HO-1 Antibody - #DF6391

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产品: HO-1 抗体
货号: DF6391
描述: Rabbit polyclonal antibody to HO-1
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
蛋白号: P09601
RRID: AB_2838354

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
HO-1 Antibody detects endogenous levels of total HO-1.
RRID:
AB_2838354
引用格式: Affinity Biosciences Cat# DF6391, RRID:AB_2838354.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

32 kD; bK286B10; D8Wsu38e; heat shock protein 32 kD; heat shock protein 32kD; Heat shock protein; Heme oxygenase (decycling) 1; Heme oxygenase 1; Hemox; HMOX 1; Hmox; Hmox1; HMOX1_HUMAN; HO 1; HO; HO-1; HO1; Hsp32;

抗原和靶标

免疫原:

A synthesized peptide derived from human HMOX1, corresponding to a region within the internal amino acids.

基因/基因ID:
HMOX1(3162)
描述:
Hemeoxygenase (HO) is the rate-limiting enzyme in the catabolism of heme that results in the release of carbon monoxide, iron, and biliverdin (1). The products of this enzymatic reaction play important biological roles in antioxidant, anti-inflammatory and cytoprotective functions (2). Hemeoxygenase comprises two isozymes, including the constitutively expressed HO-2 isozyme and the inducible HO-1 isozyme (3). Inducible HO-1 is expressed as an adaptive response to several stimuli, including heme, metals, and hormones (4). The induction of HO-1 has been implicated in numerous disease states, such as transplant rejection, hypertension, atherosclerosis, Alzheimer disease, endotoxic shock, diabetes, inflammation, and neurological disorders (1,5).

研究领域

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Metabolism > Metabolism of cofactors and vitamins > Porphyrin and chlorophyll metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Digestive system > Mineral absorption.

文献引用

1). Tetrahydrocurcumin protects against nonalcoholic fatty liver disease by improving lipid metabolism and redox homeostasis. Journal of Functional Foods, 2022 [IF=3.8]

Application: WB    Species: Human    Sample: HepG2 cells

Fig. 6. Effect of tetrahydrocurcumin (THC) on lipid content and expression of the NRF2 pathway in HepG2 cells. HepG2 cells were treated with 0.2 mM sodium oleate (OA) for 24 h, and then with THC for 48 h. Oil red O was used to determine the (A) Intracellular lipid content. RT-qPCR and Western blot were used to analyze the expression of NRF2 signaling pathway. (B) Relative expression of NRF2, NQO1 and HO-1 mRNA; (C) Relative expression of GSTA1, GCLC and GCLM mRNA; (D) Relative expression of NRF2 protein; (E) Relative expression of NQO1 protein; (F) Relative expression of HO-1 protein. Different letters (a-d) between groups indicate significant differences
2). Attenuation of blue light-induced photo-oxidative stress through inhibition of NF-κB and MAPK signaling pathways, and activation of Nrf2 signaling pathway by djulis and its bioactive compounds. Journal of Functional Foods, 2023 [IF=3.8]
3). Hyperoside Alleviates Helicobacter pylori-Induced Gastric Epithelial Cell Injury by Regulating Nrf2/HO-1 Signaling. Polish journal of microbiology, 2025 (PubMed: 40146790) [IF=2.0]

Application: WB    Species: human    Sample: GES-1 cells

Fig. 4. HYP activates the Nrf2/HO-1 signaling in Helicobacter pylori-infected GES-1 cells. GES-1 cells were pre-treated with HYP (80 µM) for 4 h, followed by H. pylori infection for 24 h. A–D) Determination of Nrf2, HO-1, and NQO1 protein levels in GES-1 cells by western blotting. ***p < 0.001 versus control; ###p < 0.001 versus H. pylori; E) representative immunofluorescence staining images showing Nrf2 expression (green) in GES-1 cells. Nuclei were stained with DAPI (blue); F–I) estimation of Nrf2, HO-1, and NQO1 protein levels in GES-1 cells that were pre-treated with HYP (80 µM) and ML385 (5 µM) for 4 h before infection with H. pylori through western blotting. **p < 0.01, ***p < 0.001 vs. H. pylori; ###p < 0.001 vs. H. pylori + HYP.
4). 紅藜與其活性成分對LED光源誘發視網膜傷害之保護效應. 計畫編號：MOST110-2320-B041-001-MY2, 2022

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