Caspase 7 Antibody - #DF6441

Fig. 5. Combination GSK-LSD1 + AQB pro- motes apoptosis by targeting BBC3 in vitro. (A) mRNA levels of BBC3 measured by qPCR after treatment 100 µM GSK-LSD1, 40 µM AQB, or 100 µM GSK-LSD1 + 40 µM AQB for 48 h. (B) Relative BBC3 mRNA levels after treat- ment with 100 µM GSK-LSD1, 40 µM AQB, or their combination after 12, 24, 48, or 72 h. (C) Relative BBC3 mRNA levels measured by qPCR after treatment with indicated concen- trations of AQB or GSK-LSD1 for 48 h. (D) Combination treatment effects on BBC3 mRNA levels at varied concentrations. (E) Western blot analysis of PUMA, Caspase 3, Caspase 7 after treatment with indicated concentrations of AQB for 48 h. (F) Western blot analysis of PUMA, Caspase 3, Caspase 7 after treatment with 200 µM GSK-LSD1, 80 µM AQB, or 200 µM GSK-LSD1 + 80 µM AQB for 48 h. (G) Apoptosis detection after 48 h of treatment with 200 µM GSK-LSD1, 80 µM AQB, or 200 µM GSK-LSD1 + 80 µM AQB. (H) Immunofluorescence assay for PUMA expression after the treatment with 100 µM GSK-LSD1, 40 µM AQB, or 100 µM GSK-LSD1 + 40 µM AQB for 48 h. Data are represented as mean ± s.d.; n = 3 indepen- dent experiments. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05.

Fig. 2 Functional effects of lncRNA RP11-197K6.1 knockdown in CRC cells. A: Confirmation of lncRNA RP11-197K6.1 knockdown efficiency in HCT116 cells by RT-qPCR. B: Reduction in the proliferation of HCT116 and SW480 cells post-knockdown, as determined by proliferation assays. C: Increase in the apoptosis of HCT116 and SW480 cells following lncRNA RP11-197K6.1 knockdown, as measured by flow cytometry. D & E: Decrease in the migration and invasion of HCT116 and SW480 cells post-knockdown, as assessed by wound healing (scale = 200 μm) and Transwell assays (scale = 50 μm), respectively. 2 F: Changes in the levels of proteins related to the proliferation, apoptosis, and migration of HCT116 and SW480 cells after lncRNA RP11-197K6.1 knockdown, as analyzed by western blotting assay (**P 

FIGURE 2 CircSMOC1 promoted NSCLC cell growth and invasion and blocked cell apoptosis. (A) RT-qPCR analysis of the expression levels of circSMOC1 in various lung cancer cell lines. (B) RT-qPCR analysis of the expression levels of circSMOC1 after the transfection with circSMOC1 plasmids in A549 and H1299 or sh-circSMOC1 in PC-9 and 95D cells. (C) CCK-8 analysis of the cell viability after transfection with circSMOC1 plasmids in A549 and H1299 or sh-circSMOC1 in PC-9 and 95D cells. (D, F) Colony formation analysis of cell colony number after transfection with circSMOC1 plasmids in A549 and H1299 or sh-circSMOC1 in PC-9 and 95D cells. (E, G) Transwell analysis of cell invasion capabilities after transfection with circSMOC1 plasmids in A549 and H1299 or sh-circSMOC1 in PC-9 and 95D cells. (H) flow cytometry analysis of the effects of circSMOC1 overexpression or knockdown on cell apoptosis in NSCLC cells. (I) Western blotting analysis of the effects of circSMOC1 overexpression or knockdown on the protein levels of total or cleaved caspase -9, -7 and -3 in NSCLC cells. Data shown are the mean ± SEM of three experiments. *p