GHRL Antibody - #DF6389

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产品: GHRL 抗体
货号: DF6389
描述: Rabbit polyclonal antibody to GHRL
应用: WB IHC
文献验证: IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep
蛋白号: Q9UBU3
RRID: AB_2838352

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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实验方案
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IHC Protocol
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
GHRL Antibody detects endogenous levels of total GHRL.
RRID:
AB_2838352
引用格式: Affinity Biosciences Cat# DF6389, RRID:AB_2838352.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Appetite regulating hormone; Ghrelin 27; Ghrelin 28; Ghrelin and obestatin prepropeptide; Ghrelin; Ghrelin/obestatin prepropeptide; ghrl; GHRL_HUMAN; Growth hormone releasing peptide; Growth hormone secretagogue; Growth hormone-releasing peptide; M46 protein; Motilin related peptide; Motilin-related peptide; MTLRP; Obestatin; Protein M46;

抗原和靶标

免疫原:

A synthesized peptide derived from human GHRL, corresponding to a region within C-terminal amino acids.

基因/基因ID:
GHRL(51738)
描述:
This gene encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes. Alternative promoters and alternative splicing result in multiple transcript variants, some of which encode different protein isoforms and some of which do not encode a protein but may regulate the ghrelin-obestatin preproprotein expression. In addition, antisense transcripts for this gene have been identified and may also function in regulation of the ghrelin-obestatin preproprotein expression. [provided by RefSeq, Oct 2008]

研究领域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

文献引用

1). Maternal acrylamide exposure changes intestinal epithelium, immunolocalization of leptin and ghrelin and their receptors, and gut barrier in weaned offspring. Scientific Reports, 2023 (PubMed: 37355724) [IF=3.8]

Application: IHC    Species: Rat    Sample:

Figure 3 Quantitative analysis of the intensity of ghrelin IR in (A) villi and (B) crypts of small intestine segments, measured by comparison of the pixel brightness value in the microscopic images converted to 8-bit, grey-scale images (the higher the pixel value, the higher the intensity of the IR). (C) Representative images of the ghrelin IR in sections of proximal jejunum; inset: antibody control. Quantitative analysis of the intensity of ghrelin receptor IR in (D) villi and (E) crypts of small intestine segments, measured by comparison of the optical density (OD) of the microscopic images converted to 8-bit, grey-scale images. (F) Representative images of the ghrelin IR in sections of duodenum. (C), (F): All scale bars represent 100 µm. (A), (B), (D), (E): Bar plots show lsmeans value and standard deviation (whiskers). A p-value range was attributed above plots when two groups exhibit significant differences: *p value 
2). Basal Intestinal Morphology, Immunolocalization of Leptin and Ghrelin and Their Receptors in Newborn Wistar Rats after Prenatal Exposure to Fumonisins. Animals, 2023 (PubMed: 37174575) [IF=2.7]

Application: IHC    Species: Rat    Sample:

Figure 2 Representative images and quantitative analysis of the intensity of ghrelin, ghrelin receptor, leptin, and leptin receptor immunoreaction (IR) in crypts and villi of the duodenum of female newborn rat offspring after maternal exposure to fumonisins (FBs) at the doses of 0, 60, or 90 mg/kg b.w. Bottom isotype row shows IHC IR control incubated without a primary antibody. All of the images were taken at the same magnification; scale bars represent 50 μm. Bar charts: intensity of immunoreaction (IR) to specific antibody was measured in crypts and villi using optical density (OD) measurements in the microscopic images. Results are presented as mean and SD. Statistically significant differences between groups are indicated by

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