PML Antibody - #DF6318

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产品: PML 抗体
货号: DF6318
描述: Rabbit polyclonal antibody to PML
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
蛋白号: P29590
RRID: AB_2838284

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
PML Antibody detects endogenous levels of total PML.
RRID:
AB_2838284
引用格式: Affinity Biosciences Cat# DF6318, RRID:AB_2838284.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Acure promyelocytic leukemia, inducer of; MYL; Pml; PML_HUMAN; PP8675; Probable transcription factor PML; Promyelocytic leukemia; Promyelocytic leukemia inducer of; Promyelocytic leukemia protein; Protein PML; RING finger protein 71; RNF 71; RNF71; TRIM 19; Tripartite motif protein TRIM19; Tripartite motif-containing protein 19;

抗原和靶标

免疫原:

A synthesized peptide derived from human PML, corresponding to a region within N-terminal amino acids.

基因/基因ID:
PML(5371)
描述:
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

研究领域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

文献引用

1). PML Regulated HIF1AN Ubiquitination and Activated PI3K/AKT Pathway to Promote Bone Marrow Mesenchymal Stem Cells Osteogenic Differentiation. International journal of stem cells, 2025 (PubMed: 40059075) [IF=2.5]
2). Autophagy and Ubiquitin-Mediated Proteolytic Degradation of PML/Rarα Fusion Protein in Matrine-Induced Differentiation Sensitivity Recovery of ATRA-Resistant APL (NB4-LR1) Cells: in Vitro and in Vivo Studies. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2018 (PubMed: 30114705) [IF=2.5]

Application: WB    Species: mouse    Sample: NB4-LR1 cells

Fig. 1.| MAT promotes the differentiation and induces the degradation of PML-RARα protein in NB4-LR1 cells in vitro. NB4 and NB4-LR1 cells were treated respectively with solvent, ATRA (1 μmol/L), MAT (0.1 mmol/L), ATRA combined with MAT, ATRA combined with Rapamycin (100nmol/L, autophagy activator), ATRA combined with STI571 (5 μmol/L, RARα ubiquitin stabilizer), MG132 (1 μmol/L, proteasome inhibitor), ATRA combined with MG132,MAT combined with MG132, ATRA combined with MAT and MG132, ATRA combined with MAT and hydroxychloroquine (HCQ, 16 μmol/L, autophagy inhibitor) for 72 h. (ch) Western-blot analysis of the PML-RARα fusion protein and PML protein, and quantitation were analyzed using Image J 1.46r software. Relative aboundances of the proteins were expressed relative to β-actin, which was set at 1.00.

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