TMS1/ASC Antibody - #DF6304

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产品: TMS1/ASC 抗体
货号: DF6304
描述: Rabbit polyclonal antibody to TMS1/ASC
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit
蛋白ID: Q9ULZ3
RRID: AB_2838270

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#DF6304-BP

PYCARD Peptide
相关下载
抗原肽/阻断肽MS报告
抗原肽/阻断肽HPLC报告
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
PYCARD Antibody detects endogenous levels of total TMS1/ASC.
RRID:
AB_2838270
引用格式: Affinity Biosciences Cat# DF6304, RRID:AB_2838270.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Apoptosis associated speck like protein containing a CARD; Apoptosis-associated speck-like protein containing a CARD; ASC; ASC_HUMAN; CARD 5; CARD5; Caspase recruitment domain containing protein 5; Caspase recruitment domain protein 5; Caspase recruitment domain-containing protein 5; hASC; MGC10332; PYCARD; PYD and CARD domain containing; PYD and CARD domain containing protein; PYD and CARD domain-containing protein; Target of methylation induced silencing 1; Target of methylation-induced silencing 1; TMS 1; TMS; TMS1;

抗原和靶标

免疫原:

A synthesized peptide derived from human TMS1/ASC, corresponding to a region within N-terminal amino acids.

基因/基因ID:
PYCARD(29108)
描述:
TMS1 (target of methylation-induced silencing)/ASC (apoptosis-associated speck-like protein containing a CARD), also referred to as PYCARD and CARD5, is a 22-kDa pro-apoptotic protein containing an N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD) (1-2). The TMS1 gene was originally found to be aberrantly methylated and silenced in breast cancer cells (2), and has since been found to be silenced in a number of other cancers, including ovarian cancer (3), glioblastoma (4), melanoma (5), gastric cancer (6), lung cancer (7), and prostate cancer (8). Expression of TMS1 can be induced by pro-apoptotic/inflammatory stimuli (9). During apoptosis TMS1 is re-distributed from the cytosol to the mitochondria and associates with mitochondrial Bax to trigger cytochrome c release and subsequent apoptosis (10). TMS1 has also been found to be a critical component of inflammatory signaling where it associates with and activates caspase-1 in response to pro-inflammatory signals (11).

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

文献引用

1). Gut microbial metabolite trimethylamine N-oxide aggravates GVHD by inducing M1 macrophage polarization in mice. BLOOD, 2020 (PubMed: 32291445) [IF=21.0]

Application: IF/ICC    Species: mouse    Sample: BMDMs

Figure 5.| TMAO enhanced M1 polarization via activating NLRP3 inflammasome. (A) Representative immunofluorescence staining of NLRP3 (green: Alexa Fluor 488), ASC (red: Alexa Fluor 594), DAPI (blue) on BMDMs after TMAO (300M) stimulation for 24hrs. Scale bar=5μm.
2). Dnmt1 Alleviates S1PR1-Mediated Pyroptosis after Spinal Cord Injury through Regulating Pon3 Expression. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025 (PubMed: 40884250) [IF=15.1]

Application: WB    Species: Rat    Sample:

Figure 3 Overexpression of Pon3 Attenuates Pyroptosis and Promotes Autophagy after SCI. A) Experimental timeline: Intraspinal injection of Pon3-overexpressing virus was performed 7 days before SCI, followed by Western blot and immunofluorescence analyses at 3 days after injury. B-C) Western blot analysis of pyroptosis-related proteins in spinal cord tissue at 3 days after SCI and their quantitative analysis (n = 3). D–F) Double immunofluorescence staining of Neun (green) with Caspase1 (red) or GSDMD (red) at 3 days after SCI, with quantitative analysis of relative fluorescence intensity (n = 3; scale bar: 100 µm in main panels; 20 µm in enlarged insets). G,H) Western blot analysis of autophagy-related proteins and their quantitative analysis at 3 days after SCI (n = 3). I,J) Double immunofluorescence staining of Neun (green) with P62 (red) and quantitative analysis (n = 3; scale bar: 100 µm in main panels; 20 µm in enlarged insets). Data are presented as mean ± SEM. Significance was determined by one-way or two-way ANOVA, followed by Tukey's multiple comparisons test.
3). Inhibition of macrophage inflammasome assembly and pyroptosis with GC-1 ameliorates acute lung injury. Theranostics, 2025 (PubMed: 39990234) [IF=12.4]
4). Antibiotic cocktail-induced changes in gut microbiota drive alteration of bile acid metabolism to restrain Th17 differentiation through the FXR-NLRP3 axis. Gut microbes, 2025 (PubMed: 41305918) [IF=12.2]

Application: WB    Species: Mouse    Sample:

Figure 6. DCA initiates the NLRP3‒IL17A pathway to promote Th17 differentiation (A) Feeding schedule for EAP mice treated with ABX, ABX + DCA or ABX + DCA + MCC950. (B) Assessment of pelvic pain in mice using von Frey filaments. (C) H&E staining revealing alterations in prostate tissue. The red arrow indicates the invasion of inflammatory cells. Inflammation score assessment in the ABX, ABX + DCA and ABX + DCA + MCC950 groups. (D) Relative level of RORγt mRNA in prostate tissue from ABX, ABX + DCA and ABX + DCA + MCC950 mice. (E, F) The percentages of CD4+ IL-17A + and CD4+ RORγt + cells in the spleen of ABX, ABX + DCA and ABX + DCA + MCC950 mice. (G, H) Sorted naïve CD4+ T cells were activated for 5 days under Th17 cell differentiation conditions without DCA (media group), with DCA (media + DCA group) or with DCA + MCC950 (media + DCA + MCC950 group). Representative images and analysis of FCM staining for CD4+ IL-17A + cells and CD4+ RORγt + cells in the three groups. (I) Western blot analysis of NLRP3-IL17A-related proteins in the media, media + DCA, and media + DCA + MCC950 groups (n = 3). (J) IHC of NLRP3-IL17A-related proteins in prostate tissue from ABX, ABX + DCA and ABX + DCA + MCC950 mice. (K) ChIP‒qPCR was used to determine the binding status of FXR at three sites in the NLRP3 promoter. (L) Dual-luciferase assay showing the interaction between FXR and NLRP3. (n = 3–5; *, P 
5). Overexpression of NAG-1/GDF15 prevents hepatic steatosis through inhibiting oxidative stress-mediated dsDNA release and AIM2 inflammasome activation. Redox Biology, 2022 (PubMed: 35504134) [IF=10.7]
6). 1,25-Dihydroxyvitamin D3 protects against placental inflammation by suppressing NLRP3-mediated IL-1β production via Nrf2 signaling pathway in preeclampsia. Metabolism: clinical and experimental, 2025 (PubMed: 39488297) [IF=9.8]
7). Vagus nerve stimulation alleviates S. aureus-induced mastitis by regulating gut microbiota S24-7-PPARγ and NF-ΚB/NLRP3 signaling in mice. Journal of neuroinflammation, 2025 (PubMed: 41430282) [IF=9.3]
8). Lactobacillus rhamnosus GG ameliorates triptolide-induced liver injury through modulation of the bile acid-FXR axis. Pharmacological research, 2024 (PubMed: 38908615) [IF=9.1]
9). DanShen Decoction targets miR-93-5p to provide protection against MI/RI by regulating the TXNIP/NLRP3/Caspase-1 signaling pathway. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 39547100) [IF=8.3]
10). Inhibition of IGF2BP1 attenuates renal injury and inflammation by alleviating m6A modifications and E2F1/MIF pathway. International Journal of Biological Sciences, 2023 (PubMed: 36632449) [IF=8.2]
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