RNase 5; RNASE4; RNASE5;

抗原和靶标

免疫原:
Uniprot:

P03950(ANGI_HUMAN) >>Visit HPA database.

基因/基因ID:
ANG(283)
表达:
P03950 ANGI_HUMAN:

Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.

描述:
Angiopoietins are a family of Tie receptor ligands. There are four angiopoietins discovered so far: angiopoietins 1, 2, 3 and 4 (Ang1, 2, 3, and 4) (1-3). Ang1 binds to the Tie-2 receptor and leads to its autophosphorylation and subsequent activation of downstream signaling pathways. It plays an important role in blood vessel formation, maturation and subsequent stabilization (1,4,5). Ang2 is an endothelium-specific growth factor that functions as an antagonist to Ang1, promotes vascular associated proinflammatory function, destabilizes quiescent endothelium, leads to vascular leakage and vascular destablization and remodeling (2,6,7). Ang2 is selectively expressed in many tumor tissues where, combined with other growth factors such as VEGF, it can promote vascular remodeling, angiogenesis and inflammation (7-9).
序列:
MVMGLGVLLLVFVLGLGLTPPTLAQDNSRYTHFLTQHYDAKPQGRDDRYCESIMRRRGLTSPCKDINTFIHGNKRSIKAICENKNGNPHRENLRISKSSFQVTTCKLHGGSPWPPCQYRATAGFRNVVVACENGLPVHLDQSIFRRP

研究背景

功能:

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.

细胞定位:

Cytoplasmic vesicle>Secretory vesicle lumen. Secreted. Nucleus. Nucleus>Nucleolus.
Note: Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA (PubMed:12051708).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.

亚基结构:

Homodimer. Interacts with and forms a tight 1:1 complex with RNH1. Dimerization of two such complexes may occur.

蛋白家族:

Belongs to the pancreatic ribonuclease family.

文献引用

1). Polyphenol-driven facile assembly of a nanosized acid fibroblast growth factor-containing coacervate accelerates the healing of diabetic wounds. Acta Biomaterialia, 2023 (PubMed: 36460288) [IF=9.4]
2). Inhibitory effect of miR‑182‑5p on retinal neovascularization by targeting angiogenin and BDNF. Molecular Medicine Reports, 2022 (PubMed: 34935052) [IF=3.4]

Application: WB    Species: Human    Sample: HRECs

Figure 1. OIR-induced suppression of miR-182-5p and increased expression of ANG and BDNF. (A) The potential binding sites of miR-182-5p on 3′-UTR of ANG and BDNF predicted by TargetScan. (B) The reverse transcription-quantitative PCR analysis showed miR-182-5p was inhibited in retinas of OIR mouse compared with normal mouse. (C) The mRNA levels of ANG and BDNF were increased in OIR mouse retina, compared with normal mouse. (D) The representative grey bands of ANG and BDNF by western blotting. (E) The quantitation demonstrated the enhanced protein levels of ANG and BDNF in OIR mouse retinas. Means ± SDs. **P<0.01 vs. the NC group (n=6). OIR, oxygen-induced retinopathy; ANG, angiogenin; BDNF, brain-derived neurotrophic factor; miR, microRNA; NC, negative control.
3). Menstrual Blood-Derived Endometrial Stem Cell Transplantation Improves Male Reproductive Dysfunction in T1D Mice by Enhancing Antioxidative Capacity. Reproductive sciences (Thousand Oaks, Calif.), 2024 (PubMed: 38396297) [IF=2.6]

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