MMP1 Antibody - #DF6325

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产品: MMP1 抗体
货号: DF6325
描述: Rabbit polyclonal antibody to MMP1
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: P03956
RRID: AB_2838289

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Rat
克隆:
Polyclonal
特异性:
MMP1 Antibody detects endogenous levels of total MMP1.
RRID:
AB_2838289
引用格式: Affinity Biosciences Cat# DF6325, RRID:AB_2838289.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

27 kDa interstitial collagenase; CLG; CLGN; collagenase, fibroblast; collagenase, interstitial; Fibroblast collagenase; Interstitial collagenase; Matrix metallopeptidase 1 (interstitial collagenase); Matrix metalloprotease 1; Matrix Metalloproteinase 1; Matrix metalloproteinase-1; MMP 1; MMP-1; MMP1; MMP1_HUMAN; OTTHUMP00000045866;

抗原和靶标

免疫原:

A synthesized peptide derived from human MMP1, corresponding to a region within C-terminal amino acids.

基因/基因ID:
MMP1(4312)
描述:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

研究领域

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

文献引用

1). Downregulated cytotoxic CD8+ T-cell identifies with the NKG2A-soluble HLA-E axis as a predictive biomarker and potential therapeutic target in keloids. Cellular & molecular immunology, 2022 (PubMed: 35039632) [IF=21.8]
2). A non-retinol retinoic acid receptor-γ (RAR-γ/NR1B3) selective agonist, tectorigenin, can effectively inhibit the ultraviolet A-induced skin damage. British journal of pharmacology, 2022 (PubMed: 35731978) [IF=6.8]
3). Timosaponin B-II alleviates osteoarthritis-related inflammation and extracellular matrix degradation through inhibition of mitogen-activated protein kinases and nuclear factor-κB pathways in vitro. Bioengineered, 2022 (PubMed: 35094658) [IF=4.2]

Application: WB    Species: Human    Sample: SW1353 cells and chondrocytes

Figure 4. TB-II inhibited the production of cartilage degrading enzymes in IL-1β-treated SW1353 cells and chondrocytes. The mRNA expression of (a) MMP-1, (b) MMP-3 and (c) MMP-13 were detected by qRT-PCR. (d-g) The protein level of MMP-1, MMP-3, and MMP-13 were detected by Western blot. GAPDH was conducted as a loading control. One-way ANOVA, ##p < 0.01, compared with control cells; $p < 0.05, $$p < 0.01, ns: not-significant, compared with IL-1β-treated cells.
4). Deciphering the pharmacological mechanisms of Fraxini Cortex for ulcerative colitis treatment based on network pharmacology and in vivo studies. BMC Complementary Medicine and Therapies, 2023 (PubMed: 37161415) [IF=3.9]

Application: WB    Species: Mouse    Sample:

Fig. 11 FC regulated the expression of key target genes. The protein expression levels of (A-B) IL-1β, COX2, (C-D) IL-17, RORγt, and (E-F) MMP1, MMP3 and MMP9 were measured by western blot. Data are shown as the mean ± SD, #P 
5). RUNX2 facilitates aggressiveness and chemoresistance of triple negative breast cancer cells via activating MMP1. Frontiers in oncology, 2022 (PubMed: 36483054) [IF=3.5]

Application: WB    Species: Mouse    Sample:

Figure 8 MMP1 and RUNX2 showed positively correlation in relative mRNA and protein expression. (A) qRT-PCR analysis of the expression of RUNX2 and MMP1 in various breast cancer cell lines. (B, C) qRT-PCR and western-blotting analyses of RUNX2 (B) and MMP1 (C) expression in MDA-MB-231-Re cells or MDA-MB-231-Pa cells. (D, E) qRT-PCR analysis of MMP1 expression in RUNX2 knockdown MDA-MB-231-Re and MDA-MB-231 cells (D) as well as the SUM-149 with RUNX2 overexpression (E). (F) RUNX2 and MMP1 expression of tumor sections from each group were determined by immunohistochemistry. Scale bar =50 µm. *P

Application: IHC    Species: Mouse    Sample:

Figure 8 MMP1 and RUNX2 showed positively correlation in relative mRNA and protein expression. (A) qRT-PCR analysis of the expression of RUNX2 and MMP1 in various breast cancer cell lines. (B, C) qRT-PCR and western-blotting analyses of RUNX2 (B) and MMP1 (C) expression in MDA-MB-231-Re cells or MDA-MB-231-Pa cells. (D, E) qRT-PCR analysis of MMP1 expression in RUNX2 knockdown MDA-MB-231-Re and MDA-MB-231 cells (D) as well as the SUM-149 with RUNX2 overexpression (E). (F) RUNX2 and MMP1 expression of tumor sections from each group were determined by immunohistochemistry. Scale bar =50 µm. *P
6). Ubiquitin-specific protease 49 attenuates IL-1β-induced rat primary chondrocyte apoptosis by facilitating Axin deubiquitination and subsequent Wnt/β-catenin signaling cascade inhibition. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2020 (PubMed: 32737772) [IF=3.5]

Application: WB    Species: rat    Sample: chondrocytes

FIGURE 2 |e) Western blot analysis (right panel) and band intensity analysis (left panel) of the protein levels of Survivin, Mmp1, and Mmp13 in Control+Vehicle, Vector+IL-1β, and oeUSP49 +IL-1β rat chondro-cytes. ***P<0.001 versus the Vector or Control+Vehicle group; ## and ### indicate P values less than 0.01 and 0.001,respectively, versus the Vec-tor+IL-1β group

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