Rab5A Antibody - #DF6314

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产品: Rab5A 抗体
货号: DF6314
描述: Rabbit polyclonal antibody to Rab5A
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Bovine, Horse, Rabbit, Dog, Chicken
蛋白号: P20339
RRID: AB_2838280

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Rab5A Antibody detects endogenous levels of total Rab5A.
RRID:
AB_2838280
引用格式: Affinity Biosciences Cat# DF6314, RRID:AB_2838280.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

RAB 5; RAB 5A; RAB5A; RAB5A member RAS oncogene family; RAB5A_HUMAN; RAS associated protein RAB5A; Ras related protein Rab 5A; Ras-related protein Rab-5A;

抗原和靶标

免疫原:

A synthesized peptide derived from human Rab5A, corresponding to a region within C-terminal amino acids.

基因/基因ID:
RAB5A(5868)
描述:
Rab5 is a member of the Ras superfamily of small Rab GTPases. Rab5 is localized at the plasma membrane and early endosomes and functions as a key regulator of vesicular trafficking during early endocytosis (1). The conformational change between Rab5 GTP/GDP states is essential for its biological function as a rate limiting regulator at multiple steps during endocytosis (1,2). Rab5 exerts its function by interacting with several Rab5-specific effectors (1-3). These proteins form complexes with Rab5 on a specialized Rab domain of the endosome and promote recycling of Rab5-cargo targets between endosome and the plasma membrane.

研究领域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

文献引用

1). Identification of Key Genes Associated with Endothelial Cell Dysfunction in Atherosclerosis Using Multiple Bioinformatics Tools. Biomed Research International, 2022 (PubMed: 35059464) [IF=2.6]

Application: WB    Species: Human    Sample: HCAECs

Figure 8 (a) RAB5A overexpression and shRNA plasmid transfection HCAECs in vitro. There was no significant difference in transfection efficiency between two groups (28.7% and 27.3%), and the green fluorescence carried by the plasmid itself can be seen. (b, c) The results of measuring the expression of RAB5A protein after transfection are shown in (b). Relative quantitative comparison of western blot analyses in (C). ∗p < 0.05, compared to the HCAEC group; #p < 0.05, compared to the HCAEC-RAB5A overexpression group.

Application: IF/ICC    Species: Human    Sample: HCAECs

Figure 10 (a, d) Wound healing assay in all groups. (a) Migration distance of HCAECs in different conditions. (d) Relative quantitative comparison of healing speed analyses mentioned in (a). (b, e) Transwell migration assay in all groups. (b) Representative photomicrographs showing migrating cells in different conditions. (e) Quantification of number of migrating cells demonstrated that overexpression of RAB5A promoted HCAEC migration function (n = 6/group). (c, f) Tube formation assay. (c) Representative photomicrographs showing tube formation of HCAECs in different conditions. (f) Quantification of HCAEC tube junction numbers demonstrated that overexpression of RAB5A ameliorated HCAECs to form capillary-like structures (n = 6/group). ∗p < 0.05, compared to the HCAEC group; #p < 0.05, compared to the HCAEC-RAB5A overexpression group.

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