NTF4 Antibody - #DF6226

(1)   (1)  
产品: NTF4 抗体
货号: DF6226
描述: Rabbit polyclonal antibody to NTF4
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Dog
分子量: 22kDa; 22kD(Calculated).
蛋白号: P34130
RRID: AB_2838192

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
NTF4 Antibody detects endogenous levels of total NTF4.
RRID:
AB_2838192
引用格式: Affinity Biosciences Cat# DF6226, RRID:AB_2838192.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

GLC10; GLC1O; Neurotrophic factor 4; Neurotrophic factor 5; Neurotrophin 4; Neurotrophin 4/5; Neurotrophin 5 (neurotrophin 4/5); Neurotrophin 5; Neurotrophin-4; Neurotrophin-5; Neutrophic factor 4; Neutrophic factor 5; NT 4; NT 4/5; NT 5; NT-4; NT-5; NT4; NT4/5; NT4P; NT5; NTF4; NTF4_HUMAN; NTF5;

抗原和靶标

免疫原:
Uniprot:

P34130(NTF4_HUMAN) >>Visit HPA database.

基因/基因ID:
NTF4(4909)
表达:
P34130 NTF4_HUMAN:

Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues.

描述:
NT-4 is a member of the structurally related neurotrophin family of proteins, which includes β-NGF, BDNF and NT-3 (1). NT-4 is expressed in a number of cell types and tissues, including neuronal cells, normal breast epithelial cells, melanocytes, activated T cells, and granulocytes (1-5). NT-4 is required for the development of peripheral sensory neurons (6,7). NT-4 may be important for the development of long term memory (8). Increased NT-4 expression in melanoma cells promotes cell proliferation and migration (5). NT-4 is secreted from cells as a precursor protein, which is proteolytically cleaved into the mature form (1). NT-4 signaling is mediated through two distinct receptors, the neurotrophin receptor p75NTR and the Trk tyrosine kinase receptor TrkB. While all neurotrophins bind to the p75NTR receptor, NT-4 preferentially binds to the TrkB receptor (1).
序列:
MLPLPSCSLPILLLFLLPSVPIESQPPPSTLPPFLAPEWDLLSPRVVLSRGAPAGPPLLFLLEAGAFRESAGAPANRSRRGVSETAPASRRGELAVCDAVSGWVTDRRTAVDLRGREVEVLGEVPAAGGSPLRQYFFETRCKADNAEEGGPGAGGGGCRGVDRRHWVSECKAKQSYVRALTADAQGRVGWRWIRIDTACVCTLLSRTGRA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P34130 作为底物

Site PTM Type Enzyme
S8 Phosphorylation
S83 Phosphorylation

研究背景

功能:

Target-derived survival factor for peripheral sensory sympathetic neurons.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues.

蛋白家族:

Belongs to the NGF-beta family.

研究领域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

文献引用

1). Electroacupuncture improves neuronal plasticity through the A2AR/cAMP/PKA signaling pathway in SNL rats. Neurochemistry International, 2021 (PubMed: 33577869) [IF=4.4]

Application: WB    Species: rat    Sample: spinal cord

Fig. 8. EA decreased the protein expression levels of NTF3, NTF4, Synaptophysin and Synapsin I in SNL rats, while SCH58261 reversed this effect. (A), (B), (C), (D), (E) Protein expression levels of NTF3, NTF4, Synaptophysin and Synapsin I in the spinal cord and quantification data of the expression of NTF3/GAPDH, NTF4/ GAPDH, Synaptophysin/GAPDH and Synapsin I/GAPDH in each group. n = 5. Columns represent the mean ± SD. $$p < 0.01 vs. the S group; #p < 0.05, ##p < 0.01 vs. the M group; &&p < 0.01 vs. the M + EA group.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.