产品: RRM1 抗体
货号: DF6286
描述: Rabbit polyclonal antibody to RRM1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 90kDa; 90kD(Calculated).
蛋白号: P23921
RRID: AB_2838252

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(90%)
克隆:
Polyclonal
特异性:
RRM1 Antibody detects endogenous levels of total RRM1.
RRID:
AB_2838252
引用格式: Affinity Biosciences Cat# DF6286, RRID:AB_2838252.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

R1; Ribonucleoside diphosphate reductase large subunit; Ribonucleoside diphosphate reductase M1 chain; Ribonucleoside diphosphate reductase subunit M1; Ribonucleoside reductase, large subunit; Ribonucleoside-diphosphate reductase large subunit; Ribonucleoside-diphosphate reductase subunit M1; Ribonucleotide reductase large chain; Ribonucleotide reductase large subunit; Ribonucleotide reductase M1; Ribonucleotide reductase M1 polypeptide; Ribonucleotide reductase R1 subunit; Ribonucleotide reductase, M1 subunit; RIR 1; RIR1; RIR1_HUMAN; RR 1; RR1; RRM 1; RRM1;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
Ribonucleotide reductase catalyzes the rate-limiting step in the synthesis of deoxynucleotide triphosphates (dNTPs). The regulatory M1 subunit (RRM1) is present throughout the cell division cycle, but downregulated in quiescent cells (1). Research studies have demonstrated that RRM1 is involved in carcinogenesis and tumor progression, and its expression is correlated with resistance to chemotherapy in non-small cell lung cancer (2-4).
序列:
MHVIKRDGRQERVMFDKITSRIQKLCYGLNMDFVDPAQITMKVIQGLYSGVTTVELDTLAAETAATLTTKHPDYAILAARIAVSNLHKETKKVFSDVMEDLYNYINPHNGKHSPMVAKSTLDIVLANKDRLNSAIIYDRDFSYNYFGFKTLERSYLLKINGKVAERPQHMLMRVSVGIHKEDIDAAIETYNLLSERWFTHASPTLFNAGTNRPQLSSCFLLSMKDDSIEGIYDTLKQCALISKSAGGIGVAVSCIRATGSYIAGTNGNSNGLVPMLRVYNNTARYVDQGGNKRPGAFAIYLEPWHLDIFEFLDLKKNTGKEEQRARDLFFALWIPDLFMKRVETNQDWSLMCPNECPGLDEVWGEEFEKLYASYEKQGRVRKVVKAQQLWYAIIESQTETGTPYMLYKDSCNRKSNQQNLGTIKCSNLCTEIVEYTSKDEVAVCNLASLALNMYVTSEHTYDFKKLAEVTKVVVRNLNKIIDINYYPVPEACLSNKRHRPIGIGVQGLADAFILMRYPFESAEAQLLNKQIFETIYYGALEASCDLAKEQGPYETYEGSPVSKGILQYDMWNVTPTDLWDWKVLKEKIAKYGIRNSLLIAPMPTASTAQILGNNESIEPYTSNIYTRRVLSGEFQIVNPHLLKDLTERGLWHEEMKNQIIACNGSIQSIPEIPDDLKQLYKTVWEISQKTVLKMAAERGAFIDQSQSLNIHIAEPNYGKLTSMHFYGWKQGLKTGMYYLRTRPAANPIQFTLNKEKLKDKEKVSKEEEEKERNTAAMVCSLENRDECLMCGS

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
90
Zebrafish
80
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P23921 作为底物

Site PTM Type Enzyme
K5 Ubiquitination
K17 Acetylation
K17 Ubiquitination
K24 Ubiquitination
K70 Ubiquitination
Y74 Phosphorylation
R80 Methylation
K88 Ubiquitination
K92 Ubiquitination
Y102 Phosphorylation
Y104 Phosphorylation
K111 Ubiquitination
K118 Ubiquitination
S119 Phosphorylation
K128 Ubiquitination
S133 Phosphorylation
S142 Phosphorylation
K149 Ubiquitination
K158 Ubiquitination
K162 Ubiquitination
K180 Ubiquitination
Y232 Phosphorylation
K236 Ubiquitination
K243 Ubiquitination
S253 Phosphorylation
K320 Methylation
K320 Ubiquitination
K369 Ubiquitination
K376 Acetylation
K376 Ubiquitination
K414 Ubiquitination
S415 Phosphorylation
T422 Phosphorylation
K424 Ubiquitination
K464 Ubiquitination
K465 Ubiquitination
K471 Ubiquitination
K479 Ubiquitination
Y485 Phosphorylation
Y486 Phosphorylation
K496 Acetylation
K496 Ubiquitination
K548 Ubiquitination
Y553 Phosphorylation
T555 Phosphorylation
Y556 Phosphorylation
S559 Phosphorylation
K563 Ubiquitination
K582 Ubiquitination
K587 Ubiquitination
K590 Ubiquitination
T604 Phosphorylation
S631 Phosphorylation
K643 Ubiquitination
K656 Ubiquitination
K677 Ubiquitination
K681 Ubiquitination
K689 Ubiquitination
K693 Ubiquitination
Y726 Phosphorylation
K729 Ubiquitination
K733 Ubiquitination
Y738 Phosphorylation
T751 Phosphorylation
K754 Ubiquitination
K762 Ubiquitination
K765 Ubiquitination
K770 Ubiquitination
T774 Phosphorylation
S780 Phosphorylation
S792 Phosphorylation

研究背景

功能:

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Heterodimer of a large and a small subunit. Heterodimer with small subunit RRM2 or RRM2B. The heterodimer with RRM2 has higher catalytic activity than the heterodimer with RRM2B. Interacts with AHCYL1 which inhibits its activity.

蛋白家族:

Belongs to the ribonucleoside diphosphate reductase large chain family.

研究领域

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Xia et al. Gambogic acid sensitizes gemcitabine efficacy in pancreatic cancer by reducing the expression of ribonucleotide reductase subunit-M2 (RRM2). Journal of Experimental & Clinical Cancer Research 2017 Aug 10;36(1):107 (PubMed: 28797284) [IF=11.3]

Application: WB    Species: human    Sample:


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