MIF Antibody - #DF6404

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产品: MIF 抗体
货号: DF6404
描述: Rabbit polyclonal antibody to MIF
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
蛋白号: P14174
RRID: AB_2838367

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
MIF Antibody detects endogenous levels of total MIF.
RRID:
AB_2838367
引用格式: Affinity Biosciences Cat# DF6404, RRID:AB_2838367.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

GIF; GLIF; Glycosylation inhibiting factor; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor (glycosylation-inhibiting factor); Macrophage migration inhibitory factor; MIF; MIF protein; MIF_HUMAN; MMIF; Phenylpyruvate tautomerase;

抗原和靶标

免疫原:

A synthesized peptide derived from human MIF, corresponding to a region within C-terminal amino acids.

基因/基因ID:
MIF(4282)
描述:
Macrophage migration inhibitory factor, known as MIF or glycosylation-inhibiting factor, is a secreted, homotrimeric, pro-inflammatory cytokine that modulates macrophage and T cell function and is an important regulator of host response to infection. MIF is expressed at sites of inflammation, which suggests that it plays a role in regulating macrophage function in host defense. MIF is produced by the pituitary gland and is found in monocytes, macrophages, differentiating immunological cells in the eye lens and brain, and fibroblasts. Elevated levels of MIF protein are detected in the plasma of patients with severe sepsis or septic shock, a condition where MIF influences endotoxic shock by enhancing the production of other inflammatory cytokines including tumor necrosis factor (TNF ), interleukin-1 (IL-1) and interferon- (IFN- ). MIF promotes the systemic inflammatory response by counter-regulating glucocorticoid-mediated inhibition of immune-cell activation and proinflammatory cytokine production. MIF may mediate tissue destruction through the induction of proteinases.

研究领域

· Metabolism > Amino acid metabolism > Tyrosine metabolism.

· Metabolism > Amino acid metabolism > Phenylalanine metabolism.

文献引用

1). Allyl isothiocyanate mitigates airway inflammation and constriction in a house dust mite-induced allergic asthma model via upregulation of tight junction proteins and the TRPA1 modulation. Biomedicine & Pharmacotherapy, 2023 (PubMed: 37634475) [IF=6.9]

Application: WB    Species: Mouse    Sample:

Fig. 3. Effect of AITC on airway inflammation in HDM-induced asthma mice. Treat with AITC significantly decreased the level of (A) HDM-specific IgE, (B) IL-4, (C) IL-5, (D) IL-13 and (E) MIF. (F) Histology image of H&E stained lung sections and (G) mean inflammatory index for each sample in the same vision area is a measure of the severity of inflammation ranging from 0 to 3. The upper and lower vision areas are magnified at 200 × and 400 ×, respectively. Data are expressed in mean ± SEM (n = 5–10). Significant difference presented as different symbol. *p 
2). MIF aggravates experimental autoimmune prostatitis through activation of the NLRP3 inflammasome via the PI3K/AKT pathway. International immunopharmacology, 2024 (PubMed: 39153310) [IF=4.8]
3). MIF regulates T helper 17 cell differentiation by activating the p38 MAPK signaling pathway to drive the pathogenesis of EAP. International immunopharmacology, 2025 (PubMed: 40440956) [IF=4.8]
4). Macrophage migration inhibitory factor in inflammasome formation and macrophage recruitment by cervical squamous cell carcinoma cells. Oncology letters, 2025 (PubMed: 39944790) [IF=2.5]
5). Screening of immunosuppressive factors for biomarkers of breast cancer malignancy phenotypes and subtype-specific targeted therapy. PeerJ, 2022 (PubMed: 31293831) [IF=2.3]

Application: IHC    Species: human    Sample: breast

Figure 7 Immunohistochemical detection of the expression of MIF and VEGFA in a breast cancer tissue microarray. (A, D) Negative expression (-) in cancer-adjacent normal breast tissue.
6). Screening of Immunosuppressive Factors for Identifying Breast Cancer Malignant Phenotypes Using mRNA Microarray Datasets. , 2022

Application: IHC    Species: Human    Sample: breast cancer

Figure 3 Immunohistochemical detection of MIF (Row 1) and VEGFA (Row 2) in breast cancer tissue microarray.(a) and (D), Negative expression in Cancer adjacent normal breast tissue. (b) and (e), Negative expression in Invasive ductal carcinoma. (c) and (f), Positive expression in Invasive ductal carcinoma.
7). Migration Inhibition Factor Secreted by Peripheral Blood Memory B Cells Binding to CD74-CD44 Receptor Complex Drives Macrophage Behavior in Alzheimer's Disease. American journal of Alzheimer's disease and other dementias, 2024 (PubMed: 38491918)

Application: IF/ICC    Species: human    Sample:

Figure 3. Memory B Cells - Secreted MIF Conducts a Major Signal between Memory B Cells and Macrophages in AD. (A) VIN plot showing that MIF is mainly produced by Memory B Cells, and Macrophages significantly express the CD74-CD44 receptor complex. (B) Immunofluorescent imaging analysis demonstrating MIF expression on Memory B Cells. (C) Immunofluorescent imaging analysis revealing CD74-CD44 receptor complex expression on Macrophages. (D) The immunofluorescence semi-quantitative expression of macrophages and memory B cells. ***P < .001. (E) Double immunofluorescent staining showing co-localization of MIF and CD74-CD44 receptor complex proteins.

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