TCF4 Antibody - #DF6275

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产品: TCF4 抗体
货号: DF6275
描述: Rabbit polyclonal antibody to TCF4
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 71kDa; 71kD(Calculated).
蛋白号: P15884
RRID: AB_2838241

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
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实验方案
WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Bovine(100%), Horse(%), Sheep(%), Rabbit(%), Dog(%)
克隆:
Polyclonal
特异性:
TCF4 Antibody detects endogenous levels of total TCF4.
RRID:
AB_2838241
引用格式: Affinity Biosciences Cat# DF6275, RRID:AB_2838241.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

bHLHb19; Class B basic helix-loop-helix protein 19; E2 2; FECD3; Immunoglobulin transcription factor 2; ITF 2; ITF-2; ITF2; ITF2_HUMAN; MGC149723; MGC149724; PTHS; SEF 2; SEF-2; SEF2 1; SEF2 1A; SEF2 1B; SEF2 1D; SEF2; SL3 3 enhancer factor 2; SL3-3 enhancer factor 2; TCF 4; TCF-4; TCF4; Transcription factor 4; Transcription factor 4, isoform C; Transcription factor 4, isoform D; Transcription factor 4, isoform E; Transcription factor 4, isoform L; Transcription factor 4, isoform M; Transcription factor 4, isoform R;

抗原和靶标

免疫原:
Uniprot:

P15884(ITF2_HUMAN) >>Visit HPA database.

基因/基因ID:
TCF4(6925)
表达:
P15884 ITF2_HUMAN:

Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain.

描述:
LEF1 and TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3, and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers including colon cancer (4,5).
序列:
MHHQQRMAALGTDKELSDLLDFSAMFSPPVSSGKNGPTSLASGHFTGSNVEDRSSSGSWGNGGHPSPSRNYGDGTPYDHMTSRDLGSHDNLSPPFVNSRIQSKTERGSYSSYGRESNLQGCHQQSLLGGDMDMGNPGTLSPTKPGSQYYQYSSNNPRRRPLHSSAMEVQTKKVRKVPPGLPSSVYAPSASTADYNRDSPGYPSSKPATSTFPSSFFMQDGHHSSDPWSSSSGMNQPGYAGMLGNSSHIPQSSSYCSLHPHERLSYPSHSSADINSSLPPMSTFHRSGTNHYSTSSCTPPANGTDSIMANRGSGAAGSSQTGDALGKALASIYSPDHTNNSFSSNPSTPVGSPPSLSAGTAVWSRNGGQASSSPNYEGPLHSLQSRIEDRLERLDDAIHVLRNHAVGPSTAMPGGHGDMHGIIGPSHNGAMGGLGSGYGTGLLSANRHSLMVGTHREDGVALRGSHSLLPNQVPVPQLPVQSATSPDLNPPQDPYRGMPPGLQGQSVSSGSSEIKSDDEGDENLQDTKSSEDKKLDDDKKDIKSITSNNDDEDLTPEQKAEREKERRMANNARERLRVRDINEAFKELGRMVQLHLKSDKPQTKLLILHQAVAVILSLEQQVRERNLNPKAACLKRREEEKVSSEPPPLSLAGPHPGMGDASNHMGQM

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
60
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain.

亚基结构:

Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or heterooligomers with myogenin. Interacts with HIVEP2. Interacts with NEUROD2 (By similarity). Interacts with AGBL1. Interacts with BHLHA9.

蛋白家族:

the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

文献引用

1). A Single-Cell RNA Sequencing Guided Multienzymatic Hydrogel Design for Self-Regenerative Repair in Diabetic Mandibular Defects. Advanced materials (Deerfield Beach, Fla.), 2024 (PubMed: 39436107) [IF=27.4]
2). MicroRNA-32-5p promotes the proliferation and metastasis of gastric cancer cells. Scientific reports, 2025 (PubMed: 39833289) [IF=3.8]
3). miR-140-y targets TCF4 to regulate the Wnt signaling pathway and promote embryonic feather follicle development in Hungarian white goose. Poultry science, 2024 (PubMed: 38350393) [IF=3.8]

Application: WB    Species: goose    Sample: GEDFs

Figure 6. miR-140-y directly regulates target gene TCF4. (A) RNAhybrid analysis demonstrates the putative target site between miR-140-y and TCF4 mRNA 3′-UTR. (B) Binding site and mutation site of miR-140-y and TCF4. (C) Luciferase assay in GEDFs cells co-transfected with pMir-TCF4 -3′-UTR-WT and miR-140-y mimics and pMir-TCF4 3′-UTR MT and miR-140-y mimics. (D) Changes in the expression level of miR-140-y in GEDFs after transfection with miR-140-y mimics and inhibitors. (E) Overexpression of miR-140-y can reduce the mRNA expression level of TCF4 in GEDFs cells; knockdown of miR-140-y increases the mRNA expression level of TCF4 in GEDFs cells. (F) Western blot of TCF4 in GEDFs after transfection with miR-140-y mimics and inhibitors. (G) Overexpression of miR-140-y can reduce the protein expression level of TCF4 in GEDFs cells; knockdown of miR-140-y Low increases the protein expression level of TCF4 in GEDFs cells. The data were shown as mean ± SEM, n = 3, ** p < 0.01.
4). SLC25A18 has prognostic value in colorectal cancer and represses Warburg effect and cell proliferation via Wnt signaling. American Journal of Cancer Research, 2020 (PubMed: 32509397) [IF=3.6]

Application: WB    Species: human    Sample:

Figure 3.| SLC25A18 negatively regulates genes expression related to aerobics glycolysis, cell proliferation, andWnt/β-catenin cascade. (B) Gene set enrichment analysis implied a negative relationship between highly expressed SLC25A18 and the enrichment of CTNNB1 target genes and Wnt/β-catenin Signaling (HALLMARK_ WNT_BETA_CATENIN_SIGNALING, REACTOME_SIGNALING_BY_WNT) in patients with CRC from the TCGA database, (C)which was also verified by western blotting. All experiments were conducted in triplicates.
5). Fucoidan suppresses proliferation and epithelial-mesenchymal transition process via Wnt/β-catenin signalling in hemangioma. Experimental dermatology, 2024 (PubMed: 38514926) [IF=3.5]
6). Role of Wnt/β-catenin signaling pathway in the repair of intestinal mucosa associated with crypt stem cell in a rat model of abdominal compartment syndrome. International Journal of Clinical and Experimental Pathology, 2017 [IF=1.1]

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