AIF1/IBA1 Antibody - #DF6442

Figure 2 BDD exerts antidepressant effects by inhibiting the prefrontal Th17 cell-driven RORγt /Act1/TRAF6 pathway mediates microglial activation. A) Sucrose preference (%) in the SPT. B) Immobility time in the FST. C) Retention time in the open field center. D) Percentage of retention time in the open arm. E) Levels of IL-1β, IL-17A, and IL-10 in the mPFC of control or CUMS mice after treatment with saline or BDD. F) Representative flow cytometric analysis and statistical result of CD4+ Th17 cells contents within the mPFC. G) Fluorescence micrographs of DAPI (blue) and RORγt in the mPFC (×40 magnification, scale bar=10 µm). Quantitative analysis of RORγt, Iba1, iNOS, Arg-1, IL-17AR, Act1, and TRAF6 mRNAs H) level and protein expression of RORγt, Iba1, IL-17AR, Act1, and TRAF6 I) in the mPFC of each group of mice. GAPDH was set as the internal control. Data for individual animals are displayed as mean ± SEM (n=6 per/group). J) Immunofluorescence double staining of retinal sections with Iba1 (blue), IL-17AR (red), Act1 (red) or TRAF6 (red) and the ratio of IL-17AR+ Iba1+ / Iba1+ cells, Act1+ Iba1+ / Iba1+ cells and TRAF6+ Iba1+ / Iba1+ cells. Three non-overlapping fields of view were selected randomly in each slice under the fluorescence microscope. Arrows indicate the positive cells. Histogram is the quantification of the percentage of positive cells (n=3 per/group, 2 sections/animal). ** p

Fig. 5 Lateral ventricle administration of BMSC-exos inhibited the proliferation and activation of microglia in the hippocampus of mice injected with STZ. A Fluorescence detection of microglia marker IBA1 in hippocampus B Quantification of the number of IBA1 positive cells. C Microglia in different states (left: resting state; right: activated state); D The ratio of activated microglia to the total number of microglia. Data are presented as means ± SEM, with n = 3 in each group

Fig. 8 Effects of HRS treatment on protein expressions in septic rats 48 h after LPS challenge. A Representative images of protein expression levels by western blot analysis in each group. B–E Statistical representation of relative protein expressions of GFAP, IBA-1, BCL-2 and BAX; respectively. All data are presented as mean ± SD ****p < 0.0001, ***p < 0.001, **p < 0.005, *p < 0.05. HRS Hydrogen-rich saline, LPS Lipopolysaccharide, GFAP Glial fibrillary acidic protein, IBA-1 Ionised calcium binding adaptor molecule 1, BCL-2 B-cell lymphoma 2

Fig. 3. HXP reduced neuroinflammation in MCAO rats by promoting MCPIP1-mediated microglia M2 polarization. A. After reperfusion for 7 d, brain water contents were examined. B. Brain infract volume was detected by 2,3,5-Triphentltetrazolium chloride staining. C. Changes in IL-1β, IL-6, iNOS, and TNF-α were measured via ELISA assy. D. Representative images of histopathological analysis. Scale bar, 20 µm. E. Representative immunofluorescence staining for CD206 (red) and Iba1 (green) in brain tissues. Scale bar, 20 µm. F. The expression of MCPIP1, CD16, iNOS, CD206, Arg1, and PPARγ was detected using Western blotting assay. Data are represented as mean ± standard deviation (N = 5). * * P