p21 Cip1 Antibody - #DF6423

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产品: p21 Cip1 抗体
货号: DF6423
描述: Rabbit polyclonal antibody to p21 Cip1
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Horse, Rabbit, Dog
蛋白号: P38936
RRID: AB_2838386

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
p21 Cip1 Antibody detects endogenous levels of total p21 Cip1.
RRID:
AB_2838386
引用格式: Affinity Biosciences Cat# DF6423, RRID:AB_2838386.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CAP20; CDK-interacting protein 1; CDKI; CDKN1; Cdkn1a; CDN1A_HUMAN; CIP1; Cyclin Dependent Kinase Inhibitor 1A; Cyclin-dependent kinase inhibitor 1; Cyclin-dependent kinase inhibitor 1A (P21); Cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA Synthesis Inhibitor; MDA-6; MDA6; Melanoma differentiation-associated protein 6; Melanoma differentiation-associated protein; p21; P21 protein; p21CIP1; p21Cip1/Waf1; p21WAF; PIC1; SDI1; SLC12A9; WAF1; Wild type p53 activated fragment 1 (WAF1); Wild type p53 activated fragment 1; Wildtype p53-activated fragment 1;

抗原和靶标

免疫原:

A synthesized peptide derived from human p21 Cip1, corresponding to a region within the internal amino acids.

基因/基因ID:
CDKN1A(1026)
描述:
The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S (1). However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2). The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3). Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A is active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4). Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation (5).

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Thyroid cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

文献引用

1). The NLRX1-SLC39A7 complex orchestrates mitochondrial dynamics and mitophagy to rejuvenate intervertebral disc by modulating mitochondrial Zn2+ trafficking. Autophagy, 2024 (PubMed: 37876250) [IF=14.6]
2). SYNJ2BP ameliorates intervertebral disc degeneration by facilitating mitochondria-associated endoplasmic reticulum membrane formation and mitochondrial Zn2+ homeostasis. Free radical biology & medicine, 2024 (PubMed: 38158052) [IF=7.1]

Application: WB    Species: human    Sample: NP cell

Fig. 1. MAM structure is impaired in degenerated disc. (A) Confocal analysis of TOMM20 and CANX with IF staining in human NP cells isolated from health or degenerated NP tissues, scale bar: 10 μm. (B) MAM structure analysis by TEM in human NP cells isolated from health or degenerated NP tissues, scale bar: 1 μm. (C, D) Protein expressions of cell senescence indicators (TP53, CDKN1A, CDKN2A) and extracellular matrix (COL2A1) in primary human NP cells isolated from health NP tissues with the treatment of TBHP, as determined by western blotting. (E-G) Cell senescence (SA-GLB1/β-gal staining) and cell proliferation (EdU incorporation) in primary human NP cells isolated from health NP tissues with the treatment of TBHP, scale bar: 100 μm (white light images), 200 μm (IF images). (H) Confocal analysis of TOMM20 and CANX with IF staining in human NP cells isolated from health NP tissues with the treatment of TBHP, scale bar: 10 μm. (I) MAM structure analysis by transmission electron microscopy (TEM) in human NP cells isolated from health NP tissues with the treatment of TBHP, scale bar: 1 μm. Data are represented as mean ± SD. *p 
3). Specnuezhenide Alleviates Senile Osteoporosis by Activating TGR5/FXR Signaling in Bone Marrow Mesenchymal Stem Cells and RANKL-Induced Osteoclasts. Drug design, development and therapy, 2025 (PubMed: 40066080) [IF=4.7]
4). Crocetin inhibits the proliferation, migration and TGF-β 2-induced epithelial-mesenchymal transition of retinal pigment epithelial cells . European Journal of Pharmacology, 2017 (PubMed: 28970011) [IF=4.2]

Application: WB    Species: human    Sample:

Figure 2. Crocetin induces cell cycle arrest and influences PCNA, p21, and p53 expression in RPE cells. (A) Cells were harvested after treatment with or without 50 and 100 μM crocetin for 24 h. DNA was stained with PI for flow cytometric analysis. The number of cells in G1 phase was significantly increased in the crocetin-treated group compared with that in the untreated group. (B) Cells were treated or without with 50, 100 and 200 μM crocetin for 48 h. Western blot analysis was used to evaluated the expression of PCNA, p21, p53 and the housekeeping protein β-actin. *P< 0.05 vs 0 μM crocetin, **P< 0.01 vs 0 μM crocetin. The data are presented as the mean ± S.D. (n = 3/group).
5). The role of cellular senescence in the pathogenesis of Rheumatoid Arthritis: Focus on IL-6 as a target gene. Cytokine, 2024 (PubMed: 39326197) [IF=3.7]
6). Nonlinear ageing gero-marker dynamics of transcriptomic profile during calcific aortic valve mouse modeling. Archives of gerontology and geriatrics, 2025 (PubMed: 39922128) [IF=3.5]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 1. Aortic function changes during ageing process in mouse model. (A) Representative images of PW doppler mode echocardiography of mice from each group. (B) Representative images of M-mode echocardiography of mice from each group. (C) Representative images of HE-stained cardiac tissues of mice from each group. (D) Representative images of SR-stained cardiac tissues of mice from each group through polarization microscope. (E) Pvel based on echocardiographic measurements, n = 3. (F) MPG based on echocardiographic measurements, n = 3. (G) AVD based on echocardiographic measurements, n = 3. (H) The area of collagenous fiber I and III measured according to SR staining, n = 3.
7). Impact of a high‑fat diet on intestinal stem cells and epithelial barrier function in middle‑aged female mice. Molecular Medicine Reports, 2020 (PubMed: 32016468) [IF=3.4]

Application: IHC    Species: Mice    Sample: apoptotic cells

Figure 6. Cell apoptosis in the small intestine was not affected by HFD. (A) Ileal sections stained for apoptotic cells (scale bar, 100 µm; magnification, ×20). Quantification of apoptotic cells among (B) villi and (C) crypt sections. (D) Expression of P21 in the small intestine, examined using immunohistochemistry (scale bars, 100 µm; magnification, ×40). Quantification of P21 positive cells as a percentage of the (E) crypts and (F) villi. (G) Immunohistochemical assay for P53 in the small intestine (scale bars, 100 µm; magnification, ×40). Quantification of P53 positive cells as a percentage of the (H) crypts and (I) villi. (J) P21 and (K) P53 mRNA expression levels in the small intestine. Data are presented as the mean ± standard deviation. HFD, high-fat diet; Con, control.
8). TRIM50 Inhibits Proliferation and Metastasis of Gastric Cancer via Promoting β-Catenin Degradation. Journal of Oncology, 2022 (PubMed: 36046365)

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